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AB224552

Anti-Glycogen synthase 2 antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal Glycogen synthase 2 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GYS2 aa 600 to C-terminus.

View Alternative Names

Glycogen synthase 2, GYS2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 2 antibody - C-terminal (AB224552)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 2 antibody - C-terminal (AB224552)

Paraffin-embedded human liver tissue stained for Glycogen synthase 2 using ab224552 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-Glycogen synthase 2 antibody - C-terminal (AB224552)
  • WB

Supplier Data

Western blot - Anti-Glycogen synthase 2 antibody - C-terminal (AB224552)

All lanes:

Western blot - Anti-Glycogen synthase 2 antibody - C-terminal (ab224552) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 81 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GYS2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54840

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogen synthase 2 (GYS2) also known as liver glycogen synthase is an enzyme that plays an important role in glycogen synthesis by converting UDP-glucose into glycogen. It possesses a molecular mass of approximately 79.8 kDa. This enzyme is highly expressed in liver tissues. GYS2 is responsible for the formation of the α-14-glycosidic linkages critical for the elongation of glycogen branches and utilizes UDP-glucose as a substrate.
Biological function summary

Glycogen synthase 2 facilitates the storage of glucose as glycogen in the liver. It is not part of a complex but works alongside other enzymes in the glycogenic process to regulate glucose homeostasis in the body. GYS2 activity gets regulated through allosteric interactions and phosphorylation responding to energy needs of the cell. Its function is instrumental in maintaining proper blood glucose levels especially postprandially when blood glucose rises.

Pathways

Glycogen synthase 2 is integral to the glycogen metabolism pathway. It operates synergistically with other proteins such as glycogen phosphorylase and glycogen-branching enzyme during the synthesis and breakdown of glycogen. GYS2 is also influenced by signaling pathways including the insulin signaling pathway which modulates its activity through phosphorylation. Insulin levels directly impact the enzyme leading to storage or release of glucose based on the body’s energy demands.

There is a strong link between glycogen synthase 2 and glycogen storage disease type 0 (GSD 0). Mutations in the GYS2 gene impair glycogen synthesis resulting in hypoglycemia and hepatomegaly due to insufficient glycogen storage in the liver. Additionally GYS2 alterations may contribute to type 2 diabetes as disturbed glycogen synthesis can affect glucose homeostasis. Insulin-resistant conditions show a decreased activation of GYS2 highlighting its connection with key metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
See full target information GYS2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 9: PubMed32316188

2020

Colocalization of MID1IP1 and c-Myc is Critically Involved in Liver Cancer Growth via Regulation of Ribosomal Protein L5 and L11 and CNOT2.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hoon Jung,Hyo-Jung Lee,Ju-Ha Kim,Deok Yong Sim,Eunji Im,Sinae Kim,Suhwan Chang,Sung-Hoon Kim
View all publications
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