Rabbit Recombinant Multiclonal Glypican 3 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Multiclonal
sELISA | |
---|---|
Mouse | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Cell surface proteoglycan (By similarity). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (PubMed:18477453, PubMed:23665349). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (PubMed:18477453). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (By similarity). Positively regulates the non-canonical Wnt signaling pathway (PubMed:15537637). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (PubMed:23665349). Inhibits the dipeptidyl peptidase activity of DPP4 (By similarity). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (PubMed:10964473). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (PubMed:11180950). Required for coronary vascular development (PubMed:19733558). Plays a role in regulating cell movements during gastrulation (By similarity).
Glypican-3, Gpc3
Rabbit Recombinant Multiclonal Glypican 3 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Multiclonal
Yes
EPR24420-M2
Affinity purification Protein A
Blue Ice
+4°C
+4°C
ab323324 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Glypican 3. The recommended pair for sandwich ELISA is:
Capture: Anti-Glypican 3 antibody [EPR24420-M1] - BSA and Azide free (Capture) ab323323, Mouse Glypican 3 Capture Antibody (unconjugated)
Detector: ab323324, Mouse Glypican 3 Detector Antibody (unconjugated)
The reference range value is 0.31-20 ng/mL.
This product is a recombinant multiclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Interpolated concentration of native Glypican 3 was measured at different sample concentrations. Undiluted samples are as follows: mouse lung extract (250 µg/mL), mouse placenta extract (62.5 µg/mL) and Hepa1-6 cell extract (125 µg/mL). Sample dilutions are made in antibody diluent CP (Antibody Diluent CP ab282211). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
Sandwich ELISA of Mouse Glypican 3 Antibody Pair - BSA and Azide free ab323363 with the capture antibody Anti-Glypican 3 antibody [EPR24420-M1] - BSA and Azide free (Capture) ab323323 dilution at 2 ug/mL and detector antibody ab323324 dilution at 0.5 ug/mL.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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