Anti-Glypican 1/ GPC1 antibody [EPR19285]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB199343)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membrane associated and cytoplasmic staining on MDA-MB-231 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab150077 at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB199343)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on MCF7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab150077 at 1/1000 dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB199343)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/60 dilution (red) compared with a RabbitIgG,monoclonal[EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti Rabbit IgG (Alexa Fluorr^® 488) at 1/2000 dilution was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB199343)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with the literature (PMID : 9802880).

All lanes:

Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343) at 1/1000 dilution

Lane 1:

MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Lane 5:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 55 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB199343)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 5 seconds; Lanes 2 and 3 : 10 seconds.

All lanes:

Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human colon lysate at 10 µg

Lane 3:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 61 kDa

Observed band size: 55 kDa

false