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AB204784

Anti-Glycogenin 2 antibody

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(2 Publications)

Rabbit Polyclonal Glycogenin 2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glycogenin-2 aa 350-500.

View Alternative Names

Glycogenin-2, GN-2, GN2, GYG2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogenin 2 antibody (AB204784)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogenin 2 antibody (AB204784)

Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling Glycogenin 2 with ab204784 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Glycogenin 2 antibody (AB204784)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glycogenin 2 antibody (AB204784)

Immunofluorescent analysis of SK-MEL-30 cells (PFA-fixed/Triton X-100 permeabilized) labeling Glycogenin 2 with ab204784 at 4 μg/ml (green).

Western blot - Anti-Glycogenin 2 antibody (AB204784)
  • WB

Supplier Data

Western blot - Anti-Glycogenin 2 antibody (AB204784)

All lanes:

Western blot - Anti-Glycogenin 2 antibody (ab204784) at 1/500 dilution

Lane 1:

RT-4 cell lysates

Lane 2:

U-251 MG cell lysates

Predicted band size: 55 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Glycogenin-2 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

O15488

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogenin 2 sometimes referred to as GN2 is a protein involved in the initiation of glycogen synthesis. It acts as the primer initiating the polymerization process by attaching glucose residues to itself to form the core of glycogen particles. Glycogenin 2 has a molecular mass of approximately 66 kDa. It is expressed in many tissues particularly in liver and muscle tissues where glycogen synthesis is essential for glucose storage.
Biological function summary

As a self-glucosylating enzyme glycogenin 2 plays an important role in glycogen particle formation. It exists as a homodimer a reflection of its ability to self-prime and extend glucose polymers. Through this activity it helps maintain the balance of energy supply in cells a function critical in tissues with high energy demands. Despite its catalytic role it interacts with other enzymes such as glycogen synthase to carry out its biological activities effectively.

Pathways

The enzymatic activity of glycogenin 2 is central to glycogen biosynthesis. It acts upstream of the glycogen synthesis pathway providing the necessary primer for glycogen synthase to extend the glucose chain. This pathway is vital for glucose storage regulation in organisms. Glycogenin 2 works alongside proteins like UDP-glucose pyrophosphorylase which supplies the active glucose moiety for glycogen chain elongation.

Glycogenin 2's function is important in glycogen storage diseases like glycogen storage disease type 0. Its malfunction leads to improper glycogen synthesis resulting in energy storage deficiencies. In some hepatic conditions altered glycogenin 2 activity has been observed playing a role in the irregular accumulation of glycogen. Additionally its interaction with glycogen synthase can complicate this scenario as the disruption of their interaction can inhibit normal glycogen deposition affecting metabolic health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycogenin participates in the glycogen biosynthetic process along with glycogen synthase and glycogen branching enzyme. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.
See full target information Glycogenin-2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:22028 PubMed39322716

2024

Autologous micrografting improves regeneration of tissue-engineered urinary conduits in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Nikolai Juul,Mahboobeh Amoushahi,Oliver Willacy,Micki Ji,Chiara Villa,Fatemeh Ajalloueian,Clara Chamorro,Magdalena Fossum

iScience 24:102724 PubMed34337357

2021

Microscopic image-based covariation network analysis for actin scaffold-modified insulin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiyuki Noguchi,Fumi Kano,Nobuhiko Maiya,Chisako Iwamoto,Shoko Yamasaki,Yosuke Otsubo,Daiki Nakatsu,Rina Kunishige,Masayuki Murata
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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