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Rabbit Recombinant Monoclonal Glycophorin C/GPC antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (AB247712), expandable thumbnail
  • Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (AB247712), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (AB247712), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (AB247712), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (AB247712), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.

Alternative names

CD236, GLPC, GPC, GYPC, Glycophorin-C, Glycoconnectin, Glycophorin-D, Glycoprotein beta, PAS-2', Sialoglycoprotein D, GPD

Recommended products

Rabbit Recombinant Monoclonal Glycophorin C/GPC antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4116
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247712 is the carrier-free version of Anti-Glycophorin C/GPC antibody [EPR4116] ab108925.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glycophorin C also known as GPC is a membrane glycoprotein with a molecular mass of approximately 32 kDa. GPC is recognized by other names such as Glycophorin A-like and it is a significant component of the erythrocyte membrane. Researchers frequently refer to glycophorin C by its common abbreviation GPC. GPC expression occurs specifically in red blood cells where it plays a role in maintaining the cell structure and integrity by forming part of the interface between the cell membrane and the cytoskeleton.

Biological function summary

Glycophorin C modulates red blood cell characteristics by interacting with band 4.1 protein complex. The protein participates in the regulation of cell shape and mechanical flexibility which are important for the red blood cells to flow through capillaries effectively. This process involves interactions with spectrin and actin within the cytoskeleton underpinning the cellular architecture. The protein's function as an anchor for the band 4.1 complex links it with other glycophorins such as Glycophorin A.

Pathways

GPC integrates into erythrocyte membrane-associated pathways supporting membrane stability. It is key to the cytoskeleton-membrane linkage pathway where it interacts with proteins like spectrin and actin. Aimed at preserving the biconcave shape of erythrocytes these interactions enhance membrane elasticity and durability ensuring proper red blood cell function in the circulation. Additionally its association with the protein Rh-linked ankyrin connects GPC with pathways involving erythrocyte ion transport processes.

Associated diseases and disorders

GPC plays a notable role in elliptocytosis and malaria. Elliptocytosis arises from mutations affecting GPC and other erythrocyte proteins such as band 4.1 leading to elongated and dysfunctional erythrocytes. Individuals with altered GPC expression show increased susceptibility or resistance to malaria caused by the Plasmodium parasite’s interaction with erythrocyte membrane proteins. Glycophorin C therefore remains an important focus of study for understanding the molecular basis of these conditions and designing therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712), expandable thumbnail

    Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)

    All lanes: Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] (Anti-Glycophorin C/GPC antibody [EPR4116] ab108925) at 1/1000 dilution

    Lane 1: TF-1 (Human Erythroleukemia erythroblast) whole cell lysate

    Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lane 3: Human red blood lysate

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 14 kDa

  • Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712), expandable thumbnail

    Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)

    This data was developed using Anti-Glycophorin C/GPC antibody [EPR4116] ab108925, the same antibody clone in a different buffer formulation.Glycophorin C/GPC was immunoprecipitated from 0.35 mg TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 µg with Anti-Glycophorin C/GPC antibody [EPR4116] ab108925 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 µg

    Lane 2: abab108925 IP in TF-1 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Glycophorin C/GPC antibody [EPR4116] ab108925 in TF-1 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Lower band could be N-Deglycosylated GPC. (PMID: 16461900)

    All lanes: Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] (Anti-Glycophorin C/GPC antibody [EPR4116] ab108925)

    Predicted band size: 14 kDa

    Observed band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)

    This data was developed using Anti-Glycophorin C/GPC antibody [EPR4116] ab108925, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Glycophorin C/GPC with Purified Anti-Glycophorin C/GPC antibody [EPR4116] ab108925 at 1:1500 dilution (0.06 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)

    This data was developed using Anti-Glycophorin C/GPC antibody [EPR4116] ab108925, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling Glycophorin C/GPC with Purified Anti-Glycophorin C/GPC antibody [EPR4116] ab108925 at 1:1500 dilution (0.06 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. M3

  • Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)

    This data was developed using Anti-Glycophorin C/GPC antibody [EPR4116] ab108925, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell, Left) / K-562 (Human chronic myelogenous leukemia lymphoblast, Right) cells labeling Glycophorin C/GPC with purified Anti-Glycophorin C/GPC antibody [EPR4116] ab108925 at 1/10000 dilution (0.1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    Negative control: A549.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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