AB88604

Anti-glutathione S transferase Omega 1/p28 antibody

Name: Anti-glutathione S transferase Omega 1/p28 antibody (ab88604) | Abcam
Brand: Abcam Limited
SKU: AB88604
Price: 545 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Mouse Polyclonal glutathione S transferase Omega 1/p28 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GSTO1.

View Alternative Names

GSTTLP28, GSTO1, Glutathione S-transferase omega-1, GSTO-1, Glutathione S-transferase omega 1-1, Glutathione-dependent dehydroascorbate reductase, Monomethylarsonic acid reductase, S-(Phenacyl)glutathione reductase, GSTO 1-1, MMA(V) reductase, SPG-R

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

ab88604 at 10μg/ml staining glutathione S transferase Omega 1/p28 in HeLa cells by immunofluorescence.

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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (ab88604) at 1 µg/mL

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Jurkat cell lysate at 50 µg

Predicted band size: 28 kDa

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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

All lanes:

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (ab88604) at 1 µg/mL

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HeLa cell lysate at 50 µg

Predicted band size: 28 kDa

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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (ab88604) at 1 µg/mL

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NIH 3T3 cell lysate at 50 µg

Predicted band size: 28 kDa

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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (ab88604) at 1 µg/mL

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PC-12 cell lysate at 50 µg

Predicted band size: 28 kDa

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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

All lanes:

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (ab88604) at 1 µg/mL

Lane 1:

Glutathione S transferase Omega 1/p28 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Predicted band size: 28 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GSTO1.

P78417

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione S-transferase Omega 1 also known as GSTO1 or p28 is an enzyme that plays an important role in detoxification processes. It belongs to the omega class of glutathione S-transferases and has a molecular mass of approximately 28 kDa. This enzyme is expressed in various human tissues including the liver kidney and brain where it performs important metabolic functions. GSTO1 is not only involved in catalyzing the reduction of hydroxyecdysone metabolites such as ecdysterone (20-hydroxyecdysone) but also participates in modulating cellular responses to oxidative stress.

Biological function summary

GSTO1 is part of a complex network of proteins involved in protecting cells from oxidative damage. This enzyme takes part in antioxidant defense by catalyzing the conjugation of glutathione with harmful compounds facilitating their elimination. As a part of detoxification processes GSTO1 assists in maintaining cellular redox balance. Such activities are essential for several physiological functions including the metabolism of steroid compounds which can relate to ecdysteroid steroid activity. Its activity influences not only metabolic stability but also cell survival and integrity.

Pathways

GSTO1 functions in significant signaling pathways particularly in glutathione metabolism and stress response pathways. It interacts with proteins such as glutaredoxins and thioredoxins playing a role in regulating redox-sensitive signaling. Through its involvement in glutathione pathways GSTO1 helps mitigate damage from reactive oxygen species (ROS) and supports cellular repair mechanisms. Its actions link it with the broader antioxidant networks that maintain cellular homeostasis and modulate stress-induced pathways.

GSTO1 has connections to neurological and inflammatory conditions. For instance alterations in GSTO1 expression have been associated with Alzheimer's disease where oxidative stress plays a pivotal role. Similarly it seems implicated in inflammatory disorders due to its capacity to modulate inflammatory responses. Dysfunctional GSTO1 activity can result in an imbalance in oxidative stress pathways linking it with disease-related proteins like amyloid beta in neurodegenerative diseases and inflammatory cytokines in immune-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Also has glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.

See full target information GSTO1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 10:314 PubMed30873169

2019

T Cell Receptor (TCR)-Induced PLC-γ1 Sumoylation via PIASxβ and PIAS3 SUMO E3 Ligases Regulates the Microcluster Assembly and Physiological Function of PLC-γ1.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Long Wang,Jia-Qi Liang,Bei-Ni Gong,Ji-Ji Xie,Yu-Ting Yi,Xin Lan,Yingqiu Li

International journal of dermatology 56:225-231 PubMed27613512

2016

An investigation of cytochrome p450 (CYP) and glutathione S-transferase (GST) isoenzyme protein expression and related interactions with phototherapy in patients with psoriasis vulgaris.

Applications

Unspecified application

Species

Unspecified reactive species

Ayse S Karadag,Tuğba K Uzunçakmak,Seyma Ozkanli,Serpil Oguztuzun,Busra Moran,Ozge Akbulak,Emin Ozlu,Itir E Zemheri,Serap G Bilgili,Necmettin Akdeniz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-glutathione S transferase Omega 1/p28 antibody

Immunocytochemistry/ Immunofluorescence - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody (AB88604)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise