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AB156022

Anti-Glyt2 antibody [EPR10748]

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(1 Publication)

Rabbit Recombinant Monoclonal Glyt2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GLYT2, NET1, SLC6A5, Sodium- and chloride-dependent glycine transporter 2, GlyT-2, GlyT2, Solute carrier family 6 member 5

1 Images
Western blot - Anti-Glyt2 antibody [EPR10748] (AB156022)
  • WB

Unknown

Western blot - Anti-Glyt2 antibody [EPR10748] (AB156022)

All lanes:

Western blot - Anti-Glyt2 antibody [EPR10748] (ab156022) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa,87 kDa

Observed band size: 24 kDa

false

  • Carrier free

    Anti-Glyt2 antibody [EPR10748] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10748

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glycine transporter 2 often referred to as GlyT2 plays an important role mechanically. GlyT2 is responsible for the reuptake of glycine a neurotransmitter from the synaptic cleft back into presynaptic neurons. It is a membrane-bound protein with an approximate molecular mass of 85 kDa. GlyT2 is mostly expressed in the spinal cord and brainstem where it facilitates glycine neurotransmission essential for inhibitory signaling in the central nervous system.
Biological function summary

GlyT2 functions to maintain glycine homeostasis which is important for regulating inhibitory synaptic transmission. GlyT2 operates as part of a larger protein complex that efficiently recycles glycine into neurons which is essential for synaptic vesicle refilling. This recycling mechanism ensures a sufficient supply of glycine in synaptic vesicles contributing to the modulation of neuronal excitability and prevention of excessive excitatory neurotransmission.

Pathways

GlyT2 actively participates in the glycinergic synapse pathway which controls inhibitory neurotransmission. It complements the function of glycine receptors by ensuring sustained glycine availability. Related proteins involved in this pathway include GlyT1 another transporter which works alongside GlyT2 in glycine regulation. Additionally the function of GlyT2 is closely tied to the neurotransmitter release cycle pathway which manages synaptic neurotransmitter levels and synaptic signaling fidelity.

GlyT2 is linked to certain neurological conditions due to its role in regulating glycine levels. Hyperekplexia a genetic disorder characterized by exaggerated startle responses has been associated with mutations in the GlyT2 gene. Additionally GlyT2 might play a part in pain disorders where disrupted glycinergic transmission contributes to pathophysiology. In these conditions altered GlyT2 function impacts the balance of inhibitory and excitatory signals vital for normal sensory processing with GlyT2 interacting with proteins such as inhibitory glycine receptor alpha1 in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium- and chloride-dependent glycine transporter (PubMed : 10381548, PubMed : 10606742, PubMed : 16751771, PubMed : 31370103, PubMed : 9845349). Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed : 9845349). May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses (PubMed : 9845349).. Isoform 2. Lacks sodium- and chloride-dependent glycine transporter activity.. Isoform 3. Lacks sodium- and chloride-dependent glycine transporter activity.
See full target information SLC6A5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38201261

2024

Reduction in Nuclear Size by DHRS7 in Prostate Cancer Cells and by Estradiol Propionate in DHRS7-Depleted Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Rizzotto,Sylvain Tollis,Nhan T Pham,Yijing Zheng,Maria Alba Abad,Jan Wildenhain,A Arockia Jeyaprakash,Manfred Auer,Mike Tyers,Eric C Schirmer
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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