Rabbit Recombinant Monoclonal glutathione S transferase Omega 1/p28 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
IHC-P | IP | Flow Cyt | WB | |
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Human | Tested | Not recommended | Not recommended | Tested |
Rat | Predicted | Not recommended | Not recommended | Predicted |
Species | Dilution info | Notes |
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Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
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Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
GSTTLP28, GSTO1, Glutathione S-transferase omega-1, GSTO-1, Glutathione S-transferase omega 1-1, Glutathione-dependent dehydroascorbate reductase, Monomethylarsonic acid reductase, S-(Phenacyl)glutathione reductase, GSTO 1-1, MMA(V) reductase, SPG-R
Rabbit Recombinant Monoclonal glutathione S transferase Omega 1/p28 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Glutathione S-transferase Omega 1 also known as GSTO1 or p28 is an enzyme that plays an important role in detoxification processes. It belongs to the omega class of glutathione S-transferases and has a molecular mass of approximately 28 kDa. This enzyme is expressed in various human tissues including the liver kidney and brain where it performs important metabolic functions. GSTO1 is not only involved in catalyzing the reduction of hydroxyecdysone metabolites such as ecdysterone (20-hydroxyecdysone) but also participates in modulating cellular responses to oxidative stress.
GSTO1 is part of a complex network of proteins involved in protecting cells from oxidative damage. This enzyme takes part in antioxidant defense by catalyzing the conjugation of glutathione with harmful compounds facilitating their elimination. As a part of detoxification processes GSTO1 assists in maintaining cellular redox balance. Such activities are essential for several physiological functions including the metabolism of steroid compounds which can relate to ecdysteroid steroid activity. Its activity influences not only metabolic stability but also cell survival and integrity.
GSTO1 functions in significant signaling pathways particularly in glutathione metabolism and stress response pathways. It interacts with proteins such as glutaredoxins and thioredoxins playing a role in regulating redox-sensitive signaling. Through its involvement in glutathione pathways GSTO1 helps mitigate damage from reactive oxygen species (ROS) and supports cellular repair mechanisms. Its actions link it with the broader antioxidant networks that maintain cellular homeostasis and modulate stress-induced pathways.
GSTO1 has connections to neurological and inflammatory conditions. For instance alterations in GSTO1 expression have been associated with Alzheimer's disease where oxidative stress plays a pivotal role. Similarly it seems implicated in inflammatory disorders due to its capacity to modulate inflammatory responses. Dysfunctional GSTO1 activity can result in an imbalance in oxidative stress pathways linking it with disease-related proteins like amyloid beta in neurodegenerative diseases and inflammatory cytokines in immune-related disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab129106 Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] was shown to specifically react with glutathione S transferase Omega 1/p28 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human GSTO1 (glutathione S transferase Omega 1/p28) knockout HEK-293T cell line ab266277 (knockout cell lysate Human GSTO1 (glutathione S transferase Omega 1/p28) knockout HEK-293T cell lysate ab257982) was used. Wild-type and glutathione S transferase Omega 1/p28 knockout samples were subjected to SDS-PAGE. ab129106 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: GSTO1 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human GSTO1 (glutathione S transferase Omega 1/p28) knockout HEK-293T cell line (Human GSTO1 (glutathione S transferase Omega 1/p28) knockout HEK-293T cell line ab266277)
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: BxPC-3 cell lysate at 20 µg
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
ab129106, at 1/100 dilution, staining glutathione S transferase Omega 1/p28 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes: Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution
Lane 1: Human fetal liver lysate at 10 µg
Lane 2: BxPC3 lysate at 10 µg
Lane 3: HepG2 lysate at 10 µg
Lane 4: HeLa lysate at 10 µg
All lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
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