Rabbit Recombinant Monoclonal GM-CSF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-GM-CSF antibody [EPR25991-223] (AB300495), expandable thumbnail](https://content.abcam.com/products/images/gm-csf-antibody-epr25991-223-ab300495--western-blot-img400441.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GM-CSF antibody [EPR25991-223] (AB300495)")
![Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (AB300495), expandable thumbnail](https://content.abcam.com/products/images/gm-csf-antibody-epr25991-223-ab300495--immunoprecipitation-img400565.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (AB300495)")
Publications
Filter by
- Current issues in molecular biology 45:7827-78412023T-Lymphocytes Activated by Dendritic Cells Loaded by Tumor-Derived Vesicles Decrease Viability of Melanoma Cells In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvan Yurevich Filin,Yuriy Pavlovich Mayasin,Chulpan Bulatovna Kharisova,Anna Valerevna Gorodilova,Daria Sergeevna Chulpanova,Kristina Viktorovna Kitaeva,Albert Anatolyevich Rizvanov,Valeria Vladimirovna SolovyevaPubMed 37886937
- International journal of biological sciences 19:3920-39362023PAK1 overexpression promotes myxofibrosarcoma angiogenesis through STAT5B-mediated transactivation: clinical and therapeutic relevance of amplification and nuclear entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChien-Feng Li,Ti-Chun Chan,Fu-Min Fang,Shih-Chen Yu,Hsuan-Ying HuangPubMed 37564209
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Alternative names
GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF
Recommended products
Rabbit Recombinant Monoclonal GM-CSF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25991-223
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.
- Biological function summary
GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.
- Pathways
GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.
- Associated diseases and disorders
GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-GM-CSF antibody [EPR25991-223] (ab300495), expandable thumbnail](https://content.abcam.com/products/images/gm-csf-antibody-epr25991-223-ab300495--western-blot-img400441.jpg "Western blot - Anti-GM-CSF antibody [EPR25991-223] (ab300495)")
Western blot - Anti-GM-CSF antibody [EPR25991-223] (ab300495)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 14512323).All lanes: Western blot - Anti-GM-CSF antibody [EPR25991-223] (ab300495) at 1/1000 dilution
Lane 1: Untreated HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
Lane 2: HuT-78 treated with 80nM TPA and 30uM ionomycin for 5 hours, then with /ml BFA added for 4 hours whole cell lysate 20 µg
Lane 3: Untreated Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 4: Jurkat treated with 80nM TPA and 30uM ionomycin for 5 hours, then with /ml BFA added for 4 hours whole cell lysate 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 15 kDa, 18 kDa, 22 kDa
![Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (ab300495), expandable thumbnail](https://content.abcam.com/products/images/gm-csf-antibody-epr25991-223-ab300495--immunoprecipitation-img400565.jpg "Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (ab300495)")
Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (ab300495)
CSF2 was immunoprecipitated from 0.35 mg HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30?M ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours whole cell lysate 10 µg with ab300495 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300495 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30?M ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours whole cell lysate 10 µg
Lane 2: abAB300495 IP in HuT-78 treated with 80nM TPA and 30?M ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours cell lysates
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300495 in HuT-78 treated with 80nM TPA and 30?M ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours cell lysates
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 75 seconds
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 14512323).
All lanes: Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (ab300495) at 1/1000 dilution
All lanes: HuT-78 treated with 80nM TPA and 30?M ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours cell lysates at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 75s
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