Mouse Monoclonal Glycophorin A antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GYPA.
IgG1
Mouse
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
IHC-P | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-0.50000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Select an associated product type
Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane (PubMed:35835865). Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane.(Microbial infection) Appears to be a receptor for Hepatitis A virus (HAV).(Microbial infection) Receptor for P.falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans.
GPA, MNS, GPA, GYPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha
Mouse Monoclonal Glycophorin A antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GYPA.
IgG1
Mouse
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
Clone GYPA/280
Affinity purification Protein A/G
kappa
purified from Bioreactor Concentrates
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Glycophorin A also known as CD235a is a major glycoprotein of red blood cell membranes with a mass around 31 kDa. It is importantly expressed on the surface of erythrocytes. Glycophorin A plays an important role in maintaining cell membrane integrity and is associated with various antigenic sites which are important for compatibility testing. Researchers often detect Glycophorin A using antibodies such as Glycophorin A APC or Glycophorin A FITC in flow cytometry studies due to its distinct expression on red blood cells.
Glycophorin A interacts with other membrane proteins to form part of the membrane's skeletal protein network. This network stabilizes the erythrocyte structure and contributes to its flexibility essential for passing through narrow capillaries. Glycophorin A does not form part of any large multiprotein complex but does interact with other glycophorins such as Glycophorin B. These interactions help to form the MN and Ss blood group antigens.
Glycophorin A is involved in several pathways including the erythrocyte development and lipid raft pathways. These pathways are important for cell signaling and membrane transport processes. Glycophorin A's role in erythrocyte development is connected to protein 4.1R which links the membrane to the underlying cytoskeleton influencing red blood cell shape and stability. Additionally its role in lipid raft-mediated signaling intersects with proteins involved in immune response regulation.
Glycophorin A has associations with several conditions including malaria and hereditary spherocytosis. The interaction between Plasmodium falciparum and Glycophorin A contributes to the parasite's ability to invade erythrocytes indicating its relevance in malaria research. Additionally abnormalities with Glycophorin A expression or structure can lead to hereditary spherocytosis often related to a disruption in its interaction with ankyrin another structural protein. Understanding these associations helps elucidate the pathological mechanisms and potential therapeutic targets for these diseases.
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Immunohistochemical analysis of paraffin-embedded Human angiosarcoma tissue labeling Glycophorin A with ab212432 at 0.5 μg/ml.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Glycophorin A with ab212432 at 0.5 μg/ml.
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