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AB128933

Anti-Glutathione Reductase antibody [EPR7237]

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(6 Publications)

Rabbit Recombinant Monoclonal Glutathione Reductase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

GLUR, GRD1, GSR, GR, GRase

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)
  • ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

ab128933, at a 1/100 dilution, staining Human Glutathione Reductase in HeLa cells, by Immunofluorescence.

Flow Cytometry (Intracellular) - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Glutathione Reductase with purified ab128933 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)
  • WB

Unknown

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (ab128933) at 1/10000 dilution

Lane 1:

Caco-2 cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 56 kDa

Observed band size: 50 kDa

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Western blot - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)
  • WB

Lab

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Glutathione Reductase knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab128933 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab128933 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used, along with additional cross-reactive bands. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE. ab128933 and ab8245 (loading control to GAPDH) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7237

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione reductase also known as GSR is an enzyme that plays a mechanical role in maintaining the redox balance within cells. This enzyme reduces glutathione disulfide (GSSG) to the sulfhydryl form GSH which is essential for detoxifying reactive oxygen species. Glutathione reductase has a molecular weight of approximately 55 kDa. It is widely expressed in various tissues including the liver where it participates in detoxification processes. The enzyme operates in the cytosol and mitochondria reflecting its necessity across different cellular compartments.
Biological function summary

The enzyme sustains a supply of reduced glutathione which is important for antioxidant defense and cellular protection against oxidative stress. As a critical player in the glutathione system it is not part of a larger complex but interacts significantly within the cellular antioxidant framework. It supports important functions like protein folding and DNA synthesis by keeping the cellular redox environment stable. The enzyme’s activity ensures that glutathione an important antioxidant with a molecular weight of 307.32 Da remains in its active form to fulfill its protective roles.

Pathways

The enzyme is deeply integrated into the pentose phosphate pathway which generates NADPH necessary for the reduction of glutathione disulfide. It also connects to the glutathione metabolism pathway where it maintains homeostasis through GSH regeneration. Glutathione reductase intersects with other proteins like glutathione peroxidase (GPx) which uses GSH to reduce hydrogen peroxide displaying a coordinated effort in mitigating oxidative damage.

Deficiency or dysfunction of this enzyme links to conditions such as hemolytic anemia where reduced enzyme activity leads to higher susceptibility to oxidative damage in red blood cells. Additionally its dysfunction associates with neurodegenerative disorders where oxidative stress plays a critical role. The enzyme's function relates to proteins like superoxide dismutase (SOD) in disease pathways where both contribute to managing oxidative stress. Understanding alterations in glutathione reductase activity may offer insights into therapeutic strategies for these oxidative stress-related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Maintains high levels of reduced glutathione in the cytosol.
See full target information GSR
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in reproductive health 6:1479568 PubMed39726694

2024

Mitochondrial dysfunction signatures in idiopathic primary male infertility: a validated proteomics-based diagnostic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Raneen Sawaid Kaiyal,Sromona D Mukherjee,Manesh Kumar Panner Selvam,Aaron W Miller,Sarah C Vij,Scott D Lundy

Cell reports. Medicine 4:101224 PubMed37797616

2023

Glutathione dynamics is a potential predictive and therapeutic trait for neoadjuvant chemotherapy response in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

YongHwan Kim,Hyein Ju,Seung-Yeon Yoo,Jinahn Jeong,Jinbeom Heo,Seungun Lee,Ja-Min Park,Sun Young Yoon,Se Un Jeong,Jinyoung Lee,HongDuck Yun,Chae-Min Ryu,Jinah Lee,Yun Ji Nam,Hyungu Kwon,Jaekyoung Son,Gowun Jeong,Ji-Hye Oh,Chang Ohk Sung,Eui Man Jeong,Jaehoon An,Sungho Won,Bumsik Hong,Jae Lyun Lee,Yong Mee Cho,Dong-Myung Shin

Cancers 14: PubMed35626147

2022

Mutational Activation of the NRF2 Pathway Upregulates Kynureninase Resulting in Tumor Immunosuppression and Poor Outcome in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes F Fahrmann,Ichidai Tanaka,Ehsan Irajizad,Xiangying Mao,Jennifer B Dennison,Eunice Murage,Julian Casabar,Jeffrey Mayo,Qian Peng,Muge Celiktas,Jody V Vykoukal,Soyoung Park,Ayumu Taguchi,Oliver Delgado,Satyendra C Tripathi,Hiroyuki Katayama,Luisa Maren Solis Soto,Jaime Rodriguez-Canales,Carmen Behrens,Ignacio Wistuba,Samir Hanash,Edwin J Ostrin

Science advances 6:eaba1334 PubMed32490200

2020

Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jisun Lim,Jinbeom Heo,Hyein Ju,Ji-Woong Shin,YongHwan Kim,Seungun Lee,Hwan Yeul Yu,Chae-Min Ryu,HongDuck Yun,Sujin Song,Ki-Sung Hong,Hyung-Min Chung,Hwa-Ryeon Kim,Jae-Seok Roe,Kihang Choi,In-Gyu Kim,Eui Man Jeong,Dong-Myung Shin

The Journal of biological chemistry 291:11185-97 PubMed27048652

2016

Compartment-specific Control of Reactive Oxygen Species Scavenging by Antioxidant Pathway Enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Swati Dey,Agnieszka Sidor,Brian O'Rourke

Oncogene 35:2235-46 PubMed26234675

2015

JAK2V617F mediates resistance to DNA damage-induced apoptosis by modulating FOXO3A localization and Bcl-xL deamidation.

Applications

Unspecified application

Species

Unspecified reactive species

J S Ahn,J Li,E Chen,D G Kent,H J Park,A R Green
View all publications
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Product promise

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