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AB300495

Anti-GM-CSF antibody [EPR25991-223]

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(6 Publications)

Rabbit Recombinant Monoclonal GM-CSF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF

2 Images
Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (AB300495)
  • IP

Supplier Data

Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (AB300495)

CSF2 was immunoprecipitated from 0.35 mg HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30μM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours whole cell lysate 10 µg with ab300495 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300495 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30μM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours whole cell lysate 10 µg

Lane 2 : ab300495 IP in HuT-78 treated with 80nM TPA and 30μM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours cell lysates

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300495 in HuT-78 treated with 80nM TPA and 30μM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours cell lysates

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 75 seconds

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 14512323).

All lanes:

Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (ab300495) at 1/1000 dilution

All lanes:

HuT-78 treated with 80nM TPA and 30μM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours cell lysates at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 75s

Western blot - Anti-GM-CSF antibody [EPR25991-223] (AB300495)
  • WB

Supplier Data

Western blot - Anti-GM-CSF antibody [EPR25991-223] (AB300495)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 14512323).

All lanes:

Western blot - Anti-GM-CSF antibody [EPR25991-223] (ab300495) at 1/1000 dilution

Lane 1:

Untreated HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate

Lane 2:

HuT-78 treated with 80nM TPA and 30uM ionomycin for 5 hours, then with /ml BFA added for 4 hours whole cell lysate 20 µg

Lane 3:

Untreated Jurkat (human T cell leukemia T lymphocyte) whole cell lysate

Lane 4:

Jurkat treated with 80nM TPA and 30uM ionomycin for 5 hours, then with /ml BFA added for 4 hours whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa,18 kDa,22 kDa

false

  • Carrier free

    Anti-GM-CSF antibody [EPR25991-223] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25991-223

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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  • 0
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Primary Antibodies

AB302730

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.
Biological function summary

GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.

Pathways

GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.

GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
See full target information CSF2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Sports medicine and health science 7:143-156 PubMed39811408

2025

Exploring the role of adipokines in exercise-induced inhibition of tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Qian,Zhenglong Bu,Yang Qin,Shiyuan Qian,Lu Qin,Siqi Zhou,Qingda Wang,Longjun Xian,Lei Hu,Yimei Xiong,Yingying Zhang,Chun Wang

Cell communication and signaling : CCS 22:478 PubMed39375718

2024

Senescent cells promote breast cancer cells motility by secreting GM-CSF and bFGF that activate the JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Wang,Yan Fang,Yigong Hou,Dongmei Cheng,Emily V Dressler,Hao Wang,Juan Wang,Guanwen Wang,Yilei Li,Hong Liu,Rong Xiang,Shuang Yang,Peiqing Sun

International journal of molecular medicine 54: PubMed39027993

2024

CD150‑dependent activation of EBV‑transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Young Kim,Il Keung Seo,Dae Young Hur

International journal of molecular sciences 25: PubMed39000311

2024

Oncolytic Activity of Sindbis Virus with the Help of GM-CSF in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangwei Shi,Kangyixin Sun,Li Li,Jingwen Xian,Ping Wang,Fan Jia,Fuqiang Xu

Current issues in molecular biology 45:7827-7841 PubMed37886937

2023

T-Lymphocytes Activated by Dendritic Cells Loaded by Tumor-Derived Vesicles Decrease Viability of Melanoma Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Yurevich Filin,Yuriy Pavlovich Mayasin,Chulpan Bulatovna Kharisova,Anna Valerevna Gorodilova,Daria Sergeevna Chulpanova,Kristina Viktorovna Kitaeva,Albert Anatolyevich Rizvanov,Valeria Vladimirovna Solovyeva

International journal of biological sciences 19:3920-3936 PubMed37564209

2023

PAK1 overexpression promotes myxofibrosarcoma angiogenesis through STAT5B-mediated transactivation: clinical and therapeutic relevance of amplification and nuclear entry.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Feng Li,Ti-Chun Chan,Fu-Min Fang,Shih-Chen Yu,Hsuan-Ying Huang
View all publications
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Product promise

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