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Rabbit Recombinant Monoclonal glutathione S transferase Omega 1/p28 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

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Images

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (AB129106), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (AB129106), expandable thumbnail
  • Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (AB129106), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Rat
Predicted
Not recommended
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal glutathione S transferase Omega 1/p28 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8370(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glutathione S-transferase Omega 1 also known as GSTO1 or p28 is an enzyme that plays an important role in detoxification processes. It belongs to the omega class of glutathione S-transferases and has a molecular mass of approximately 28 kDa. This enzyme is expressed in various human tissues including the liver kidney and brain where it performs important metabolic functions. GSTO1 is not only involved in catalyzing the reduction of hydroxyecdysone metabolites such as ecdysterone (20-hydroxyecdysone) but also participates in modulating cellular responses to oxidative stress.

Biological function summary

GSTO1 is part of a complex network of proteins involved in protecting cells from oxidative damage. This enzyme takes part in antioxidant defense by catalyzing the conjugation of glutathione with harmful compounds facilitating their elimination. As a part of detoxification processes GSTO1 assists in maintaining cellular redox balance. Such activities are essential for several physiological functions including the metabolism of steroid compounds which can relate to ecdysteroid steroid activity. Its activity influences not only metabolic stability but also cell survival and integrity.

Pathways

GSTO1 functions in significant signaling pathways particularly in glutathione metabolism and stress response pathways. It interacts with proteins such as glutaredoxins and thioredoxins playing a role in regulating redox-sensitive signaling. Through its involvement in glutathione pathways GSTO1 helps mitigate damage from reactive oxygen species (ROS) and supports cellular repair mechanisms. Its actions link it with the broader antioxidant networks that maintain cellular homeostasis and modulate stress-induced pathways.

Associated diseases and disorders

GSTO1 has connections to neurological and inflammatory conditions. For instance alterations in GSTO1 expression have been associated with Alzheimer's disease where oxidative stress plays a pivotal role. Similarly it seems implicated in inflammatory disorders due to its capacity to modulate inflammatory responses. Dysfunctional GSTO1 activity can result in an imbalance in oxidative stress pathways linking it with disease-related proteins like amyloid beta in neurodegenerative diseases and inflammatory cytokines in immune-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106), expandable thumbnail

    Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106)

    Lanes 1-4: Merged signal (red and green). Green - ab129106 observed at 28 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab129106 Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] was shown to specifically react with glutathione S transferase Omega 1/p28 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human GSTO1 (glutathione S transferase Omega 1/p28) knockout HEK-293T cell line ab266277 (knockout cell lysate Human GSTO1 (glutathione S transferase Omega 1/p28) knockout HEK-293T cell lysate ab257982) was used. Wild-type and glutathione S transferase Omega 1/p28 knockout samples were subjected to SDS-PAGE. ab129106 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: GSTO1 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human GSTO1 (glutathione S transferase Omega 1/p28) knockout HEK-293T cell line (Human GSTO1 (glutathione S transferase Omega 1/p28) knockout HEK-293T cell line ab266277)

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: BxPC-3 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106)

    ab129106, at 1/100 dilution, staining glutathione S transferase Omega 1/p28 in Paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106), expandable thumbnail

    Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106)

    All lanes: Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: BxPC3 lysate at 10 µg

    Lane 3: HepG2 lysate at 10 µg

    Lane 4: HeLa lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 28 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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