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AB53942

Anti-Glutathione S Transferase mu antibody

4

(2 Reviews)

|

(12 Publications)

Goat Polyclonal GSTM1 antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Hamster, Human, Rabbit samples. Cited in 12 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Gstm1.

View Alternative Names

GST1, GSTM1, Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4

1 Images
Western blot - Anti-Glutathione S Transferase mu antibody (AB53942)
  • WB

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Western blot - Anti-Glutathione S Transferase mu antibody (AB53942)

Lane 1. Molecular weight marker
Lane 2. GST μ standard

A 1 : 2000 dilution is recommended for ECL method with overnight incubation at room temperature.

All lanes:

Western blot - Anti-Glutathione S Transferase mu antibody (ab53942)

Predicted band size: 26 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rabbit, Rat, Mouse, Hamster, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Rat Gstm1.

P04905

Specificity

ab53942 is specific for Glutathione S Transferase (GST) mu. It reacts with subtype 3-3 (M1) and subtype 4-4 (M2).

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Reactivity data

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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
IgG fraction was prepared from the antisera using the caprylic acid precipitation method.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.7% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione S-transferase mu 1 also known as GSTM1 is an important enzyme involved in detoxification processes. This enzyme belongs to the glutathione S-transferases family which plays an important role in catalyzing the conjugation of glutathione to various electrophilic compounds. GSTM1 has a molecular mass of approximately 26 kDa and is primarily expressed in the liver but it is also present in other tissues such as the kidney and erythrocytes. GSTM1's active site involves a cysteine residue that facilitates the transfer of glutathione to potential harmful substances aiding in their subsequent excretion from the body.
Biological function summary

The enzyme contributes to maintaining cellular homeostasis by neutralizing toxins and protecting against oxidative stress. GSTM1 is part of a larger family of enzymes each with specific substrate affinities but all contributing to the detoxification of xenobiotics and carcinogens. This process is critically important to protect cells from environmental and oxidative damage. The enzyme highly expresses in liver tissue highlights its role in processing and eliminating harmful substances from the body.

Pathways

GSTM1 plays a significant role in the xenobiotic metabolism pathway where it participates in the detoxification and clearance of various xenobiotic substances. Another important pathway involving GSTM1 is the glutathione metabolism pathway. Within these pathways GSTM1 interacts with other glutathione S-transferases and proteins such as cytochrome P450s which also contribute to the metabolism of endogenous and exogenous compounds. The interaction with these proteins highlights its participation in a coordinated defense mechanism against chemical insults.

An absence or deficiency of GSTM1 is associated with an increased risk of conditions such as asthma and certain cancers particularly bladder cancer. The lack of GSTM1's detoxifying activity may increase susceptibility to environmental toxins and carcinogens. This enzyme together with other glutathione S-transferases plays a role in mitigating oxidative stress related to these conditions. Through its interactions with cytochrome P450 enzymes GSTM1 influences metabolic activation and deactivation of various potential carcinogens highlighting its relevance in disease prevention strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276).
See full target information GSTM1
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Experimental and molecular pathology 141:104955 PubMed39938190

2025

Hyperinsulinemic male LEW.1WR1 rats show early signs of impaired liver metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Madushika Wimalarathne,Bailey L Bowser,Albert B Arul,Quiana C Wilkerson-Vidal,Moses A David,Emily C Hunt,Helen Gibson,Renã A S Robinson,Sharifa T Love-Rutledge

PloS one 17:e0276879 PubMed36378690

2022

The autophagic protein p62 is a target of reactive aldehydes in human and murine cholestatic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Colin T Shearn,Aimee L Anderson,Michael W Devereux,David J Orlicky,Cole Michel,Dennis R Petersen,Colin G Miller,Sanjiv Harpavat,Edward E Schmidt,Ronald J Sokol

Journal of clinical laboratory analysis 36:e24304 PubMed35312115

2022

Hsa_circ_0007142 contributes to cisplatin resistance in esophageal squamous cell carcinoma via miR-494-3p/LASP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Na Chang,Ning Ge,Yufei Zhao,Liu Yang,Wei Qin,Yayun Cui

Science signaling 12: PubMed30783009

2019

Glutathione -transferases promote proinflammatory astrocyte-microglia communication during brain inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Ichi Kano,Eric Y Choi,Eisuke Dohi,Swati Agarwal,Daniel J Chang,Ashley M Wilson,Brian D Lo,Indigo V L Rose,Santiago Gonzalez,Takashi Imai,Akira Sawa

Archives of medical science : AMS 14:1112-1118 PubMed30154895

2018

Plumbagin protects against hydrogen peroxide-induced neurotoxicity by modulating NF-κB and Nrf-2.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Kuan-Hong,Li Bai-Zhou

Xenobiotica; the fate of foreign compounds in biological systems 48:867-881 PubMed28882082

2017

Changes in hepatic phase I and phase II biotransformation enzyme expression and glutathione levels following atrazine exposure in female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Arthur D Zimmerman,Charles B Breckenridge,Kun D Yi,Pragati Sawhney Coder,Desiree Wanders,Robert L Judd,Chad D Foradori

Kidney international 88:1261-1273 PubMed26422507

2015

Integrated transcriptomic and proteomic analyses uncover regulatory roles of Nrf2 in the kidney.

Applications

WB

Species

Mouse

Luke M Shelton,Adam Lister,Joanne Walsh,Rosalind E Jenkins,Michael H L Wong,Cliff Rowe,Emanuele Ricci,Lorenzo Ressel,Yongxiang Fang,Philippe Demougin,Vanja Vukojevic,Paul M O'Neill,Christopher E Goldring,Neil R Kitteringham,B Kevin Park,Alex Odermatt,Ian M Copple

Molecular neurobiology 53:4939-60 PubMed26374549

2015

Astrocytes Surviving Severe Stress Can Still Protect Neighboring Neurons from Proteotoxic Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda M Gleixner,Jessica M Posimo,Deepti B Pant,Matthew P Henderson,Rehana K Leak

PloS one 8:e62497 PubMed23741294

2013

Glutathione S-transferases interact with AMP-activated protein kinase: evidence for S-glutathionylation and activation in vitro.

Applications

IP

Species

Rat

Anna Klaus,Sarah Zorman,Alexandre Berthier,Cécile Polge,Sacnicte Ramirez,Sylvie Michelland,Michel Sève,Didier Vertommen,Mark Rider,Nicolas Lentze,Daniel Auerbach,Uwe Schlattner

Journal of molecular recognition : JMR 26:32-7 PubMed23280615

2013

Glutathione S-transferase π complexes with and stimulates Na⁺,K⁺-ATPase.

Applications

Unspecified application

Species

Unspecified reactive species

Hideo Ochiai,Hiroshi Eguchi,Shunsuke Noguchi,Yutaro Hayashi,Hideaki Nishino,Masaru Kawamura,Chau H Wu
View all publications
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