Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (AB2479)

Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue : Human Prostate Fixation : formalin-fixed, paraffin-embedded tissue Antigen retrieval : heat-induced Primary antibody(ab2479) : 5 μg/ml Staining : antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

• WB

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Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (AB2479)

Rabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.

Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.

A 4-20% Criterion gel for SDS-PAGE was used.

All lanes:

Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479) at 1/1000 dilution

Lanes 1 and 5:

mock treated mouse cardiac myocyte lysate at 12 µL

Lane 2:

insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µL

Lane 3:

insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µL

Lane 4:

insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µL

Lane 6:

CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µL

Lane 7:

CLA treated (20 nM, 45min) mouse cardiac myocyte lysate

Lane 8:

CLA treated (100 nM, 45min) mouse cardiac myocyte lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab7090'>ab7090</a>) at 1/5000 dilution

Predicted band size: 84 kDa

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