Rabbit Polyclonal Glycine decarboxylase antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLDC aa 50-300.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Pig | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.2-2 µg/mL | Notes - |
Species Pig | Dilution info 0.2-2 µg/mL | Notes - |
Species Human | Dilution info 0.2-2 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).
GCSP, GLDC, Glycine cleavage system P protein, Glycine decarboxylase, Glycine dehydrogenase (aminomethyl-transferring)
Rabbit Polyclonal Glycine decarboxylase antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLDC aa 50-300.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS
Liquid
Polyclonal
Affinity purification Immunogen
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Glycine decarboxylase also known as P-protein catalyzes the decarboxylation of glycine. This reaction releases carbon dioxide and transfers the remaining methylamine to tetrahydrofolate. Glycine decarboxylase is an essential component of the glycine cleavage system which consists of four proteins: P H T and L-proteins. The molecular mass of glycine decarboxylase is approximately 1000 kDa. This protein is mainly expressed in the mitochondria of liver and kidney cells where it plays an important role in energy metabolism.
Glycine decarboxylase functions in cellular metabolism and photorespiration. It is part of the glycine cleavage system complex which is responsible for breaking down glycine into CO2 NH3 and a methylene group. The methylene group then enters the folate pool and contributes to various one-carbon metabolism pathways. This process helps regulate cellular levels of glycine and serine both important for nucleotide and protein synthesis as well as maintaining the balance of oxidative stress within cells.
Glycine decarboxylase plays an important role in the folate and photorespiratory pathways. In the folate pathway interactions with serine hydroxymethyltransferase allow for the conversion of serine to glycine and the transfer of one-carbon units needed for nucleic acid synthesis. In the photorespiratory pathway its activity helps recycle 2-phosphoglycolate in plant mitochondria minimizing energy loss. The protein dihydrolipoamide dehydrogenase part of the same enzyme complex assists in maintaining the cycle of oxidation and reduction necessary for these functions.
Glycine decarboxylase dysfunction associates with nonketotic hyperglycinemia and certain liver diseases. In nonketotic hyperglycinemia defective glycine cleavage leads to excess glycine accumulation causing neurological symptoms such as seizures and developmental delays. Liver diseases linked to abnormal glycine metabolism can arise from impaired liver enzyme activities including those involving glycine decarboxylase. Another related protein AMT (aminomethyltransferase) also contributes to such conditions when mutations affect the glycine cleavage system.
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Glycine decarboxylase using ab232989 at 30μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Glycine decarboxylase using ab232989 at 20μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Glycine decarboxylase using ab232989 at 20μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Glycine decarboxylase using ab232989 at 20μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach tissue stained for Glycine decarboxylase using ab232989 at 20μg/ml in immunohistochemical analysis. DAB staining.
All lanes: Western blot - Anti-Glycine decarboxylase antibody (ab232989) at 2 µg/mL
All lanes: Recombinant human Glycine decarboxylase protein
Predicted band size: 112 kDa
All lanes: Western blot - Anti-Glycine decarboxylase antibody (ab232989) at 2 µg/mL
All lanes: Mouse liver tissue lysate
All lanes: HRP-Linked Guinea pig Anti-Rabbit Ab at 1/1000 dilution
Predicted band size: 112 kDa
All lanes: Western blot - Anti-Glycine decarboxylase antibody (ab232989) at 2 µg/mL
All lanes: Pig liver tissue lysate
All lanes: HRP-Linked Guinea pig Anti-Rabbit Ab at 1/1000 dilution
Predicted band size: 112 kDa
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