Rabbit Recombinant Monoclonal Glypican 3 antibody. Carrier free. Suitable for WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IP | Flow Cyt | WB | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Cell surface proteoglycan (PubMed:14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed:16227623, PubMed:24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed:17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity).
OCI5, GPC3, Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7
Rabbit Recombinant Monoclonal Glypican 3 antibody. Carrier free. Suitable for WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR5547
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab221499 is the carrier-free version of Anti-Glypican 3 antibody [EPR5547] ab124829.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Glypican 3 also known as GPC3 is a cell surface heparan sulfate proteoglycan with a core protein mass of approximately 70 kDa. This target is anchored to the external cell membrane through a glycosylphosphatidylinositol (GPI) anchor. Glypican 3 is expressed mainly in tissues such as the liver and kidneys and plays a role in modulating cell growth and development. In research studies it is often studied in various species including in the mouse and cynomolgus monkey models where it's referred to as mouse glypican 3 and cynomolgus glypican 3 respectively.
Glypican 3 is involved in the modulation of signaling pathways that control cell proliferation and differentiation. It does not function as an isolated element; it has a role as part of a complex network that interacts with growth factors morphogens and other proteoglycans. Glypican 3 assists in the regulation of important biological events by influencing the accessibility and activity of signaling molecules on the cell surface. Its heparan sulfate chains interact with multiple proteins modifying their function and availability.
Glypican 3 plays a significant part in the Wnt and Hedgehog signaling pathways. In the Wnt pathway it regulates the distribution and gradient formation of Wnt proteins which are essential for proper embryonic development. In the Hedgehog pathway glypican 3 modulates the distribution and activity of Hedgehog proteins important for tissue patterning and organogenesis. Other proteins closely related to glypican 3 in these pathways include those from the frizzled family in the Wnt pathway and Patched proteins in the Hedgehog pathway.
Glypican 3 has a strong connection to hepatocellular carcinoma (HCC) and Simpson-Golabi-Behmel syndrome (SGBS). In HCC increased expression of glypican 3 is often observed which associates it with tumor growth and proliferation. It functions alongside other oncogenic factors like alpha-fetoprotein (AFP) which is another marker for liver cancer. In SGBS mutations in the GPC3 gene result in defects in cellular signaling pathways leading to developmental abnormalities. This syndrome highlights the impact of glypican 3 on growth regulation and its interactions with insulin-like growth factors (IGFs).
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Glypican 3 antibody [EPR5547] ab124829, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Glypican 3 antibody [EPR5547] (Anti-Glypican 3 antibody [EPR5547] ab124829) at 1/10000 dilution
Lane 1: SH-SY5Y cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 66 kDa
This data was developed using Anti-Glypican 3 antibody [EPR5547] ab124829, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 15 seconds.
All lanes: Western blot - Anti-Glypican 3 antibody [EPR5547] (Anti-Glypican 3 antibody [EPR5547] ab124829) at 1/50000 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysates at 15 µg
Lane 2: A549 (Human lung carcinoma cell line) whole cell lysates at 15 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
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