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AB193521

Anti-Glycerol kinase antibody

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(1 Publication)

Rabbit Polyclonal Glycerol kinase antibody. Suitable for WB and reacts with Escherichia coli samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Escherichia coli K-12 glpK.

View Alternative Names

b3926, JW3897, glpK, Glycerol kinase, ATP:glycerol 3-phosphotransferase, Glycerokinase, GK

2 Images
Western blot - Anti-Glycerol kinase antibody (AB193521)
  • WB

Supplier Data

Western blot - Anti-Glycerol kinase antibody (AB193521)

All lanes:

Western blot - Anti-Glycerol kinase antibody (ab193521) at 2 µg/mL

All lanes:

DH5a whole cell lysate

Secondary

All lanes:

Polyclonal goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 54 kDa,61 kDa

Observed band size: 62 kDa

false

Western blot - Anti-Glycerol kinase antibody (AB193521)
  • WB

Supplier Data

Western blot - Anti-Glycerol kinase antibody (AB193521)

All lanes:

Western blot - Anti-Glycerol kinase antibody (ab193521) at 3 µg/mL

Lane 1:

Rosseta bacteria lysate at 80 µg

Lane 2:

Rosseta bacteria lysate at 40 µg

Lane 3:

Rosseta bacteria lysate at 20 µg

Lane 4:

Rosseta bacteria lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/5000 dilution

Predicted band size: 61 kDa

Observed band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Escherichia coli

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Escherichia coli K-12 glpK.

P0A6F3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
>95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol kinase also known as GK or Gyk acts as a catalyst in the phosphorylation of glycerol to glycerol-3-phosphate. This enzymatic activity is important for the utilization of glycerol in metabolic processes. Glycerol kinase is expressed in tissues such as liver kidney and adipose tissue. Its molecular weight varies depending on the species but in humans it is around 60 kDa when analyzed using methods like glycerol SDS PAGE. In human cells like HEK293T reliable detection of glycerol kinase is possible due to its considerable expression.
Biological function summary

The main role of glycerol kinase relates to its function in lipid metabolism and energy homeostasis. It exists as a part of a biochemical pathway but does not typically form a complex with other proteins. Glycerol kinase enables cells to utilize glycerol as an energy source by converting it into glycerol-3-phosphate which feeds into further metabolic pathways. This conversion is essential for the balance of energy supply and the production of triglycerides and phospholipids.

Pathways

Metabolism and energy production heavily rely on the function of glycerol kinase. This enzyme acts within the glycerolipid metabolism and gluconeogenesis pathways. In these pathways it is connected to other enzymes like glycerol-3-phosphate dehydrogenase which further processes the glycerol-3-phosphate produced by glycerol kinase. The enzyme's availability affects the rate of these pathways and in turn influences lipid and glucose metabolism in the body.

Glycerol kinase deficiency leads to complex metabolic syndromes such as hyperglycerolemia and congenital adrenal hyperplasia. Hyperglycerolemia results from excess glycerol in the blood due to impaired conversion by the enzyme. In congenital adrenal hyperplasia alterations in metabolic function relate to glycerol kinase's impact on stress response and energy balance. Disorders related to glycerol kinase sometimes involve interactions with other proteins and enzymes involved in broader metabolic processes affecting overall metabolism and physiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. It also catalyzes the phosphorylation of dihydroxyacetone, L-glyceraldehyde and D-glyceraldehyde. It uses only ATP.
See full target information glpK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Microbiome 11:41 PubMed36869365

2023

Maternal effects drive intestinal development beginning in the embryonic period on the basis of maternal immune and microbial transfer in chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Haizhou Gong,Taiping Wang,Min Wu,Qianran Chu,Hainan Lan,Wuying Lang,Lingyu Zhu,Yang Song,Yujie Zhou,Qiongyi Wen,Jing Yu,Baolin Wang,Xin Zheng
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