AB256475

Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Glutathione Peroxidase 3/GPx-3 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Transfected cell lysate - Mouse, Mouse, Rat samples.

View Alternative Names

Glutathione peroxidase 3, GPx-3, GSHPx-3, Plasma glutathione peroxidase, GPx-P, GSHPx-P, Gpx3

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung (PMID : 22355371) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/100 (4.80 μg/ml) dilution followed by ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining on rat kidney (PMID : 21493731) is observed.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat lung (PMID : 22355371) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat kidney (PMID : 21493731, 27435875) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/100 (4.80 μg/ml) dilution followed by ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining on mouse kidney (PMID : 21493731) is observed.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID : 21493731, 27435875) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

Lab

Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

This data was developed using ab256470, the same antibody clone in a different buffer formulation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (<a href='/en-us/products/primary-antibodies/glutathione-peroxidase-3-gpx-3-antibody-epr22815-112-ab256470'>ab256470</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a full-length mouse Gpx3 expression vector containing a His tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a full-length mouse Gpx5 expression vector containing a His tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a full-length mouse Gpx6 expression vector containing a His tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,36 kDa

false

Exposure time: 10s

Unconjugated

Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112]
Carrier free

Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (Capture)
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112]
Biotin

Biotin Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22815-112

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-Fr, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab256475 is the carrier-free version of ab256470.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione Peroxidase 3 also known as GPx-3 or 3GPx is an antioxidant enzyme important for protecting cells from oxidative damage by reducing hydrogen peroxide and organic hydroperoxides. It is a member of the glutathione peroxidase family and has a molecular mass of approximately 23 kDa. GPx-3 is expressed mainly in the kidney and subsequently released into the bloodstream playing an important role in its extracellular antioxidant defense mechanism.

Biological function summary

GPx-3 helps maintain cellular redox balance and is involved in detoxifying harmful oxidative agents. It does not form part of any larger complex but works independently to reduce peroxides with the help of glutathione an important reducing agent. GPx-3 has significant activities in plasma and extracellular fluids indicating its role in protecting the body from oxidative stress away from cellular environments.

Pathways

GPx-3 functions in the glutathione metabolic pathway and the defense system against oxidative stress. It works together with proteins such as glutathione reductase to recycle oxidized glutathione back to its reduced form allowing continuous protection against reactive oxygen species. This pathway is essential for maintaining balance between oxidative stress and antioxidant defense in the organism.

GPx-3 has been linked to cardiovascular diseases and certain types of cancer. Low levels of GPx-3 in plasma have been associated with increased risk of thrombosis as antioxidant defenses are compromised. In cancer studies altered GPx-3 expression is often observed thought to affect the progression of disease by changing the cellular redox environment. Other proteins that interact with GPx-3 during disease conditions include thioredoxin and catalase both influencing and being regulated by redox states in diseased tissues.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

See full target information Gpx3

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (AB256475)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise