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AB7503

Anti-Glycophorin A antibody [JC159], prediluted

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(2 Publications)

Mouse Monoclonal Glycophorin A antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD235a, GPA, MNS, GYPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [JC159], prediluted (AB7503)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [JC159], prediluted (AB7503)

Formalin fixed paraffin embedded human tonsil tissue, staining Glycophorin A with ab7503 in immunohistochemical analysis

  • 519 FITC

    FITC Anti-Glycophorin A antibody [JC159]

  • 578 PE

    PE Anti-Glycophorin A antibody [JC159]

  • 667 PE/Cy5®

    PE/Cy5® Anti-Glycophorin A antibody [JC159]

  • 785 APC/Cy7®

    APC/Cy7® Anti-Glycophorin A antibody [JC159]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

JC159

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Specificity

This antibody is specific to erythroid cells at various stages of differentiation from the erythroblast to the mature red cell.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycophorin A also known as CD235a is a major glycoprotein of red blood cell membranes with a mass around 31 kDa. It is importantly expressed on the surface of erythrocytes. Glycophorin A plays an important role in maintaining cell membrane integrity and is associated with various antigenic sites which are important for compatibility testing. Researchers often detect Glycophorin A using antibodies such as Glycophorin A APC or Glycophorin A FITC in flow cytometry studies due to its distinct expression on red blood cells.
Biological function summary

Glycophorin A interacts with other membrane proteins to form part of the membrane's skeletal protein network. This network stabilizes the erythrocyte structure and contributes to its flexibility essential for passing through narrow capillaries. Glycophorin A does not form part of any large multiprotein complex but does interact with other glycophorins such as Glycophorin B. These interactions help to form the MN and Ss blood group antigens.

Pathways

Glycophorin A is involved in several pathways including the erythrocyte development and lipid raft pathways. These pathways are important for cell signaling and membrane transport processes. Glycophorin A's role in erythrocyte development is connected to protein 4.1R which links the membrane to the underlying cytoskeleton influencing red blood cell shape and stability. Additionally its role in lipid raft-mediated signaling intersects with proteins involved in immune response regulation.

Glycophorin A has associations with several conditions including malaria and hereditary spherocytosis. The interaction between Plasmodium falciparum and Glycophorin A contributes to the parasite's ability to invade erythrocytes indicating its relevance in malaria research. Additionally abnormalities with Glycophorin A expression or structure can lead to hereditary spherocytosis often related to a disruption in its interaction with ankyrin another structural protein. Understanding these associations helps elucidate the pathological mechanisms and potential therapeutic targets for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane (PubMed : 35835865). Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane.. (Microbial infection) Appears to be a receptor for Hepatitis A virus (HAV).. (Microbial infection) Receptor for P.falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans.
See full target information GYPA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3905 PubMed40280928

2025

T-betCD8 T cells govern anti-PD-1 responses in microsatellite-stable gastric cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Shiying Tang,Xiaofang Che,Jinyan Wang,Ce Li,Xin He,Kezuo Hou,Xiaojie Zhang,Jia Guo,Bowen Yang,Danni Li,Lili Cao,Xiujuan Qu,Zhenning Wang,Yunpeng Liu

Nature communications 14:6104 PubMed37775549

2023

Photostimulation of brain lymphatics in male newborn and adult rodents for therapy of intraventricular hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyu Li,Shaojun Liu,Tingting Yu,Zhang Liu,Silin Sun,Denis Bragin,Alexander Shirokov,Nikita Navolokin,Olga Bragina,Zhengwu Hu,Jürgen Kurths,Ivan Fedosov,Inna Blokhina,Alexander Dubrovski,Alexander Khorovodov,Andrey Terskov,Maria Tzoy,Oxana Semyachkina-Glushkovskaya,Dan Zhu
View all publications
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