AB248304

Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal glutathione S transferase Omega 1/p28 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

GSTTLP28, GSTO1, Glutathione S-transferase omega-1, GSTO-1, Glutathione S-transferase omega 1-1, Glutathione-dependent dehydroascorbate reductase, Monomethylarsonic acid reductase, S-(Phenacyl)glutathione reductase, GSTO 1-1, MMA(V) reductase, SPG-R

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (AB248304)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (AB248304)

This data was developed using ab129106, the same antibody clone in a different buffer formulation.

ab129106, at 1/100 dilution, staining glutathione S transferase Omega 1/p28 in Paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (AB248304)

This data was developed using ab129106, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab129106 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.

ab129106 Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] was shown to specifically react with glutathione S transferase Omega 1/p28 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266277 (knockout cell lysate ab257982) was used. Wild-type and glutathione S transferase Omega 1/p28 knockout samples were subjected to SDS-PAGE. ab129106 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (<a href='/en-us/products/primary-antibodies/glutathione-s-transferase-omega-1-p28-antibody-epr8370b-ab129106'>ab129106</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

GSTO1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human GSTO1 (glutathione S transferase Omega 1/p28) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-gsto1-glutathione-s-transferase-omega-1-p28-knockout-hek-293t-cell-line-ab266277'>ab266277</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

BxPC-3 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Unknown

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (AB248304)

This data was developed using ab129106, the same antibody clone in a different buffer formulation.

All lanes:

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

BxPC3 lysate at 10 µg

Lane 3:

HepG2 lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 28 kDa

false

Unconjugated

Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8370(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248304 is the carrier-free version of ab129106.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione S-transferase Omega 1 also known as GSTO1 or p28 is an enzyme that plays an important role in detoxification processes. It belongs to the omega class of glutathione S-transferases and has a molecular mass of approximately 28 kDa. This enzyme is expressed in various human tissues including the liver kidney and brain where it performs important metabolic functions. GSTO1 is not only involved in catalyzing the reduction of hydroxyecdysone metabolites such as ecdysterone (20-hydroxyecdysone) but also participates in modulating cellular responses to oxidative stress.

Biological function summary

GSTO1 is part of a complex network of proteins involved in protecting cells from oxidative damage. This enzyme takes part in antioxidant defense by catalyzing the conjugation of glutathione with harmful compounds facilitating their elimination. As a part of detoxification processes GSTO1 assists in maintaining cellular redox balance. Such activities are essential for several physiological functions including the metabolism of steroid compounds which can relate to ecdysteroid steroid activity. Its activity influences not only metabolic stability but also cell survival and integrity.

Pathways

GSTO1 functions in significant signaling pathways particularly in glutathione metabolism and stress response pathways. It interacts with proteins such as glutaredoxins and thioredoxins playing a role in regulating redox-sensitive signaling. Through its involvement in glutathione pathways GSTO1 helps mitigate damage from reactive oxygen species (ROS) and supports cellular repair mechanisms. Its actions link it with the broader antioxidant networks that maintain cellular homeostasis and modulate stress-induced pathways.

GSTO1 has connections to neurological and inflammatory conditions. For instance alterations in GSTO1 expression have been associated with Alzheimer's disease where oxidative stress plays a pivotal role. Similarly it seems implicated in inflammatory disorders due to its capacity to modulate inflammatory responses. Dysfunctional GSTO1 activity can result in an imbalance in oxidative stress pathways linking it with disease-related proteins like amyloid beta in neurodegenerative diseases and inflammatory cytokines in immune-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Also has glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.

See full target information GSTO1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (AB248304)

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (AB248304)

Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (AB248304)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise