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Rabbit Recombinant Monoclonal GM-CSF antibody. Suitable for WB, Flow Cyt (Intra), IP, ICC/IF, IHC-P and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-GM-CSF antibody [EPR28743-78] (AB316862), expandable thumbnail
  • Immunoprecipitation - Anti-GM-CSF antibody [EPR28743-78] (AB316862), expandable thumbnail
  • Western blot - Anti-GM-CSF antibody [EPR28743-78] (AB316862), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-GM-CSF antibody [EPR28743-78] (AB316862), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM-CSF antibody [EPR28743-78] (AB316862), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IPICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200 - 1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Alternative names

GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF

Recommended products

Rabbit Recombinant Monoclonal GM-CSF antibody. Suitable for WB, Flow Cyt (Intra), IP, ICC/IF, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28743-78
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.

Biological function summary

GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.

Pathways

GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.

Associated diseases and disorders

GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-GM-CSF antibody [EPR28743-78] (ab316862), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GM-CSF antibody [EPR28743-78] (ab316862)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HuT-78 (human Sezary syndrome cutaneous T lymphocyte) cells labelling GM-CSF with ab316862 at 1/50 (10.16 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing increased cytoplasmic staining in HuT-78 cells treated with Phorbol-12-myristate-13-acetate (80 nM) and Ionomycin (3 uM) for 5 hours, then add Brefeldin A (300 ng/ml) for 4 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunoprecipitation - Anti-GM-CSF antibody [EPR28743-78] (ab316862), expandable thumbnail

    Immunoprecipitation - Anti-GM-CSF antibody [EPR28743-78] (ab316862)

    GM-CSF was immunoprecipitated from 0.35 mg HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h, whole cell lysate with ab316862 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316862 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h, whole cell lysate

    Lane 2: ab316862 IP in HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h, whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316862 in HuT-78 treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h whole cell lysate

    All lanes: Immunoprecipitation - Anti-GM-CSF antibody [EPR28743-78] (ab316862) at 1/30 dilution

    All lanes: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h, whole cell lysate with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Western blot - Anti-GM-CSF antibody [EPR28743-78] (ab316862), expandable thumbnail

    Western blot - Anti-GM-CSF antibody [EPR28743-78] (ab316862)

    The identity of the higher MW band at approximately 50 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GM-CSF antibody [EPR28743-78] (ab316862) at 1/1000 dilution

    Lane 1: Untreated HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HuT-78 treated with 80nM TPA and 30uM Ionomycin for 5h, /ml BFA was then added for additional 4h, whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa, 36 kDa

    Exposure time: 37s

  • Flow Cytometry (Intracellular) - Anti-GM-CSF antibody [EPR28743-78] (ab316862), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GM-CSF antibody [EPR28743-78] (ab316862)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HuT-78(human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 3uM onomycin for 5h and add 300ng/ml BFA for 4h (Red)/Untreated HuT-78 (Dotted red) cells labelling GM-CSF with ab316862 at 1/5000 dilution (0.01ug)/Red and dotted red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/5000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM-CSF antibody [EPR28743-78] (ab316862), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM-CSF antibody [EPR28743-78] (ab316862)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling GM-CSF with ab316862 at 1/200 (2.54 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human colon carcinoma (PMID: 31908491).

    The section was incubated with ab316862 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM-CSF antibody [EPR28743-78] (ab316862), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM-CSF antibody [EPR28743-78] (ab316862)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling GM-CSF with ab316862 at 1/200 (2.54 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human lung (PMID: 32719685).

    The section was incubated with ab316862 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM-CSF antibody [EPR28743-78] (ab316862), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM-CSF antibody [EPR28743-78] (ab316862)

    Immunohistochemical analysis of paraffin-embedded (A) HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with TPA (80nM/5 hours) and ionomycin (30µM/5 hours) add BFA (300ngml/4 hours). (A) HuT-78 untreated with TPA (80nM/5 hours) and ionomycin (30µM/5 hours) add BFA (300ngml/4 hours). tissue labeling GM-CSF with ab316862 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) HuT-78 treated with TPA (80nM/5 hours) and ionomycin (30µM/5 hours) add BFA (300ngml/4 hours). No staining on (B) HuT-78 untreated with TPA (80nM/5 hours) and ionomycin (30µM/5 hours) add BFA (300ngml/4 hours).

    The section was incubated with ab316862 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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