AB226855

Anti-Glypican 1/ GPC1 antibody

Name: Anti-Glypican 1/ GPC1 antibody (ab226855) | Abcam
Brand: Abcam Limited
SKU: AB226855

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(1 Publication)

Rabbit Polyclonal Glypican 1/ GPC1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GPC1.

View Alternative Names

Glypican-1, GPC1

3 Images

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody (AB226855)

Paraffin-embedded human pancreatic cancer tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody (AB226855)

Paraffin-embedded human pancreatic tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Glypican 1/ GPC1 antibody (AB226855)

7.5% SDS-PAGE gel.

Lanes 1 - 4:

Western blot - Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/1000 dilution

Lanes 5 - 6:

Western blot - Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/500 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 2:

A431 cells treated with 24 µg/ml Tunicamycin for 16 hours, whole cell extract at 30 µg

Lane 3:

A431 cells treated with 4 mM Benzyl glucosinolate for 32 hours, whole cell extract at 30 µg

Lane 4:

A431 cells treated with both 24 µg/ml Tunicamycin and 4 mM Benzyl glucosinolate, whole cell extract at 30 µg

Lane 5:

MCF7 (human breast adenocarcinoma cell line) whole cell extract at 30 µg

Lane 6:

MCF7 cells treated with Glycipan 1/GPC1 shRNA, whole cell extract at 30 µg

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GPC1. The exact immunogen used to generate this antibody is proprietary information.

P35052

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glypican 1 (GPC1) also known as glypican-1 is a heparan sulfate proteoglycan anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. This protein belongs to the glypican family and has an approximate mass of 60 to 70 kDa. GPC1 is found mainly on the surface of cells in the central nervous system as well as in muscle tissues and certain epithelial cells. Its structure facilitates interaction with cell surface receptors and extracellular matrix components playing an important role in cellular communication and signaling processes.

Biological function summary

GPC1 influences the regulation of growth factors and morphogens and it has a significant role in the cell division and growth processes. It is not recognized as part of any larger protein complex but interacts with diverse ligand molecules. Researchers identify GPC1 as an important facilitator of fibroblast growth factors (FGF) and sonic hedgehog (Shh) signaling. Its interaction with molecular partners helps to regulate developmental signals important for proper cellular function and organ development.

Pathways

The contributions of GPC1 are observed in the hedgehog signaling pathway and fibroblast growth factor signaling. GPC1's interaction within the hedgehog pathway is critical for organizing cellular growth and differentiation while its involvement in the FGF pathway supports angiogenesis and wound healing. GPC1 coordinates with other glypican family members and related proteins like FGFR1 to mediate these signaling processes effectively.

Researchers have linked GPC1 with cancer progression and neurodegenerative diseases. Abnormal GPC1 expression is often noted in pancreatic cancer influencing tumor growth and metastasis through its modulation of growth factor signaling. Moreover its role in neurodegenerative conditions such as Alzheimer's disease establishes connections with amyloid-beta peptides and tau proteins contributing to disease pathogenesis. These associations make GPC1 an intriguing target for therapeutic intervention and a focus for ongoing medical research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

See full target information GPC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 63:101152 PubMed40096771

2025

Targeting BARD1 suppresses a Myc-dependent transcriptional program and tumor growth in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sohum Patel,Eleanor Jenkins,Rutuj P Kusurkar,Sherry Lee,Wei Jiang,Avinoam Nevler,Matthew McCoy,Michael J Pishvaian,Rosalie C Sears,Jonathan R Brody,Charles J Yeo,Aditi Jain

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glypican 1/ GPC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody (AB226855)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody (AB226855)

Western blot - Anti-Glypican 1/ GPC1 antibody (AB226855)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise