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AB15009

Anti-Glycophorin A + B antibody [HIR2]

5

(2 Reviews)

|

(6 Publications)

Mouse Monoclonal Glycophorin A antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human GYPA.

View Alternative Names

CD235a, GPA, MNS, GYPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD235b, GPB, GYPB, Glycophorin-B, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A + B antibody [HIR2] (AB15009)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A + B antibody [HIR2] (AB15009)

ab15009 staining Human normal lung tissue. Staining is localised to cellular membranes. Left panel : with primary antibody at 1 μg/ml. Right panel : isotype control. Sections were stained using an automated system DAKO Autostainer Plus, at RT. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins followed by blocking with Dako Protein block for 10 mins (containing casein 0.25% in PBS) then incubated with primary antibody for 20 mins and detected with Dako Envision Flex amplification kit for 30 mins. Colorimetric detection was completed with DAB for 5 mins. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Flow Cytometry - Anti-Glycophorin A + B antibody [HIR2] (AB15009)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Glycophorin A + B antibody [HIR2] (AB15009)

Overlay histogram showing K562 cells stained with ab15009 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab15009, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Flow Cytometry - Anti-Glycophorin A + B antibody [HIR2] (AB15009)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Glycophorin A + B antibody [HIR2] (AB15009)

Flow cytometry analysis of human peripheral blood cells labelling Glycophorin A + B with ab15009. A APC-conjugated goat anti-mouse IgG was used as the secondary antibody.

  • Biotin

    Biotin Anti-Glycophorin A + B antibody [HIR2]

  • 519 FITC

    FITC Anti-Glycophorin A + B antibody [HIR2]

  • 578 PE

    PE Anti-Glycophorin A + B antibody [HIR2]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD235a antibody [HIR2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HIR2

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Human GYPA. The exact immunogen used to generate this antibody is proprietary information.

P02724

Specificity

The antibody recognizes N-terminal, homologous portion of glycophorins A (GPA) and B (GPB), (strongly to GPA, and weakly to GPB). The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycophorin A and Glycophorin B are well-known sialoglycoproteins located on the surface of human erythrocytes. They are often referenced by alternate names like GYPHE for Glycophorin E and Glycophorin A+B for the combination of both proteins. Glycophorin A which can be labeled with FITC for detection has a molecular mass of approximately 31.3 kDa while Glycophorin B has a similar but slightly smaller mass. These proteins are essential components of the erythrocyte membrane contributing significantly to the negative charge and structural stability of red blood cells.
Biological function summary

Glycophorins A and B interact with other proteins in the erythrocyte membrane influencing the cell's architecture and the stability of the cytoskeleton. These glycophorins associate with CD235a known for its role in red blood cell recognition and interactions. Glycophorin A participates in the formation of a protein complex with other membrane proteins contributing to the antigenic properties of red blood cells which is important in blood transfusion biology.

Pathways

Glycophorin A and B participate in several key biological processes such as membrane organization and signal transduction pathways. They are involved in the MNS blood group system and interact with proteins like band 3 which is a critical component of the erythrocyte's anion exchange mechanism. This interaction helps facilitate ion transport and gas exchange in red blood cells.

Glycophorins A and B have associations with malaria and autoimmune hemolytic anemia. The malaria parasite Plasmodium falciparum interacts with these glycophorins to invade red blood cells. Changes in the glycan composition of glycophorin A contribute to the pathophysiology of certain autoimmune disorders potentially linking to immune recognition proteins. Managing these interactions and modifications can be important in designing therapeutic interventions for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane (PubMed : 35835865). Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane.. (Microbial infection) Appears to be a receptor for Hepatitis A virus (HAV).. (Microbial infection) Receptor for P.falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans.
See full target information GYPA

Additional targets

GYPB
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:2661 PubMed39837851

2025

Quantifying glucose uptake at the single cell level with confocal microscopy reveals significant variability within and across individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Joel D Paprocki,Patrick J Macdonald,Yongjin Xu,Alan Cheng,Timothy C Dunn,Sergey Y Tetin

Clinical proteomics 10:1 PubMed23286773

2013

Proteomic analysis of ERK1/2-mediated human sickle red blood cell membrane protein phosphorylation.

Applications

IP, WB

Species

Human, Human

Erik J Soderblom,J Will Thompson,Evan A Schwartz,Edward Chiou,Laura G Dubois,M Arthur Moseley,Rahima Zennadi

Oncogene 32:2249-60, 2260.e1-21 PubMed22777357

2012

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

X Zhang,F D Cruz,M Terry,F Remotti,I Matushansky

Forensic science international. Genetics 2:363-71 PubMed19083849

2008

A novel fluorescence-based method in forensic science for the detection of blood in situ.

Applications

ICC/IF

Species

Human

Richard Thorogate,Joana C S Moreira,Sue Jickells,Margherita M P Miele,Barbara Daniel

Experimental hematology 24:597-604 PubMed8605964

1996

Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship with burst-forming units-erythroid.

Applications

Unspecified application

Species

Unspecified reactive species

C E Rogers,M S Bradley,B O Palsson,M R Koller

Leukemia & lymphoma 13:401-9 PubMed8069185

1994

Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers.

Applications

Unspecified application

Species

Unspecified reactive species

T Nakahata,N Okumura
View all publications
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