Rabbit Polyclonal Glycogen synthase 2 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GYS2 aa 600 to C-terminus.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GYS2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P54840
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Target data
Function
Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Alternative names
Glycogen synthase 2, GYS2
Recommended products
Rabbit Polyclonal Glycogen synthase 2 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GYS2 aa 600 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GYS2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P54840
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glycogen synthase 2 (GYS2) also known as liver glycogen synthase is an enzyme that plays an important role in glycogen synthesis by converting UDP-glucose into glycogen. It possesses a molecular mass of approximately 79.8 kDa. This enzyme is highly expressed in liver tissues. GYS2 is responsible for the formation of the α-14-glycosidic linkages critical for the elongation of glycogen branches and utilizes UDP-glucose as a substrate.
- Biological function summary
Glycogen synthase 2 facilitates the storage of glucose as glycogen in the liver. It is not part of a complex but works alongside other enzymes in the glycogenic process to regulate glucose homeostasis in the body. GYS2 activity gets regulated through allosteric interactions and phosphorylation responding to energy needs of the cell. Its function is instrumental in maintaining proper blood glucose levels especially postprandially when blood glucose rises.
- Pathways
Glycogen synthase 2 is integral to the glycogen metabolism pathway. It operates synergistically with other proteins such as glycogen phosphorylase and glycogen-branching enzyme during the synthesis and breakdown of glycogen. GYS2 is also influenced by signaling pathways including the insulin signaling pathway which modulates its activity through phosphorylation. Insulin levels directly impact the enzyme leading to storage or release of glucose based on the body’s energy demands.
- Associated diseases and disorders
There is a strong link between glycogen synthase 2 and glycogen storage disease type 0 (GSD 0). Mutations in the GYS2 gene impair glycogen synthesis resulting in hypoglycemia and hepatomegaly due to insufficient glycogen storage in the liver. Additionally GYS2 alterations may contribute to type 2 diabetes as disturbed glycogen synthesis can affect glucose homeostasis. Insulin-resistant conditions show a decreased activation of GYS2 highlighting its connection with key metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Glycogen synthase 2 antibody - C-terminal (ab224552)
All lanes: Western blot - Anti-Glycogen synthase 2 antibody - C-terminal (ab224552) at 1/100 dilution
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG (human brain glioma cell line) cell lysate
Lane 3: Human plasma
Lane 4: Human liver tissue lysate
Lane 5: Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 2 antibody - C-terminal (ab224552)
Paraffin-embedded human liver tissue stained for Glycogen synthase 2 using ab224552 at 1/200 dilution in immunohistochemical analysis.
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