AB124995

Anti-Glutathione Reductase antibody [EPR7238]

Name: Anti-Glutathione Reductase antibody [EPR7238] (ab124995) | Abcam
Brand: Abcam Limited
SKU: AB124995
Price: 370 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(14 Publications)

Rabbit Recombinant Monoclonal Glutathione Reductase antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 14 publications.

View Alternative Names

GLUR, GRD1, GSR, GR, GRase

4 Images

Lab

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (AB124995)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Glutathione Reductase knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab124995 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab124995 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE. ab124995 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)

Predicted band size: 56 kDa

false

Unknown

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (AB124995)

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 56 kDa

false

Lab

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (AB124995)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution

Lane 1:

mouse brain at 20 µg

Lane 2:

rat brain at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa,141 kDa,15 kDa,162 kDa,172 kDa,18 kDa,21 kDa,22 kDa,25 kDa,27 kDa,29 kDa,32 kDa,34 kDa,35 kDa,351 kDa,39 kDa,45 kDa,46 kDa,53 kDa,55 kDa,56 kDa,59 kDa,60 kDa,63 kDa,66 kDa,83 kDa,84 kDa,98 kDa

Observed band size: 50 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (AB124995)

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate

Lane 2:

Mouse heart tissue lysate

Lane 3:

Mouse kidney tissue lysate

Lane 4:

Mouse spleen tissue lysate

Predicted band size: 56 kDa

Observed band size: 50 kDa

false

Exposure time: 3min

Carrier free

Anti-Glutathione Reductase antibody [EPR7238] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7238

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione reductase also known as GSR is an enzyme that plays a mechanical role in maintaining the redox balance within cells. This enzyme reduces glutathione disulfide (GSSG) to the sulfhydryl form GSH which is essential for detoxifying reactive oxygen species. Glutathione reductase has a molecular weight of approximately 55 kDa. It is widely expressed in various tissues including the liver where it participates in detoxification processes. The enzyme operates in the cytosol and mitochondria reflecting its necessity across different cellular compartments.

Biological function summary

The enzyme sustains a supply of reduced glutathione which is important for antioxidant defense and cellular protection against oxidative stress. As a critical player in the glutathione system it is not part of a larger complex but interacts significantly within the cellular antioxidant framework. It supports important functions like protein folding and DNA synthesis by keeping the cellular redox environment stable. The enzyme’s activity ensures that glutathione an important antioxidant with a molecular weight of 307.32 Da remains in its active form to fulfill its protective roles.

Pathways

The enzyme is deeply integrated into the pentose phosphate pathway which generates NADPH necessary for the reduction of glutathione disulfide. It also connects to the glutathione metabolism pathway where it maintains homeostasis through GSH regeneration. Glutathione reductase intersects with other proteins like glutathione peroxidase (GPx) which uses GSH to reduce hydrogen peroxide displaying a coordinated effort in mitigating oxidative damage.

Deficiency or dysfunction of this enzyme links to conditions such as hemolytic anemia where reduced enzyme activity leads to higher susceptibility to oxidative damage in red blood cells. Additionally its dysfunction associates with neurodegenerative disorders where oxidative stress plays a critical role. The enzyme's function relates to proteins like superoxide dismutase (SOD) in disease pathways where both contribute to managing oxidative stress. Understanding alterations in glutathione reductase activity may offer insights into therapeutic strategies for these oxidative stress-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Constitutes the major mechanism to maintain a high GSH : GSSG ratio in the cytosol.

See full target information GSR

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:28829 PubMed40770005

2025

Explore the role of CBS in stomach adenocarcinoma based on the sulfur-containing amino acid metabolism network.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhang,Yingchao Liu,Yajuan Lu,Aoli Deng,Jinghao Cao,Hangqi Huang,Cuiyun Wu,Jing Du,Yanchun Li,Jun Xia

eLife 14: PubMed40464376

2025

Targeting SLC7A11-mediated cysteine metabolism for the treatment of trastuzumab-resistant HER2-positive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yijia Hua,Ningjun Duan,Chunxiao Sun,Fan Yang,Min Tian,Yanting Sun,Shuhan Zhao,Jue Gong,Qian Liu,Xiang Huang,Yan Liang,Ziyi Fu,Wei Li,Yongmei Yin

Veterinary research 56:1 PubMed39748435

2025

Glutathione reductase modulates endogenous oxidative stress and affects growth and virulence in Avibacterium paragallinarum.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhi,Chen Mei,Zhenyi Liu,Ying Liu,Hongjun Wang

The Kaohsiung journal of medical sciences 40:1057-1067 PubMed39548856

2024

Nuciferine inhibits osteoclast formation through suppressing glycolysis metabolic programming and ROS production.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Hui Guo,Guan-Qi Zhen,Feng Wu,Yun-Peng Lv,Jing-Long Yan,Jia-Ning Zu,Cheng-Chao Song

Redox biology 69:103007 PubMed38150993

2023

Inhibiting the compensatory elevation of xCT collaborates with disulfiram/copper-induced GSH consumption for cascade ferroptosis and cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhang,Chaoting Zhou,Xueying Ren,Qiangan Jing,Yan Gao,Chen Yang,Yuhuan Shen,Yi Zhou,Wanye Hu,Feifan Jin,Haifeng Xu,Lingyan Yu,Yingchao Liu,Xiangmin Tong,Yanchun Li,Ying Wang,Jing Du

Journal of neurotrauma 39:1075-1089 PubMed35373589

2022

Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew N Stewart,Ethan P Glaser,Caitlin A Mott,William M Bailey,Patrick G Sullivan,Samir P Patel,John C Gensel

Bioengineered 13:13089-13107 PubMed35615982

2022

CDC25C is a prognostic biomarker and correlated with mitochondrial homeostasis in pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoting Zhou,Luyang Wang,Wanye Hu,Lusheng Tang,Ping Zhang,Yan Gao,Jing Du,Yanchun Li,Ying Wang

Antioxidants (Basel, Switzerland) 10: PubMed34679737

2021

Exploring the Leukemogenic Potential of GATA-1, the Shorter Isoform of GATA-1: Novel Insights into Mechanisms Hampering Respiratory Chain Complex II Activity and Limiting Oxidative Phosphorylation Efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Trombetti,Raffaele Sessa,Rosa Catapano,Laura Rinaldi,Alessandra Lo Bianco,Antonio Feliciello,Paola Izzo,Michela Grosso

Frontiers in pharmacology 12:734774 PubMed34566656

2021

The Novel Antioxidant Compound JSH-23 Prevents Osteolysis by Scavenging ROS During Both Osteoclastogenesis and Osteoblastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Liangwei Mei,Yi Zheng,Teng Ma,Bing Xia,Xue Gao,Yiming Hao,Zhuojing Luo,Jinghui Huang

Folia neuropathologica 58:253-264 PubMed33099295

2020

Inhibition of MAPT enhances the effect of bexarotene and attenuates the damage after traumatic brain injury using in vivo and in vitro experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Haihai Dong,Haitao Wang,Liang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glutathione Reductase antibody [EPR7238]

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (AB124995)

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (AB124995)

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (AB124995)

Western blot - Anti-Glutathione Reductase antibody [EPR7238] (AB124995)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise