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AB175257

Anti-Glycophorin C/GPC antibody

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(2 Publications )

Rabbit Polyclonal Glycophorin C/GPC antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GYPC.

View Alternative Names

CD236, GLPC, GPC, GYPC, Glycophorin-C, Glycoconnectin, Glycophorin-D, Glycoprotein beta, PAS-2', Sialoglycoprotein D, GPD

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody (AB175257)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody (AB175257)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling GYPC with ab175257 at 1/200. Magnification : 200x.

Immunocytochemistry/ Immunofluorescence - Anti-Glycophorin C/GPC antibody (AB175257)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Glycophorin C/GPC antibody (AB175257)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175257. Blue DAPI for nuclear staining.

Western blot - Anti-Glycophorin C/GPC antibody (AB175257)
  • WB

Supplier Data

Western blot - Anti-Glycophorin C/GPC antibody (AB175257)

All lanes:

Western blot - Anti-Glycophorin C/GPC antibody (ab175257) at 1/500 dilution

Lane 1:

TF-1 cell line lysate

Lane 2:

K562 cell line lysate

Lane 3:

Red blood cells

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GYPC.

P04921

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycophorin C also known as GPC is a membrane glycoprotein with a molecular mass of approximately 32 kDa. GPC is recognized by other names such as Glycophorin A-like and it is a significant component of the erythrocyte membrane. Researchers frequently refer to glycophorin C by its common abbreviation GPC. GPC expression occurs specifically in red blood cells where it plays a role in maintaining the cell structure and integrity by forming part of the interface between the cell membrane and the cytoskeleton.
Biological function summary

Glycophorin C modulates red blood cell characteristics by interacting with band 4.1 protein complex. The protein participates in the regulation of cell shape and mechanical flexibility which are important for the red blood cells to flow through capillaries effectively. This process involves interactions with spectrin and actin within the cytoskeleton underpinning the cellular architecture. The protein's function as an anchor for the band 4.1 complex links it with other glycophorins such as Glycophorin A.

Pathways

GPC integrates into erythrocyte membrane-associated pathways supporting membrane stability. It is key to the cytoskeleton-membrane linkage pathway where it interacts with proteins like spectrin and actin. Aimed at preserving the biconcave shape of erythrocytes these interactions enhance membrane elasticity and durability ensuring proper red blood cell function in the circulation. Additionally its association with the protein Rh-linked ankyrin connects GPC with pathways involving erythrocyte ion transport processes.

GPC plays a notable role in elliptocytosis and malaria. Elliptocytosis arises from mutations affecting GPC and other erythrocyte proteins such as band 4.1 leading to elongated and dysfunctional erythrocytes. Individuals with altered GPC expression show increased susceptibility or resistance to malaria caused by the Plasmodium parasite’s interaction with erythrocyte membrane proteins. Glycophorin C therefore remains an important focus of study for understanding the molecular basis of these conditions and designing therapeutic interventions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.
See full target information GYPC

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:12309 PubMed37516749

2023

Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyoung Lee,Yeonjoo Kim,Paris Ataliotis,Hyung-Goo Kim,Dae-Won Kim,Dorothy C Bennett,Nigel A Brown,Lawrence C Layman,Soo-Hyun Kim

Nature communications 11:4015 PubMed32782246

2020

Analysis of erythrocyte signalling pathways during Plasmodium falciparum infection identifies targets for host-directed antimalarial intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Jack D Adderley,Simona John von Freyend,Sarah A Jackson,Megan J Bird,Amy L Burns,Burcu Anar,Tom Metcalf,Jean-Philippe Semblat,Oliver Billker,Danny W Wilson,Christian Doerig
View all publications

Product promise

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