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AB108925

Anti-Glycophorin C/GPC antibody [EPR4116]

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(1 Publication)

Rabbit Recombinant Monoclonal Glycophorin C/GPC antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD236, GLPC, GPC, GYPC, Glycophorin-C, Glycoconnectin, Glycophorin-D, Glycoprotein beta, PAS-2', Sialoglycoprotein D, GPD

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling Glycophorin C/GPC with Purified ab108925 at 1 : 1500 dilution (0.06 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Glycophorin C/GPC with Purified ab108925 at 1 : 1500 dilution (0.06 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)

Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell, Left) / K-562 (Human chronic myelogenous leukemia lymphoblast, Right) cells labeling Glycophorin C/GPC with purified ab108925 at 1/10000 dilution (0.1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Negative control : A549.

Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)
  • IP

Lab

Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)

Glycophorin C/GPC was immunoprecipitated from 0.35 mg TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 μg with 108925 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 μg

Lane 2 : ab108925 IP in TF-1 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab108925 in TF-1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Lower band could be N-Deglycosylated GPC. (PMID : 16461900)

All lanes:

Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)

Predicted band size: 14 kDa

Observed band size: 40 kDa

false

Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)
  • WB

Unknown

Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] (AB108925)

All lanes:

Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925) at 1/1000 dilution

Lane 1:

TF-1 (Human Erythroleukemia erythroblast) whole cell lysate

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lane 3:

Human red blood lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 14 kDa

false

  • Carrier free

    Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free

  • 578 PE

    PE Anti-Glycophorin C/GPC antibody [EPR4116]

  • 660 APC

    APC Anti-Glycophorin C/GPC antibody [EPR4116]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4116

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycophorin C also known as GPC is a membrane glycoprotein with a molecular mass of approximately 32 kDa. GPC is recognized by other names such as Glycophorin A-like and it is a significant component of the erythrocyte membrane. Researchers frequently refer to glycophorin C by its common abbreviation GPC. GPC expression occurs specifically in red blood cells where it plays a role in maintaining the cell structure and integrity by forming part of the interface between the cell membrane and the cytoskeleton.
Biological function summary

Glycophorin C modulates red blood cell characteristics by interacting with band 4.1 protein complex. The protein participates in the regulation of cell shape and mechanical flexibility which are important for the red blood cells to flow through capillaries effectively. This process involves interactions with spectrin and actin within the cytoskeleton underpinning the cellular architecture. The protein's function as an anchor for the band 4.1 complex links it with other glycophorins such as Glycophorin A.

Pathways

GPC integrates into erythrocyte membrane-associated pathways supporting membrane stability. It is key to the cytoskeleton-membrane linkage pathway where it interacts with proteins like spectrin and actin. Aimed at preserving the biconcave shape of erythrocytes these interactions enhance membrane elasticity and durability ensuring proper red blood cell function in the circulation. Additionally its association with the protein Rh-linked ankyrin connects GPC with pathways involving erythrocyte ion transport processes.

GPC plays a notable role in elliptocytosis and malaria. Elliptocytosis arises from mutations affecting GPC and other erythrocyte proteins such as band 4.1 leading to elongated and dysfunctional erythrocytes. Individuals with altered GPC expression show increased susceptibility or resistance to malaria caused by the Plasmodium parasite’s interaction with erythrocyte membrane proteins. Glycophorin C therefore remains an important focus of study for understanding the molecular basis of these conditions and designing therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.
See full target information GYPC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 20:2 PubMed36609216

2023

Investigation on the mechanisms of human sperm DNA damage based on the proteomics analysis by SWATH-MS.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Hui Zhu,Ye Wei,Fang Chen,Feng Li,Sheng-Min Zhang,Nai-Jun Dong,Tong-Min Xue,Kai-Feng Liu,Heng-Mi Cui,Jin-Chun Lu
View all publications
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