AB228615

Anti-Glycerol kinase antibody

Name: Anti-Glycerol kinase antibody (ab228615) | Abcam
Brand: Abcam Limited
SKU: AB228615
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Glycerol kinase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glycerol kinase.

View Alternative Names

Glycerol kinase, Glycerokinase, ATP:glycerol 3-phosphotransferase, GK

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Glycerol kinase antibody (AB228615)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glycerol kinase antibody (AB228615)

Ice-cold methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Glycerol kinase (green) using ab228615 at 1/500 dilution in ICC/IF.

Nuclear counter stain : Hoechst 33342 (blue).

Supplier Data

Western blot - Anti-Glycerol kinase antibody (AB228615)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Glycerol kinase antibody (ab228615) at 1/1000 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 61 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Glycerol kinase. The exact immunogen used to generate this antibody is proprietary information.

P32189

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol kinase also known as GK or Gyk acts as a catalyst in the phosphorylation of glycerol to glycerol-3-phosphate. This enzymatic activity is important for the utilization of glycerol in metabolic processes. Glycerol kinase is expressed in tissues such as liver kidney and adipose tissue. Its molecular weight varies depending on the species but in humans it is around 60 kDa when analyzed using methods like glycerol SDS PAGE. In human cells like HEK293T reliable detection of glycerol kinase is possible due to its considerable expression.

Biological function summary

The main role of glycerol kinase relates to its function in lipid metabolism and energy homeostasis. It exists as a part of a biochemical pathway but does not typically form a complex with other proteins. Glycerol kinase enables cells to utilize glycerol as an energy source by converting it into glycerol-3-phosphate which feeds into further metabolic pathways. This conversion is essential for the balance of energy supply and the production of triglycerides and phospholipids.

Pathways

Metabolism and energy production heavily rely on the function of glycerol kinase. This enzyme acts within the glycerolipid metabolism and gluconeogenesis pathways. In these pathways it is connected to other enzymes like glycerol-3-phosphate dehydrogenase which further processes the glycerol-3-phosphate produced by glycerol kinase. The enzyme's availability affects the rate of these pathways and in turn influences lipid and glucose metabolism in the body.

Glycerol kinase deficiency leads to complex metabolic syndromes such as hyperglycerolemia and congenital adrenal hyperplasia. Hyperglycerolemia results from excess glycerol in the blood due to impaired conversion by the enzyme. In congenital adrenal hyperplasia alterations in metabolic function relate to glycerol kinase’s impact on stress response and energy balance. Disorders related to glycerol kinase sometimes involve interactions with other proteins and enzymes involved in broader metabolic processes affecting overall metabolism and physiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Kinase that plays a key role in glycerol metabolism, catalyzing its phosphorylation to produce sn-glycerol 3-phosphate. Sn-glycerol 3-phosphate is a crucial intermediate in various metabolic pathways, such as the synthesis of glycerolipids and triglycerides, glycogenesis, glycolysis and gluconeogenesis.

See full target information Glycerol kinase

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401311 PubMed39418169

2024

Glycerol Kinase Drives Hepatic de novo Lipogenesis and Triglyceride Synthesis in Nonalcoholic Fatty Liver by Activating SREBP-1c Transcription, Upregulating DGAT1/2 Expression, and Promoting Glycerol Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyu Ouyang,Shu Zhuo,Mengmei Yang,Tengfei Zhu,Shuting Yu,Yu Li,Hao Ying,Yingying Le

Neurology(R) neuroimmunology & neuroinflammation 10: PubMed37591767

2023

Electrolyte Imbalance in Anti-LGI1 Encephalitis: It Is Not All in Your Head.

Applications

Unspecified application

Species

Unspecified reactive species

Avi Gadoth,Michal Nisnboym,Yifat Alcalay,Asia Zubkov,Idit Schwartz,Doron Schwartz,Marana Abboud,Tamar Rubinek,Ofer Yossepowitch,Talia Weinstein

Biology 10: PubMed34356519

2021

Downregulation of Ribosomal Contents and Kinase Activities Is Associated with the Inhibitive Effect on the Growth of Group B Streptococcus Induced by Placental Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Gao,Yunhui Tang,Xinyi Sun,Qiujing Chen,Yiqian Peng,Catherine Jia-Yun Tsai,Qi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Glycerol kinase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Glycerol kinase antibody (AB228615)

Western blot - Anti-Glycerol kinase antibody (AB228615)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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