Rat Recombinant Monoclonal Glutathione Peroxidase 4 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 8 publications.
Images
![Western blot - Anti-Glutathione Peroxidase 4 antibody [1B4] (AB252833), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-4-antibody-1b4-ab252833--western-blot-img30575.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glutathione Peroxidase 4 antibody [1B4] (AB252833)")
Publications
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- OncoTargets and therapy 16:867-8832023B7 Induces Apoptosis in Colorectal Cancer Cells by Regulating the Expression of Caspase-3 and Inhibits Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyi Zhang,Fengxi Li,Rong Li,Nan Zhao,Dianfeng Liu,Yuelin Xu,Lei Wang,Dongxu Wang,Ruihong ZhaoPubMed 37915320
- Methods in molecular biology (Clifton, N.J.) 2712:135-1482023Analysis of Protein Degradation in Ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuojun Zhang,Lili JiangPubMed 37578702
- International journal of biological sciences 19:2531-25502023Anomanolide C suppresses tumor progression and metastasis by ubiquitinating GPX4-driven autophagy-dependent ferroptosis in triple negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Mei Chen,Wei Xu,Yang Liu,Jia-Hui Zhang,Yuan-Yuan Yang,Zhi-Wen Wang,De-Juan Sun,Hua Li,Bo Liu,Li-Xia ChenPubMed 37215985
- Annals of translational medicine 10:13902023Ferroptosis-related genes with post-transcriptional regulation mechanisms in hepatocellular carcinoma determined by bioinformatics and experimental validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenfei Zhu,Cheng Gao,Qiuqi Feng,Haitao Guan,Jianjun Wu,Hrishikesh Samant,Fan Yang,Xuehao WangPubMed 36660631
- Oxidative medicine and cellular longevity 2022:52369082022Attenuation of the Severity of Acute Respiratory Distress Syndrome by Pomiferin through Blocking Inflammation and Oxidative Stress in an AKT/Foxo1 Pathway-Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Tang,Zetian Yang,Hui Feng,Xuefeng Zhou,Ming MaoPubMed 36471865
- Cell death discovery 8:862022NVP-AUY922 alleviates radiation-induced lung injury via inhibition of autophagy-dependent ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Li,Dongming Wu,Shihua Deng,Jin Li,Feng Zhang,Ye Zou,Ting Zhang,Ying XuPubMed 35220409
- Oncology letters 23:672022MicroRNA-15a promotes prostate cancer cell ferroptosis by inhibiting GPX4 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPo Xu,Ying Wang,Zhe Deng,Zhibo Tan,Xiaojuan PeiPubMed 35069876
- Cell metabolism 8:182-32008Membrane redox state and apoptosis: death by peroxide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph LoscalzoPubMed 18762018
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.976 µg/mL | Notes - |
Species Rat | Dilution info 0.976 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (PubMed:29290465). Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide (By similarity). Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation (PubMed:12566075). Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species (PubMed:12566075, PubMed:24439385, PubMed:25402683, PubMed:25922076, PubMed:29290465). The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis (PubMed:29290465). The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures (PubMed:29290465). May be required to protect cells from the toxicity of ingested lipid hydroperoxides (PubMed:12566075). Required for normal sperm development and male fertility (PubMed:19783653, PubMed:25922076). Essential for maturation and survival of photoreceptor cells (PubMed:22207760). Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion (PubMed:25824823). Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism (By similarity). Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway (By similarity). Can also reduce small soluble hydroperoxides such as H2O2 and tert-butyl hydroperoxide (PubMed:12566075). Isoform Cytoplasmic. Specifically able to suppress the production of leukotriene and prostaglandin in response to several stimuli by reducing fatty acid hydroperoxide. Isoform Mitochondrial. Specifically required to prevent mitochondrial cell death by mediating reduction of cardiolipin hydroperoxide (By similarity). Also required for normal sperm development and male fertility (PubMed:19417079). Isoform Nuclear. Required for male fertility by stabilizing the condensed chromatin in sperm nuclei (PubMed:12566075).
Alternative names
Phospholipid hydroperoxide glutathione peroxidase GPX4, PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4, Gpx4
Recommended products
Rat Recombinant Monoclonal Glutathione Peroxidase 4 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 8 publications.
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 1B4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutathione Peroxidase 4 (GPX4) also known as PHGPx acts as an important enzyme in protecting cells from oxidative damage by reducing lipid hydroperoxides to their alcohols and free hydrogen peroxide to water. It has a mass of approximately 20 kDa. GPX4 is widely expressed in various tissues including the brain testis kidney and liver underlining its significance in different physiological processes. GPX4 is essential in cellular anti-oxidant defense making it a potential marker in studies using GPX4 western blot techniques.
- Biological function summary
GPX4 plays an important role in protecting cellular membranes from lipid peroxidation by acting alone which differentiates it from other glutathione peroxidases that work in a complex. It is necessary for the production of glutathione products and helps maintain redox homeostasis by converting glutathione peroxides. This action prevents cell death pathways linked to oxidative stress and ensures cellular integrity particularly in sensitive tissues like the brain and reproductive organs.
- Pathways
GPX4 integrates into important cellular antioxidant pathways. Specifically it functions within the glutathione metabolic pathway collaborating with related proteins like glutathione synthetase and glutathione reductase. GPX4 is also involved in ferroptosis regulation a cell death pathway critically linked to glutathione levels. It helps balance redox-sensitive signaling and metabolic activities demonstrating synergy with other antioxidant enzymes in the cellular response to oxidative stress.
- Associated diseases and disorders
Research connects GPX4 deficiency to neurological disorders and cancer. Its important antioxidative activity makes it significant in preventing neurodegenerative diseases such as Alzheimer's where it interacts with proteins like PINK1. GPX4 also shows relevance in cancer as its altered expression may increase vulnerability to cell death suggesting its potential role in chemotherapy resistance and therapy development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Glutathione Peroxidase 4 antibody [1B4] (ab252833), expandable thumbnail](https://content.abcam.com/products/images/glutathione-peroxidase-4-antibody-1b4-ab252833--western-blot-img30575.jpg "Western blot - Anti-Glutathione Peroxidase 4 antibody [1B4] (ab252833)")
Western blot - Anti-Glutathione Peroxidase 4 antibody [1B4] (ab252833)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Glutathione Peroxidase 4 antibody [1B4] (ab252833) at 0.976 µg/mL
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: Rat testis tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 22 kDa
Observed band size: 17 kDa
Exposure time: 1s
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