Anti-Glutathione Synthetase antibody [EPR6562] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] - BSA and Azide free (AB248015)

This data was developed using ab124811, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling Glutathione Synthetase with Purified ab124811 at 1 : 8000 (0.11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6562] - BSA and Azide free (AB248015)

This data was developed using ab124811, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling Glutathione Synthetase with Purified ab124811 at 1 : 8000 (0.11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• WB

Lab

Western blot - Anti-Glutathione Synthetase antibody [EPR6562] - BSA and Azide free (AB248015)

This data was developed using the same antibody clone in a different buffer formulation (ab124811).

Lanes 1- 4 : Merged signal (red and green). Green - ab124811 observed at 52 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab124811 was shown to react with GSS in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266342 (knockout cell lysate ab257460) was used. Wild-type HEK-293T and GSS knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab124811 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (<a href='/en-us/products/primary-antibodies/glutathione-synthetase-antibody-epr6562-ab124811'>ab124811</a>) at 1/10000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

GSS knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human GSS (Glutathione Synthetase) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-gss-glutathione-synthetase-knockout-hek-293t-cell-line-ab266342'>ab266342</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

• WB

Unknown

Western blot - Anti-Glutathione Synthetase antibody [EPR6562] - BSA and Azide free (AB248015)

All lanes:

Western blot - Anti-Glutathione Synthetase antibody [EPR6562] (<a href='/en-us/products/primary-antibodies/glutathione-synthetase-antibody-epr6562-ab124811'>ab124811</a>) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 52 kDa

false