Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on human kidney tubules (PMID : 34098990). The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human spleen. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on human liver. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Glycerol 3 Phosphate Dehydrogenase was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab313871 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313871 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 2 : ab313871 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313871 in HepG2 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (<a href='/en-us/products/primary-antibodies/glycerol-3-phosphate-dehydrogenase-antibody-epr25996-4-ab313871'>ab313871</a>) at 1/30 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 15s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on rat kidney tubules. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on mouse kidney tubules (PMID : 6782104). The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on rat liver. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse spleen. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on mouse liver. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Low expression : MCF7 and SK-BR-3.

In Western blot, Anti-GAPDH antibody [EPR16891] (ab181602) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (<a href='/en-us/products/primary-antibodies/glycerol-3-phosphate-dehydrogenase-antibody-epr25996-4-ab313871'>ab313871</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human skeletal muscle cytoplasma tissue lysate at 20 µg

Lane 5:

human liver tissue lysate at 20 µg

Lane 6:

Mouse liver tissue lysate at 20 µg

Lane 7:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free (AB313872)

This data was developed using ab313871, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : 1 seconds

All lanes:

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (<a href='/en-us/products/primary-antibodies/glycerol-3-phosphate-dehydrogenase-antibody-epr25996-4-ab313871'>ab313871</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Lane 6:

Rat skeletal muscle tissue lysate at 20 µg

Lane 7:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 35 kDa

false

Exposure time: 1s