AB137513

Anti-Glutathione Reductase antibody

Name: Anti-Glutathione Reductase antibody (ab137513) | Abcam
Brand: Abcam Limited
SKU: AB137513
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal Glutathione Reductase antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GSR aa 150-450.

View Alternative Names

GLUR, GRD1, GSR, GR, GRase

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Reductase antibody (AB137513)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Reductase antibody (AB137513)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for Glutathione reductase protein at cytoplasm and nucleus with ab137513 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Reductase antibody (AB137513)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for Glutathione reductase protein at cytoplasm and nucleus with ab137513 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Glutathione Reductase antibody (AB137513)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Glutathione Reductase antibody (ab137513) at 1/1000 dilution

All lanes:

HCT116 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 55 kDa

false

Supplier Data

Western blot - Anti-Glutathione Reductase antibody (AB137513)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Glutathione Reductase antibody (ab137513) at 1/1000 dilution

All lanes:

Mouse eye tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 55 kDa

false

Supplier Data

Western blot - Anti-Glutathione Reductase antibody (AB137513)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Glutathione Reductase antibody (ab137513) at 1/1000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GSR aa 150-450. The exact immunogen used to generate this antibody is proprietary information.

P00390

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione reductase also known as GSR is an enzyme that plays a mechanical role in maintaining the redox balance within cells. This enzyme reduces glutathione disulfide (GSSG) to the sulfhydryl form GSH which is essential for detoxifying reactive oxygen species. Glutathione reductase has a molecular weight of approximately 55 kDa. It is widely expressed in various tissues including the liver where it participates in detoxification processes. The enzyme operates in the cytosol and mitochondria reflecting its necessity across different cellular compartments.

Biological function summary

The enzyme sustains a supply of reduced glutathione which is important for antioxidant defense and cellular protection against oxidative stress. As a critical player in the glutathione system it is not part of a larger complex but interacts significantly within the cellular antioxidant framework. It supports important functions like protein folding and DNA synthesis by keeping the cellular redox environment stable. The enzyme’s activity ensures that glutathione an important antioxidant with a molecular weight of 307.32 Da remains in its active form to fulfill its protective roles.

Pathways

The enzyme is deeply integrated into the pentose phosphate pathway which generates NADPH necessary for the reduction of glutathione disulfide. It also connects to the glutathione metabolism pathway where it maintains homeostasis through GSH regeneration. Glutathione reductase intersects with other proteins like glutathione peroxidase (GPx) which uses GSH to reduce hydrogen peroxide displaying a coordinated effort in mitigating oxidative damage.

Deficiency or dysfunction of this enzyme links to conditions such as hemolytic anemia where reduced enzyme activity leads to higher susceptibility to oxidative damage in red blood cells. Additionally its dysfunction associates with neurodegenerative disorders where oxidative stress plays a critical role. The enzyme's function relates to proteins like superoxide dismutase (SOD) in disease pathways where both contribute to managing oxidative stress. Understanding alterations in glutathione reductase activity may offer insights into therapeutic strategies for these oxidative stress-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Constitutes the major mechanism to maintain a high GSH : GSSG ratio in the cytosol.

See full target information GSR

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 18:1542938 PubMed39958993

2025

Impaired peroxisomal beta-oxidation in microglia triggers oxidative stress and impacts neurons and oligodendrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Tawbeh,Catherine Gondcaille,Fatima-Ezzahra Saih,Quentin Raas,Damien Loichot,Yannick Hamon,Céline Keime,Alexandre Benani,Francesca Di Cara,Mustapha Cherkaoui-Malki,Pierre Andreoletti,Stéphane Savary

Redox biology 75:103276 PubMed39053265

2024

Metastatic breast cancer cells are metabolically reprogrammed to maintain redox homeostasis during metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Biondini,Camille Lehuédé,Sébastien Tabariès,Matthew G Annis,Alain Pacis,Eric H Ma,Christine Tam,Brian E Hsu,Yannick Audet-Delage,Afnan Abu-Thuraia,Charlotte Girondel,Valerie Sabourin,Stephanie P Totten,Mariana de Sá Tavares Russo,Gaëlle Bridon,Daina Avizonis,Marie-Christine Guiot,Julie St-Pierre,Josie Ursini-Siegel,Russell Jones,Peter M Siegel

Translational stroke research 12:1045-1054 PubMed33479917

2021

Endoplasmic Reticulum Interaction Supports Energy Production and Redox Homeostasis in Mitochondria Released from Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hyun Park,Eng H Lo,Kazuhide Hayakawa

Stroke 51:3142-3146 PubMed32819193

2020

Placental Mitochondria Therapy for Cerebral Ischemia-Reperfusion Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiko Nakamura,Eng H Lo,Kazuhide Hayakawa

The Journal of physiology 597:869-887 PubMed30556208

2018

Induced in vivo knockdown of the Brca1 gene in skeletal muscle results in skeletal muscle weakness.

Applications

Unspecified application

Species

Unspecified reactive species

Michael D Tarpey,Ana P Valencia,Kathryn C Jackson,Adam J Amorese,Nicholas P Balestrieri,Randall H Renegar,Stephen J P Pratt,Terence E Ryan,Joseph M McClung,Richard M Lovering,Espen E Spangenburg

Molecular medicine reports 12:5262-6 PubMed26133229

2015

Involvement of Kruppel-like factor 9 in bleomycin-induced pulmonary toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Gu,Yan-Bing Wu,Li-Hui Wang,Jia-Ning Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glutathione Reductase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Reductase antibody (AB137513)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Reductase antibody (AB137513)

Western blot - Anti-Glutathione Reductase antibody (AB137513)

Western blot - Anti-Glutathione Reductase antibody (AB137513)

Western blot - Anti-Glutathione Reductase antibody (AB137513)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise