Rabbit Polyclonal GM130 antibody. cis-Golgi marker. Suitable for WB, ICC/IF and reacts with Drosophila melanogaster, Human samples. Cited in 86 publications.
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- Bioactive materials 32:488-5012023Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Zhuo Wan,Di Qu,Wenqi Sun,Liang Zhang,Yuan Liang,Lei Pan,Hua Jiang,Zichen Ye,Mengying Wei,Lijun Yuan,Guodong Yang,Faguang JinPubMed 37965241
- PLoS genetics 19:e10109792023A Rab6 to Rab11 transition is required for dense-core granule and exosome biogenesis in Drosophila secondary cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam Wells,Cláudia C Mendes,Felix Castellanos,Phoebe Mountain,Tia Wright,S Mark Wainwright,M Irina Stefana,Adrian L Harris,Deborah C I Goberdhan,Clive WilsonPubMed 37844085
- Aging and disease 14:952-9652023Exercise Improves Metabolism and Alleviates Atherosclerosis via Muscle-Derived Extracellular Vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixiao Wang,Yunnan Liu,Siyan Zhang,Na Li,Changyang Xing,Chen Wang,Jia Wang,Mengying Wei,Guodong Yang,Lijun YuanPubMed 37191422
- Development (Cambridge, England) 150:2023The Drosophila AWP1 ortholog Doctor No regulates JAK/STAT signaling for left-right asymmetry in the gut by promoting receptor endocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Ting Lai,Takeshi Sasamura,Junpei Kuroda,Reo Maeda,Mitsutoshi Nakamura,Ryo Hatori,Tomoki Ishibashi,Kiichiro Taniguchi,Masashi Ooike,Tomohiro Taguchi,Naotaka Nakazawa,Shunya Hozumi,Takashi Okumura,Toshiro Aigaki,Mikiko Inaki,Kenji MatsunoPubMed 36861793
- Journal of cell science 135:2022Functions of neuronal Synaptobrevin in the post-Golgi transport of Rhodopsin in Drosophila photoreceptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHitomi Yamashita,Yuka Ochi,Yumi Yamada,Shogo Sasaki,Tatsuya Tago,Takunori Satoh,Akiko K SatohPubMed 36444566
- Journal of extracellular vesicles 11:e122632022Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren Linnemannstöns,Pradhipa Karuna M,Leonie Witte,Dolma Choezom,Mona Honemann-Capito,Alex Simon Lagurin,Chantal Vanessa Schmidt,Shreya Shrikhande,Lara-Kristin Steinmetz,Möbius Wiebke,Christof Lenz,Julia Christina GrossPubMed 36103151
- Current biology : CB 32:4549-4564.e62022In vivo characterization of Drosophila golgins reveals redundancy and plasticity of vesicle capture at the Golgi apparatus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung Yun Park,Nadine Muschalik,Jessica Chadwick,Sean MunroPubMed 36103876
- Journal of nanobiotechnology 20:3852022ROS triggered local delivery of stealth exosomes to tumors for enhanced chemo/photodynamic therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuo Wan,Xueqi Gan,Ruiyan Mei,Jianbin Du,Wen Fan,Mengying Wei,Guodong Yang,Weiwei Qin,Zhuoli Zhu,Li LiuPubMed 35999549
- Traffic (Copenhagen, Denmark) 23:374-3902022Myosin V facilitates polarised E-cadherin secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDajana Tanasic,Nicola Berns,Veit RiechmannPubMed 35575181
- Cell reports 39:1109032022GMAP is an Atg8a-interacting protein that regulates Golgi turnover in Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshrafur Rahman,Peter Lőrincz,Raksha Gohel,Anikó Nagy,Gábor Csordás,Yan Zhang,Gábor Juhász,Ioannis P NezisPubMed 35649355
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Drosophila melanogaster | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info 0.1 µg/mL | Notes - |
Species Human | Dilution info 0.1 µg/mL | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Drosophila melanogaster | Dilution info - | Notes - |
Associated Products
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Target data
Function
Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed:16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).
Alternative names
Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, Golgin-95, GM130, GOLGA2
Recommended products
Rabbit Polyclonal GM130 antibody. cis-Golgi marker. Suitable for WB, ICC/IF and reacts with Drosophila melanogaster, Human samples. Cited in 86 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GM130 also known as Golgi matrix protein 130 kDa or GM-130 plays an important role in the organization of the Golgi apparatus. The GM130 protein is vital for maintaining the structure of the Golgi serving as a Golgi marker for researchers. Its molecular weight is approximately 130 kDa. You will commonly find GM130 expressed in cells localizing to the cis-Golgi network. As a Golgi marker it serves as an indication of the integrity and positioning of the Golgi apparatus in the cell.
- Biological function summary
GM130 serves as a structural component within the Golgi matrix. It interacts with other proteins such as GRASP65 to form a complex which is essential for Golgi stacking and maintenance of Golgi architecture. This interaction helps regulate transport processes inside cells. GM130 proteins help formation of mini-stacks and transitioning of vesicles. In cells GM130 plays a significant role to maintain efficient protein trafficking through the Golgi network.
- Pathways
GM130 interacts with proteins like Rab1 during the vesicular transport pathway. It plays a part in the transport of proteins from the endoplasmic reticulum to the Golgi apparatus. Another related pathway involves SNARE proteins whereby GM130 contributes to membrane docking and fusion events. Through these interactions GM130 modulates the fidelity and specificity of molecular cargo transport within the cell.
- Associated diseases and disorders
GM130 has a connection to neurodegenerative diseases such as frontotemporal dementia. Disruption in GM130 proteins affects Golgi structure and function potentially leading to protein trafficking defects associated with the disorder. GM130 also relates to Alzheimer's disease through its interaction with tau protein. Alterations in GM130's function can influence tau pathology contributing to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Image from Zhang L et al, J Biol Chem. 2010 Jun 18;285(25):19491-501. Epub 2010 Apr 6, Fig 1. DOI 10.1074/jbc.M109.098145 June 18, 2010 The Journal of Biological Chemistry, 285, 19491-19501.Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)
ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.
Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1.
This image is courtesy of Sean Munro, MRC Laboratory of Molecular BiologyImmunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)
The image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.
This image is courtesy of Sean Munro, MRC Laboratory of Molecular BiologyWestern blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)
All lanes: Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/mL
Lane 1: Drosophila melanogaster S2 cell extract
Lane 2: Drosophila melanogaster S2 cell extract + specific siRNA
Predicted band size: 113 kDa
Observed band size: 95 kDa
Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)
All lanes: Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/mL
Lane 1: Schneider L2 whole cell lysate (ab14893) at 10 µg
Lane 2: Schneider L2 whole cell lysate (ab14893) at 10 µg with D. melanogaster GM130 peptide (ab32335)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Predicted band size: 113 kDa
Observed band size: 105 kDa, 70 kDa
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