AB247514

Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Glycogen synthase 1/GYS1 phospho S641 antibody. Carrier free. Suitable for Dot, WB, IHC-P and reacts with Synthetic peptide, Mouse, Human samples.

View Alternative Names

GYS, GYS1, Glycogen synthase 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (AB247514)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (AB247514)

This data was developed using ab81230, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Human liver tissue, using 1/100 ab81230.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (AB247514)

This data was developed using ab81230, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (<a href='/en-us/products/primary-antibodies/glycogen-synthase-1-gys1-phospho-s641-antibody-ep852y-ab81230'>ab81230</a>) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with insulin at 20 ug/mL for 15 minutes. Whole cell lysates at 15 µg

Lane 2:

Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates at 15 µg

Lane 3:

Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates.Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 84 kDa

Observed band size: 85 kDa

false

Exposure time: 1s

Unknown

Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (AB247514)

This data was developed using ab81230, the same antibody clone in a different buffer formulation.

All lanes:

Lane 1:

Mouse muscle tissue lysate, untreated at 10 µg

Lane 2:

Mouse muscle tissue lysate, treated with AP at 10 µg

Secondary

All lanes:

HRP labeled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 84 kDa

Observed band size: 85 kDa

false

Unknown

Dot Blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (AB247514)

This data was developed using ab81230, the same antibody clone in a different buffer formulation.

Dot blot analysis of glycogen synthase 1/GYS1 (phospho S641) phospho peptide (Lane 1) and glycogen synthase 1/GYS1 non-phospho peptide (Lane 2) labeling synthase 1 (phospho S641) with ab81230 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Unconjugated

Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP852Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, Dot, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab247514 is the carrier-free version of ab81230.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogen synthase 1 (GYS1) also known as muscle glycogen synthase catalyzes the synthesis of glycogen from glucose. It adds glucose units from UDP-glucose to the non-reducing ends of glycogen chains facilitating glycogen elongation. The molecular mass of GYS1 is approximately 85 kDa. It is predominantly expressed in skeletal muscle tissue providing energy storage primarily in the form of glycogen. GYS1 is regulated through phosphorylation wherein it undergoes inactive to active conformational changes.

Biological function summary

This enzyme plays a critical role in the energy storage mechanism of muscle tissues. GYS1 functions as a part of the glycogen synthase complex interacting with various glycogen-associated proteins to modulate its activity. These interactions control the enzyme's affinity for its substrate and its susceptibility to regulatory signals like phosphorylation affecting glycogen synthesis efficiency. Through its activity GYS1 ensures a readily available reserve of glucose in muscle tissues for rapid energy production when required.

Pathways

Glycogen synthase 1 is a central component of the glycogen metabolism pathway. It acts alongside other enzymes such as glycogen phosphorylase which catalyzes glycogen breakdown. The balance between GYS1 and glycogen phosphorylase determines the rate of glycogen turnover within cells. GYS1 is further regulated by the insulin signaling pathway where insulin promotes glycogen synthesis by altering GYS1 activity alongside proteins like AKT influencing glucose homeostasis and energy management in tissues.

Dysregulation of GYS1 activity often associates with glycogen storage diseases notably Type 0 where deficiencies in glycogen storage lead to episodes of low blood sugar and reduced exercise capacity. Furthermore aberrations in GYS1 function contribute to insulin resistance and Type 2 diabetes conditions linked with impaired glucose metabolism. Altered GYS1 activity impacts proteins like glycogen phosphorylase affecting overall glycogen metabolism balance and contributing to metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.

See full target information GYS1 phospho S641

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (AB247514)

Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (AB247514)

Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (AB247514)

Dot Blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (AB247514)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise