AB30637

Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker

Name: Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) | Abcam
Brand: Abcam Limited
SKU: AB30637
Price: 400 USD
Availability: InStock
Rating: 4 (3 reviews)

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(3 Reviews)

(94 Publications)

Rabbit Polyclonal GM130 antibody. cis-Golgi marker. Suitable for WB, ICC/IF and reacts with Drosophila melanogaster, Human samples. Cited in 94 publications.

View Alternative Names

Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, Golgin-95, GM130, GOLGA2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

The image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.

This image is courtesy of Sean Munro, MRC Laboratory of Molecular Biology

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.

Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1.

Image from Zhang L et al, J Biol Chem. 2010 Jun 18;285(25):19491-501. Epub 2010 Apr 6, Fig 1. DOI 10.1074/jbc.M109.098145 June 18, 2010 The Journal of Biological Chemistry, 285, 19491-19501.

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Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

All lanes:

Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/mL

Lane 1:

Drosophila melanogaster S2 cell extract

Lane 2:

Drosophila melanogaster S2 cell extract + specific siRNA

Predicted band size: 113 kDa

Observed band size: 95 kDa

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This image is courtesy of Sean Munro, MRC Laboratory of Molecular Biology

Unknown

Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

All lanes:

Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/mL

Lane 1:

Schneider L2 whole cell lysate (<a href='/en-us/products/unavailable/schneider-l2-whole-cell-lysate-ab14893'>ab14893</a>) at 10 µg

Lane 2:

Schneider L2 whole cell lysate (<a href='/en-us/products/unavailable/schneider-l2-whole-cell-lysate-ab14893'>ab14893</a>) at 10 µg with D. melanogaster GM130 peptide (<a href='/en-us/products/unavailable/d-melanogaster-gm130-peptide-ab32335'>ab32335</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 113 kDa

Observed band size: 105 kDa,70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Drosophila melanogaster

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GM130 also known as Golgi matrix protein 130 kDa or GM-130 plays an important role in the organization of the Golgi apparatus. The GM130 protein is vital for maintaining the structure of the Golgi serving as a Golgi marker for researchers. Its molecular weight is approximately 130 kDa. You will commonly find GM130 expressed in cells localizing to the cis-Golgi network. As a Golgi marker it serves as an indication of the integrity and positioning of the Golgi apparatus in the cell.

Biological function summary

GM130 serves as a structural component within the Golgi matrix. It interacts with other proteins such as GRASP65 to form a complex which is essential for Golgi stacking and maintenance of Golgi architecture. This interaction helps regulate transport processes inside cells. GM130 proteins help formation of mini-stacks and transitioning of vesicles. In cells GM130 plays a significant role to maintain efficient protein trafficking through the Golgi network.

Pathways

GM130 interacts with proteins like Rab1 during the vesicular transport pathway. It plays a part in the transport of proteins from the endoplasmic reticulum to the Golgi apparatus. Another related pathway involves SNARE proteins whereby GM130 contributes to membrane docking and fusion events. Through these interactions GM130 modulates the fidelity and specificity of molecular cargo transport within the cell.

GM130 has a connection to neurodegenerative diseases such as frontotemporal dementia. Disruption in GM130 proteins affects Golgi structure and function potentially leading to protein trafficking defects associated with the disorder. GM130 also relates to Alzheimer's disease through its interaction with tau protein. Alterations in GM130's function can influence tau pathology contributing to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed : 16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly : phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed : 26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle : upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed : 19242490, PubMed : 26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed : 18045989, PubMed : 19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed : 16489344, PubMed : 17314401).

See full target information Golgin subfamily A member 2

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Publications (94)

Recent publications for all applications. Explore the full list and refine your search

Open biology 15:240267 PubMed39999877

2025

PACS deficiency disrupts Golgi architecture and causes cytokinesis failures and seizure-like phenotype in .

Applications

Unspecified application

Species

Unspecified reactive species

Anna Frappaolo,Gianluca Zaccagnini,Maria Giovanna Riparbelli,Gianni Colotti,Giuliano Callaini,Maria Grazia Giansanti

Frontiers in cell and developmental biology 12:1442198 PubMed39296936

2024

Golgi clustering by the deficiency of COPI-SNARE in photoreceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuya Tago,Yumi Yamada,Yumi Goto,Kiminori Toyooka,Yuka Ochi,Takunori Satoh,Akiko K Satoh

EMBO reports 25:3574-3600 PubMed39009833

2024

Complex interplay between RAS GTPases and RASSF effectors regulates subcellular localization of YAP.

Applications

Unspecified application

Species

Unspecified reactive species

Swati Singh,Gabriela Bernal Astrain,Ana Maria Hincapie,Marilyn Goudreault,Matthew J Smith

Bioactive materials 32:488-501 PubMed37965241

2023

Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Zhuo Wan,Di Qu,Wenqi Sun,Liang Zhang,Yuan Liang,Lei Pan,Hua Jiang,Zichen Ye,Mengying Wei,Lijun Yuan,Guodong Yang,Faguang Jin

PLoS genetics 19:e1010979 PubMed37844085

2023

A Rab6 to Rab11 transition is required for dense-core granule and exosome biogenesis in Drosophila secondary cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Wells,Cláudia C Mendes,Felix Castellanos,Phoebe Mountain,Tia Wright,S Mark Wainwright,M Irina Stefana,Adrian L Harris,Deborah C I Goberdhan,Clive Wilson

eLife 12: PubMed37432316

2023

Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset.

Applications

Unspecified application

Species

Unspecified reactive species

Arian Mansur,Remi Joseph,Euri S Kim,Pierre M Jean-Beltran,Namrata D Udeshi,Cadence Pearce,Hanjie Jiang,Reina Iwase,Miroslav P Milev,Hashem A Almousa,Elyshia McNamara,Jeffrey Widrick,Claudio Perez,Gianina Ravenscroft,Michael Sacher,Philip A Cole,Steven A Carr,Vandana A Gupta

Aging and disease 14:952-965 PubMed37191422

2023

Exercise Improves Metabolism and Alleviates Atherosclerosis via Muscle-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Yixiao Wang,Yunnan Liu,Siyan Zhang,Na Li,Changyang Xing,Chen Wang,Jia Wang,Mengying Wei,Guodong Yang,Lijun Yuan

Development (Cambridge, England) 150: PubMed36861793

2023

The Drosophila AWP1 ortholog Doctor No regulates JAK/STAT signaling for left-right asymmetry in the gut by promoting receptor endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ting Lai,Takeshi Sasamura,Junpei Kuroda,Reo Maeda,Mitsutoshi Nakamura,Ryo Hatori,Tomoki Ishibashi,Kiichiro Taniguchi,Masashi Ooike,Tomohiro Taguchi,Naotaka Nakazawa,Shunya Hozumi,Takashi Okumura,Toshiro Aigaki,Mikiko Inaki,Kenji Matsuno

Small (Weinheim an der Bergstrasse, Germany) 19:e2204031 PubMed36635060

2023

Efficient Delivery of GSDMD-N mRNA by Engineered Extracellular Vesicles Induces Pyroptosis for Enhanced Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Xing,Feiyu Zhang,Panpan Ji,Mengying Wei,Chunhui Yin,Angang Yang,Guodong Yang,Jing Zhao

Journal of cell science 135: PubMed36444566

2022

Functions of neuronal Synaptobrevin in the post-Golgi transport of Rhodopsin in Drosophila photoreceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Hitomi Yamashita,Yuka Ochi,Yumi Yamada,Shogo Sasaki,Tatsuya Tago,Takunori Satoh,Akiko K Satoh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker

Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (94)

Product promise