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AB174851

Anti-Glypican 3 antibody [EPR10641]

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(3 Publications)

Rabbit Recombinant Monoclonal Glypican 3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

OCI5, GPC3, Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7

3 Images
Western blot - Anti-Glypican 3 antibody [EPR10641] (AB174851)
  • WB

Lab

Western blot - Anti-Glypican 3 antibody [EPR10641] (AB174851)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : GPC3 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HepG2 whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab174851 observed at 70 kDa. Red - loading control, ab130007, observed at 125 kDa.

ab174851 was shown to recognize Glypican 3 in wild-type HAP1 cells as signal was lost at the expected MW in GPC3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPC3 knockout samples were subjected to SDS-PAGE. ab174851 and ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)

Predicted band size: 66 kDa

false

Flow Cytometry (Intracellular) - Anti-Glypican 3 antibody [EPR10641] (AB174851)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Glypican 3 antibody [EPR10641] (AB174851)

Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling Glypican 3 using ab174851 at 1/10 dilution (red) or a rabbit IgG negative (green).

Western blot - Anti-Glypican 3 antibody [EPR10641] (AB174851)
  • WB

Supplier Data

Western blot - Anti-Glypican 3 antibody [EPR10641] (AB174851)

All lanes:

Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Predicted band size: 66 kDa

false

  • Carrier free

    Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10641

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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AB267640

Human Glypican 3 ELISA Kit

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View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glypican 3 also known as GPC3 is a cell surface heparan sulfate proteoglycan with a core protein mass of approximately 70 kDa. This target is anchored to the external cell membrane through a glycosylphosphatidylinositol (GPI) anchor. Glypican 3 is expressed mainly in tissues such as the liver and kidneys and plays a role in modulating cell growth and development. In research studies it is often studied in various species including in the mouse and cynomolgus monkey models where it's referred to as mouse glypican 3 and cynomolgus glypican 3 respectively.
Biological function summary

Glypican 3 is involved in the modulation of signaling pathways that control cell proliferation and differentiation. It does not function as an isolated element; it has a role as part of a complex network that interacts with growth factors morphogens and other proteoglycans. Glypican 3 assists in the regulation of important biological events by influencing the accessibility and activity of signaling molecules on the cell surface. Its heparan sulfate chains interact with multiple proteins modifying their function and availability.

Pathways

Glypican 3 plays a significant part in the Wnt and Hedgehog signaling pathways. In the Wnt pathway it regulates the distribution and gradient formation of Wnt proteins which are essential for proper embryonic development. In the Hedgehog pathway glypican 3 modulates the distribution and activity of Hedgehog proteins important for tissue patterning and organogenesis. Other proteins closely related to glypican 3 in these pathways include those from the frizzled family in the Wnt pathway and Patched proteins in the Hedgehog pathway.

Glypican 3 has a strong connection to hepatocellular carcinoma (HCC) and Simpson-Golabi-Behmel syndrome (SGBS). In HCC increased expression of glypican 3 is often observed which associates it with tumor growth and proliferation. It functions alongside other oncogenic factors like alpha-fetoprotein (AFP) which is another marker for liver cancer. In SGBS mutations in the GPC3 gene result in defects in cellular signaling pathways leading to developmental abnormalities. This syndrome highlights the impact of glypican 3 on growth regulation and its interactions with insulin-like growth factors (IGFs).
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface proteoglycan (PubMed : 14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed : 16227623, PubMed : 24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed : 17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity).
See full target information GPC3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Liver international : official journal of the International Association for the Study of the Liver 42:696-709 PubMed34854209

2021

Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Fu,Yixuan Yang,Dazhi Zhang

Journal of bone oncology 30:100391 PubMed34611509

2021

Identification of GPC3 mutation and upregulation in a multidrug resistant osteosarcoma and its spheroids as therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Hua Nie,Tao Yang,Hong Li,Hai-Shan Ye,Guo-Qing Zhong,Ting-Ting Li,Chi Zhang,Wen-Han Huang,Jin Xiao,Zhi Li,Jian-Li He,Bo-Le Du,Yu Zhang,Jia Liu

Frontiers in oncology 11:699475 PubMed34458143

2021

Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fengpeng Wu,Bingyue Wu,Xiaoxiao Zhang,Congrong Yang,Chaoxi Zhou,Shuguang Ren,Jun Wang,Yafan Yang,Guiying Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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