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AB249261

Anti-Glyt2 antibody [EPR10748] - BSA and Azide free

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Rabbit Recombinant Monoclonal Glyt2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

GLYT2, NET1, SLC6A5, Sodium- and chloride-dependent glycine transporter 2, GlyT-2, GlyT2, Solute carrier family 6 member 5

1 Images
Western blot - Anti-Glyt2 antibody [EPR10748] - BSA and Azide free (AB249261)
  • WB

Unknown

Western blot - Anti-Glyt2 antibody [EPR10748] - BSA and Azide free (AB249261)

This data was developed using ab156022, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Glyt2 antibody [EPR10748] (<a href='/en-us/products/primary-antibodies/glyt2-antibody-epr10748-ab156022'>ab156022</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa,87 kDa

Observed band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10748

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249261 is the carrier-free version of ab156022.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glycine transporter 2 often referred to as GlyT2 plays an important role mechanically. GlyT2 is responsible for the reuptake of glycine a neurotransmitter from the synaptic cleft back into presynaptic neurons. It is a membrane-bound protein with an approximate molecular mass of 85 kDa. GlyT2 is mostly expressed in the spinal cord and brainstem where it facilitates glycine neurotransmission essential for inhibitory signaling in the central nervous system.
Biological function summary

GlyT2 functions to maintain glycine homeostasis which is important for regulating inhibitory synaptic transmission. GlyT2 operates as part of a larger protein complex that efficiently recycles glycine into neurons which is essential for synaptic vesicle refilling. This recycling mechanism ensures a sufficient supply of glycine in synaptic vesicles contributing to the modulation of neuronal excitability and prevention of excessive excitatory neurotransmission.

Pathways

GlyT2 actively participates in the glycinergic synapse pathway which controls inhibitory neurotransmission. It complements the function of glycine receptors by ensuring sustained glycine availability. Related proteins involved in this pathway include GlyT1 another transporter which works alongside GlyT2 in glycine regulation. Additionally the function of GlyT2 is closely tied to the neurotransmitter release cycle pathway which manages synaptic neurotransmitter levels and synaptic signaling fidelity.

GlyT2 is linked to certain neurological conditions due to its role in regulating glycine levels. Hyperekplexia a genetic disorder characterized by exaggerated startle responses has been associated with mutations in the GlyT2 gene. Additionally GlyT2 might play a part in pain disorders where disrupted glycinergic transmission contributes to pathophysiology. In these conditions altered GlyT2 function impacts the balance of inhibitory and excitatory signals vital for normal sensory processing with GlyT2 interacting with proteins such as inhibitory glycine receptor alpha1 in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium- and chloride-dependent glycine transporter (PubMed : 10381548, PubMed : 10606742, PubMed : 16751771, PubMed : 31370103, PubMed : 9845349). Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed : 9845349). May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses (PubMed : 9845349).. Isoform 2. Lacks sodium- and chloride-dependent glycine transporter activity.. Isoform 3. Lacks sodium- and chloride-dependent glycine transporter activity.
See full target information SLC6A5
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Product promise

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For full details, please see our Terms & Conditions

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