Rabbit Polyclonal Glypican 4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GPC4 aa 200-250 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Journal of proteome research 19:2539-25522019Comparative Glycoproteomic Profiling of Human Body Fluid between Healthy Controls and Patients with Papillary Thyroid Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Zhang,Wanjun Zhao,Yang Zhao,Yonghong Mao,Tao Su,Yi Zhong,Shisheng Wang,Rui Zhai,Jingqiu Cheng,Xiang Fang,Jingqiang Zhu,Hao YangPubMed 31800250
- Brain research 1487:131-92012The connexin43-dependent transcriptome during brain development: importance of genetic background.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Iacobas et. al.PubMed 22771707
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Whole serum
- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human GPC4 aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O75487
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity).
Alternative names
Glypican-4, K-glypican, UNQ474/PRO937, GPC4
Recommended products
Rabbit Polyclonal Glypican 4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GPC4 aa 200-250 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Whole serum
- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human GPC4 aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O75487
- Purity
Whole antiserum
- Specificity
Specific for a core protein epitope (aa220-233) of human Glypican 4.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glypican 4 also known as GPC4 is a heparan sulfate proteoglycan a member of the glypican family. This protein is anchored to the cell membrane and plays a role in cellular processes by attaching to the cell surface via a glycosylphosphatidylinositol (GPI) anchor. Its mass is approximately 60-70 kDa. Glypican 4 is expressed in various tissues including the brain pancreas kidney and skeletal muscle. In these tissues Glypican 4 is involved in cell communication and modulation of signaling activities at the cell surface.
- Biological function summary
Glypican 4 influences cell growth differentiation and morphogenesis. It interacts with extracellular ligands and growth factors playing a role in developmental processes. Glypican 4 is often part of a complex with other signaling molecules including Wnt and fibroblast growth factors. These interactions help regulate processes essential for organ development and tissue patterning. Glypican 4 through these molecular associations influences signaling pathways that affect both cellular architecture and function.
- Pathways
Glypican 4 plays a role in Wnt signaling and Hedgehog pathways. These pathways are critical for embryonic development and cell fate determination. Within the Wnt signaling pathway Glypican 4 interacts with the Wnt protein modulating its distribution and activity on the cell surface. In the Hedgehog pathway Glypican 4 helps modulate hedgehog proteins like Sonic Hedgehog facilitating their movement and interaction with cell surface receptors.
- Associated diseases and disorders
Glypican 4 is connected to some cancers and metabolic disorders like diabetes. Overexpression or mutations in Glypican 4 can contribute to oncogenesis as it may lead to aberrant activation of signaling pathways making it relevant in cancers like pancreatic and brain tumors. Additionally Glypican 4's role in metabolic pathways links it to insulin-related pathways emphasizing its connection with metabolic disorders such as Type 2 diabetes. Glypican 4 interacts with proteins such as insulin receptors which further highlights its relevance in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (ab52509)
IHC image of ab52509 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52509, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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