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AB279369

Anti-GLYAT/GAT antibody [OTI4D1]

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(1 Publication)

Mouse Monoclonal GLYAT/GAT antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GLYAT.

View Alternative Names

ACGNAT, CAT, GAT, GLYAT, Glycine N-acyltransferase, Acyl-CoA:glycine N-acyltransferase, Aralkyl acyl-CoA N-acyltransferase, Aralkyl acyl-CoA:amino acid N-acyltransferase, Benzoyl-coenzyme A:glycine N-acyltransferase, Glycine N-benzoyltransferase, HRP-1(CLP), AAc

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYAT/GAT antibody [OTI4D1] (AB279369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYAT/GAT antibody [OTI4D1] (AB279369)

Paraffin embedded human liver tissue stained for GLYAT using ab279369 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYAT/GAT antibody [OTI4D1] (AB279369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYAT/GAT antibody [OTI4D1] (AB279369)

Paraffin embedded Human Kidney tissue stained for GLYAT using ab279369 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYAT/GAT antibody [OTI4D1] (AB279369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYAT/GAT antibody [OTI4D1] (AB279369)

Paraffin embedded carcinoma of Human liver tissue stained for GLYAT using ab279369 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3 minutes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4D1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GLYAT.

Q6IB77

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLYAT also known as glycine-N-acyltransferase or GAT is an enzyme involved in glycine conjugation. This enzyme has a molecular mass of approximately 33 kDa. GLYAT is mainly expressed in the liver and is found in mitochondria. Its mechanical role involves transferring acyl groups from acyl-CoA to glycine forming acyl-glycine conjugates. These conjugates facilitate the excretion of harmful metabolites from the body contributing to detoxification processes.
Biological function summary

GLYAT functions as part of a detoxification pathway. By conjugating glycine to various acyl-CoA substrates it assists in the removal of xenobiotics and other acyl-containing toxins. This action is particularly important for the prevention of acidosis and maintenance of metabolic homeostasis. The enzyme does not typically form part of a larger complex but works alongside other enzymes involved in amino acid metabolism.

Pathways

GLYAT plays an essential role in the glycine conjugation pathway. This pathway is important for detoxifying benzoic acid and other aromatic carboxylic acids. Related proteins include various CoA synthetases which provide the acyl-CoA substrates needed for GLYAT activity. The glycine conjugation pathway intersects with central metabolic networks like amino acid metabolism linking GLYAT functionally to both catabolic and anabolic processes.

Impaired GLYAT function may relate to metabolic issues like hyperglycinemia and certain cases of congenital metabolic disorders of detoxification. Its activity is also relevant in conditions characterized by an accumulation of organic acids. Dysfunctional GLYAT can lead to toxic metabolite build-up emphasizing its importance in maintaining metabolic health. Disorders connected to GLYAT often involve other enzymes in detoxification pathways such as those enzymes implicated in amino acid degradation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid.
See full target information GLYAT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 17:61 PubMed38486335

2024

Unraveling the molecular mechanisms of lymph node metastasis in ovarian cancer: focus on MEOX1.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Li,Yihua Sun,Xiuling Zhi,Yating Sun,Zulimire Abudousalamu,Qianhan Lin,Bin Li,Liangqing Yao,Mo Chen
View all publications
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Product promise

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