Rabbit Recombinant Monoclonal GM-CSF antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
WB | IHC-P | Flow Cyt (Intra) | IP | ICC/IF | |
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Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF
Rabbit Recombinant Monoclonal GM-CSF antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
ab316863 is the carrier-free version of Anti-GM-CSF antibody [EPR28743-78] ab316862.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.
GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.
GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.
GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-GM-CSF antibody [EPR28743-78] ab316862, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HuT-78 (human Sezary syndrome cutaneous T lymphocyte) cells labelling GM-CSF with Anti-GM-CSF antibody [EPR28743-78] ab316862 at 1/50 (10.16 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing increased cytoplasmic staining in HuT-78 cells treated with Phorbol-12-myristate-13-acetate (80 nM) and Ionomycin (3 uM) for 5 hours, then add Brefeldin A (300 ng/ml) for 4 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
This data was developed using Anti-GM-CSF antibody [EPR28743-78] ab316862, the same antibody clone in a different buffer formulation.
GM-CSF was immunoprecipitated from 0.35 mg HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h, whole cell lysate with Anti-GM-CSF antibody [EPR28743-78] ab316862 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-GM-CSF antibody [EPR28743-78] ab316862 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h, whole cell lysate
Lane 2: Anti-GM-CSF antibody [EPR28743-78] ab316862 IP in HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GM-CSF antibody [EPR28743-78] ab316862 in HuT-78 treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h whole cell lysate
All lanes: Immunoprecipitation - Anti-GM-CSF antibody [EPR28743-78] (Anti-GM-CSF antibody [EPR28743-78] ab316862) at 1/30 dilution
All lanes: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30uM Ionomycin for 5h, 300ng/ml BFA was then added for additional 4h, whole cell lysate with NFDM/TBST
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
This data was developed using Anti-GM-CSF antibody [EPR28743-78] ab316862, the same antibody clone in a different buffer formulation.
The identity of the higher MW band at approximately 50 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-GM-CSF antibody [EPR28743-78] (Anti-GM-CSF antibody [EPR28743-78] ab316862) at 1/1000 dilution
Lane 1: Untreated HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate at 20 µg with NFDM/TBST
Lane 2: HuT-78 treated with 80nM TPA and 30uM Ionomycin for 5h, /ml BFA was then added for additional 4h, whole cell lysate at 20 µg with NFDM/TBST
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 20 kDa, 36 kDa
Exposure time: 37s
This data was developed using Anti-GM-CSF antibody [EPR28743-78] ab316862, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HuT-78(human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 3uM onomycin for 5h and add 300ng/ml BFA for 4h (Red)/Untreated HuT-78 (Dotted red) cells labelling GM-CSF with Anti-GM-CSF antibody [EPR28743-78] ab316862 at 1/5000 dilution (0.01ug)/Red and dotted red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/5000 dilution was used as the secondary antibody.
This data was developed using Anti-GM-CSF antibody [EPR28743-78] ab316862, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling GM-CSF with Anti-GM-CSF antibody [EPR28743-78] ab316862 at 1/200 (2.54 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human colon carcinoma (PMID: 31908491).
The section was incubated with Anti-GM-CSF antibody [EPR28743-78] ab316862 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-GM-CSF antibody [EPR28743-78] ab316862, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling GM-CSF with Anti-GM-CSF antibody [EPR28743-78] ab316862 at 1/200 (2.54 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human lung (PMID: 32719685).
The section was incubated with Anti-GM-CSF antibody [EPR28743-78] ab316862 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-GM-CSF antibody [EPR28743-78] ab316862, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with TPA (80nM/5 hours) and ionomycin (30µM/5 hours) add BFA (300ngml/4 hours). (A) HuT-78 untreated with TPA (80nM/5 hours) and ionomycin (30µM/5 hours) add BFA (300ngml/4 hours). tissue labeling GM-CSF with Anti-GM-CSF antibody [EPR28743-78] ab316862 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HuT-78 treated with TPA (80nM/5 hours) and ionomycin (30µM/5 hours) add BFA (300ngml/4 hours). No staining on (B) HuT-78 untreated with TPA (80nM/5 hours) and ionomycin (30µM/5 hours) add BFA (300ngml/4 hours).
The section was incubated with Anti-GM-CSF antibody [EPR28743-78] ab316862 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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