Rabbit Polyclonal Glycine decarboxylase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLDC aa 450-700.
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- Frontiers in immunology 13:10333672022GLDC mitigated by miR-30e regulates cell proliferation and tumor immune infiltration in TNBC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuaying Xie,Tingting Yan,Xinxin Lu,Yueyao Du,Shuguang Xu,Yu Kong,Liangjie Yu,Jian Sun,Liheng Zhou,Jun MaPubMed 36275705
- EMBO molecular medicine 11:2018Identification and characterization of GLDC as host susceptibility gene to severe influenza.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zhou,Dong Wang,Bosco Ho-Yin Wong,Cun Li,Vincent Kwok-Man Poon,Lei Wen,Xiaoyu Zhao,Man Chun Chiu,Xiaojuan Liu,Ziwei Ye,Shuofeng Yuan,Kong-Hung Sze,Jasper Fuk-Woo Chan,Hin Chu,Kelvin Kai-Wang To,Kwok Yung YuenPubMed 30498026
- Biochemical pharmacology 151:245-2512018Astrocytes in primary cultures express serine racemase, synthesize d-serine and acquire A1 reactive astrocyte features.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuyan Li et. al.PubMed 29305854
- Annals of neurology 78:606-182015Biochemical and molecular predictors for prognosis in nonketotic hyperglycinemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael A Swanson,Curtis R Coughlin,Gunter H Scharer,Heather J Szerlong,Kendra J Bjoraker,Elaine B Spector,Geralyn Creadon-Swindell,Vincent Mahieu,Gert Matthijs,Julia B Hennermann,Derek A Applegarth,Jennifer R Toone,Suhong Tong,Kristina Williams,Johan L K Van HovePubMed 26179960
- PloS one 9:e1010042014Differential expression of enzymes associated with serine/glycine metabolism in different breast cancer subtypes.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GLDC aa 450-700. The exact immunogen used to generate this antibody is proprietary information. Database link P23378
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).
Alternative names
GCSP, GLDC, Glycine cleavage system P protein, Glycine decarboxylase, Glycine dehydrogenase (aminomethyl-transferring)
Recommended products
Rabbit Polyclonal Glycine decarboxylase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLDC aa 450-700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GLDC aa 450-700. The exact immunogen used to generate this antibody is proprietary information. Database link P23378
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glycine decarboxylase also known as P-protein catalyzes the decarboxylation of glycine. This reaction releases carbon dioxide and transfers the remaining methylamine to tetrahydrofolate. Glycine decarboxylase is an essential component of the glycine cleavage system which consists of four proteins: P H T and L-proteins. The molecular mass of glycine decarboxylase is approximately 1000 kDa. This protein is mainly expressed in the mitochondria of liver and kidney cells where it plays an important role in energy metabolism.
- Biological function summary
Glycine decarboxylase functions in cellular metabolism and photorespiration. It is part of the glycine cleavage system complex which is responsible for breaking down glycine into CO2 NH3 and a methylene group. The methylene group then enters the folate pool and contributes to various one-carbon metabolism pathways. This process helps regulate cellular levels of glycine and serine both important for nucleotide and protein synthesis as well as maintaining the balance of oxidative stress within cells.
- Pathways
Glycine decarboxylase plays an important role in the folate and photorespiratory pathways. In the folate pathway interactions with serine hydroxymethyltransferase allow for the conversion of serine to glycine and the transfer of one-carbon units needed for nucleic acid synthesis. In the photorespiratory pathway its activity helps recycle 2-phosphoglycolate in plant mitochondria minimizing energy loss. The protein dihydrolipoamide dehydrogenase part of the same enzyme complex assists in maintaining the cycle of oxidation and reduction necessary for these functions.
- Associated diseases and disorders
Glycine decarboxylase dysfunction associates with nonketotic hyperglycinemia and certain liver diseases. In nonketotic hyperglycinemia defective glycine cleavage leads to excess glycine accumulation causing neurological symptoms such as seizures and developmental delays. Liver diseases linked to abnormal glycine metabolism can arise from impaired liver enzyme activities including those involving glycine decarboxylase. Another related protein AMT (aminomethyltransferase) also contributes to such conditions when mutations affect the glycine cleavage system.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Glycine decarboxylase antibody (ab97625)
5% SDS-PAGE
All lanes: Western blot - Anti-Glycine decarboxylase antibody (ab97625) at 1/500 dilution
Lane 1: HeLa whole cell lysate at 30 µg
Lane 2: HepG2 whole cell lysate at 30 µg
Predicted band size: 112 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Glycine decarboxylase antibody (ab97625)
ab97625, at a 1/200 dilution, staining Glycine decarboxylase in methanol fixed HeLa cells by Immunofluorescence analysis. Lower image was co-stained with Hoechst 33342.
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