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Rabbit Polyclonal Glycine decarboxylase antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLDC aa 50-300.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB232989), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB232989), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB232989), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB232989), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB232989), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human GLDC aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link P23378

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Pig
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

0.2-2 µg/mL

Notes

-

Species

Pig

Dilution info

0.2-2 µg/mL

Notes

-

Species

Human

Dilution info

0.2-2 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

5-20 µg/mL

Notes

-

Expected
Expected

Species

Mouse, Pig

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).

Alternative names

Recommended products

Rabbit Polyclonal Glycine decarboxylase antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLDC aa 50-300.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human GLDC aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link P23378
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glycine decarboxylase also known as P-protein catalyzes the decarboxylation of glycine. This reaction releases carbon dioxide and transfers the remaining methylamine to tetrahydrofolate. Glycine decarboxylase is an essential component of the glycine cleavage system which consists of four proteins: P H T and L-proteins. The molecular mass of glycine decarboxylase is approximately 1000 kDa. This protein is mainly expressed in the mitochondria of liver and kidney cells where it plays an important role in energy metabolism.

Biological function summary

Glycine decarboxylase functions in cellular metabolism and photorespiration. It is part of the glycine cleavage system complex which is responsible for breaking down glycine into CO2 NH3 and a methylene group. The methylene group then enters the folate pool and contributes to various one-carbon metabolism pathways. This process helps regulate cellular levels of glycine and serine both important for nucleotide and protein synthesis as well as maintaining the balance of oxidative stress within cells.

Pathways

Glycine decarboxylase plays an important role in the folate and photorespiratory pathways. In the folate pathway interactions with serine hydroxymethyltransferase allow for the conversion of serine to glycine and the transfer of one-carbon units needed for nucleic acid synthesis. In the photorespiratory pathway its activity helps recycle 2-phosphoglycolate in plant mitochondria minimizing energy loss. The protein dihydrolipoamide dehydrogenase part of the same enzyme complex assists in maintaining the cycle of oxidation and reduction necessary for these functions.

Associated diseases and disorders

Glycine decarboxylase dysfunction associates with nonketotic hyperglycinemia and certain liver diseases. In nonketotic hyperglycinemia defective glycine cleavage leads to excess glycine accumulation causing neurological symptoms such as seizures and developmental delays. Liver diseases linked to abnormal glycine metabolism can arise from impaired liver enzyme activities including those involving glycine decarboxylase. Another related protein AMT (aminomethyltransferase) also contributes to such conditions when mutations affect the glycine cleavage system.

Product promise

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8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989)

    Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Glycine decarboxylase using ab232989 at 30μg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989)

    Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Glycine decarboxylase using ab232989 at 20μg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989)

    Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Glycine decarboxylase using ab232989 at 20μg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for Glycine decarboxylase using ab232989 at 20μg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (ab232989)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for Glycine decarboxylase using ab232989 at 20μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Glycine decarboxylase antibody (ab232989), expandable thumbnail

    Western blot - Anti-Glycine decarboxylase antibody (ab232989)

    All lanes: Western blot - Anti-Glycine decarboxylase antibody (ab232989) at 2 µg/mL

    All lanes: Recombinant human Glycine decarboxylase protein

    Predicted band size: 112 kDa

  • Western blot - Anti-Glycine decarboxylase antibody (ab232989), expandable thumbnail

    Western blot - Anti-Glycine decarboxylase antibody (ab232989)

    All lanes: Western blot - Anti-Glycine decarboxylase antibody (ab232989) at 2 µg/mL

    All lanes: Mouse liver tissue lysate

    Secondary

    All lanes: HRP-Linked Guinea pig Anti-Rabbit Ab at 1/1000 dilution

    Predicted band size: 112 kDa

  • Western blot - Anti-Glycine decarboxylase antibody (ab232989), expandable thumbnail

    Western blot - Anti-Glycine decarboxylase antibody (ab232989)

    All lanes: Western blot - Anti-Glycine decarboxylase antibody (ab232989) at 2 µg/mL

    All lanes: Pig liver tissue lysate

    Secondary

    All lanes: HRP-Linked Guinea pig Anti-Rabbit Ab at 1/1000 dilution

    Predicted band size: 112 kDa

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