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AB323324

Anti-Glypican 3 antibody [EPR24420-M2] - BSA and Azide free (Detector)

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Rabbit Recombinant Multiclonal Glypican 3 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Glypican-3, Gpc3

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Sandwich ELISA - Anti-Glypican 3 antibody [EPR24420-M2] - BSA and Azide free (Detector) (AB323324)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Glypican 3 antibody [EPR24420-M2] - BSA and Azide free (Detector) (AB323324)

Sandwich ELISA of ab323363 with the capture antibody ab323323 dilution at 2 ug/mL and detector antibody ab323324 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-Glypican 3 antibody [EPR24420-M2] - BSA and Azide free (Detector) (AB323324)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Glypican 3 antibody [EPR24420-M2] - BSA and Azide free (Detector) (AB323324)

Interpolated concentration of native Glypican 3 was measured at different sample concentrations. Undiluted samples are as follows : mouse lung extract (250 µg/mL), mouse placenta extract (62.5 µg/mL) and Hepa1-6 cell extract (125 µg/mL). Sample dilutions are made in antibody diluent CP (ab282211). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

EPR24420-M2

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab323324 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Glypican 3. The recommended pair for sandwich ELISA is:

Capture: ab323323, Mouse Glypican 3 Capture Antibody (unconjugated)

Detector: ab323324, Mouse Glypican 3 Detector Antibody (unconjugated)

The reference range value is 0.31-20 ng/mL.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface proteoglycan (By similarity). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (PubMed : 18477453, PubMed : 23665349). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (PubMed : 18477453). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (By similarity). Positively regulates the non-canonical Wnt signaling pathway (PubMed : 15537637). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (PubMed : 23665349). Inhibits the dipeptidyl peptidase activity of DPP4 (By similarity). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (PubMed : 10964473). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (PubMed : 11180950). Required for coronary vascular development (PubMed : 19733558). Plays a role in regulating cell movements during gastrulation (By similarity).
See full target information Gpc3
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Product promise

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