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AB97625

Anti-Glycine decarboxylase antibody

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(5 Publications)

Rabbit Polyclonal Glycine decarboxylase antibody. Suitable for IP, IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLDC aa 450-700.

View Alternative Names

GCSP, GLDC, Glycine cleavage system P protein, Glycine decarboxylase, Glycine dehydrogenase (aminomethyl-transferring)

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Glycine decarboxylase antibody (AB97625)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glycine decarboxylase antibody (AB97625)

Immunofluorescence analysis of Hep G2 cells fixed in ice cold MeOH for 5 min labelling Glycine dehydrogenase protein at mitochondria using ab97625 at a 1/400 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Immunoprecipitation - Anti-Glycine decarboxylase antibody (AB97625)
  • IP

Supplier Data

Immunoprecipitation - Anti-Glycine decarboxylase antibody (AB97625)

Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 µg of ab97625. Western blot analysis was performed using ab97625.

All lanes:

Immunoprecipitation - Anti-Glycine decarboxylase antibody (ab97625) at 5 µg

Lane 1:

HepG2 whole cell extract

Lane 2:

HepG2 whole cell extract with Control IgG

Lane 3:

HepG2 whole cell extract with ab97625

Secondary

All lanes:

EasyBlot anti Rabbit IgG

Predicted band size: 113 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB97625)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB97625)

Immunohistochemical analysis of paraffin embedded mouse liver tissue stained for Glycine dehydrogenase protein at mitochondria with ab97625 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Glycine decarboxylase antibody (AB97625)
  • WB

Supplier Data

Western blot - Anti-Glycine decarboxylase antibody (AB97625)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Glycine decarboxylase antibody (ab97625) at 1/1000 dilution

All lanes:

HepG2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human GLDC aa 450-700. The exact immunogen used to generate this antibody is proprietary information.

P23378

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycine decarboxylase also known as P-protein catalyzes the decarboxylation of glycine. This reaction releases carbon dioxide and transfers the remaining methylamine to tetrahydrofolate. Glycine decarboxylase is an essential component of the glycine cleavage system which consists of four proteins: P H T and L-proteins. The molecular mass of glycine decarboxylase is approximately 1000 kDa. This protein is mainly expressed in the mitochondria of liver and kidney cells where it plays an important role in energy metabolism.
Biological function summary

Glycine decarboxylase functions in cellular metabolism and photorespiration. It is part of the glycine cleavage system complex which is responsible for breaking down glycine into CO2 NH3 and a methylene group. The methylene group then enters the folate pool and contributes to various one-carbon metabolism pathways. This process helps regulate cellular levels of glycine and serine both important for nucleotide and protein synthesis as well as maintaining the balance of oxidative stress within cells.

Pathways

Glycine decarboxylase plays an important role in the folate and photorespiratory pathways. In the folate pathway interactions with serine hydroxymethyltransferase allow for the conversion of serine to glycine and the transfer of one-carbon units needed for nucleic acid synthesis. In the photorespiratory pathway its activity helps recycle 2-phosphoglycolate in plant mitochondria minimizing energy loss. The protein dihydrolipoamide dehydrogenase part of the same enzyme complex assists in maintaining the cycle of oxidation and reduction necessary for these functions.

Glycine decarboxylase dysfunction associates with nonketotic hyperglycinemia and certain liver diseases. In nonketotic hyperglycinemia defective glycine cleavage leads to excess glycine accumulation causing neurological symptoms such as seizures and developmental delays. Liver diseases linked to abnormal glycine metabolism can arise from impaired liver enzyme activities including those involving glycine decarboxylase. Another related protein AMT (aminomethyltransferase) also contributes to such conditions when mutations affect the glycine cleavage system.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).
See full target information GLDC
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:1033367 PubMed36275705

2022

GLDC mitigated by miR-30e regulates cell proliferation and tumor immune infiltration in TNBC.

Applications

Unspecified application

Species

Unspecified reactive species

Huaying Xie,Tingting Yan,Xinxin Lu,Yueyao Du,Shuguang Xu,Yu Kong,Liangjie Yu,Jian Sun,Liheng Zhou,Jun Ma

EMBO molecular medicine 11: PubMed30498026

2018

Identification and characterization of GLDC as host susceptibility gene to severe influenza.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhou,Dong Wang,Bosco Ho-Yin Wong,Cun Li,Vincent Kwok-Man Poon,Lei Wen,Xiaoyu Zhao,Man Chun Chiu,Xiaojuan Liu,Ziwei Ye,Shuofeng Yuan,Kong-Hung Sze,Jasper Fuk-Woo Chan,Hin Chu,Kelvin Kai-Wang To,Kwok Yung Yuen

Biochemical pharmacology 151:245-251 PubMed29305854

2018

Astrocytes in primary cultures express serine racemase, synthesize d-serine and acquire A1 reactive astrocyte features.

Applications

Unspecified application

Species

Unspecified reactive species

Suyan Li,Yota Uno,Uwe Rudolph,Johanna Cobb,Jing Liu,Thea Anderson,Deborah Levy,Darrick T Balu,Joseph T Coyle

Annals of neurology 78:606-18 PubMed26179960

2015

Biochemical and molecular predictors for prognosis in nonketotic hyperglycinemia.

Applications

Unspecified application

Species

Unspecified reactive species

Michael A Swanson,Curtis R Coughlin,Gunter H Scharer,Heather J Szerlong,Kendra J Bjoraker,Elaine B Spector,Geralyn Creadon-Swindell,Vincent Mahieu,Gert Matthijs,Julia B Hennermann,Derek A Applegarth,Jennifer R Toone,Suhong Tong,Kristina Williams,Johan L K Van Hove

PloS one 9:e101004 PubMed24979213

2014

Differential expression of enzymes associated with serine/glycine metabolism in different breast cancer subtypes.

Applications

WB

Species

Human

Sang Kyum Kim,Woo Hee Jung,Ja Seung Koo
View all publications
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