Rabbit Polyclonal GM130 antibody. cis-Golgi marker. Suitable for WB, ICC/IF and reacts with Drosophila melanogaster, Human samples. Cited in 86 publications.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
WB | ICC/IF | |
---|---|---|
Human | Tested | Expected |
Drosophila melanogaster | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Drosophila melanogaster | Dilution info 0.1 µg/mL | Notes - |
Species Human | Dilution info 0.1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Drosophila melanogaster | Dilution info - | Notes - |
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Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed:16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).
Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, Golgin-95, GM130, GOLGA2
Rabbit Polyclonal GM130 antibody. cis-Golgi marker. Suitable for WB, ICC/IF and reacts with Drosophila melanogaster, Human samples. Cited in 86 publications.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
GM130 also known as Golgi matrix protein 130 kDa or GM-130 plays an important role in the organization of the Golgi apparatus. The GM130 protein is vital for maintaining the structure of the Golgi serving as a Golgi marker for researchers. Its molecular weight is approximately 130 kDa. You will commonly find GM130 expressed in cells localizing to the cis-Golgi network. As a Golgi marker it serves as an indication of the integrity and positioning of the Golgi apparatus in the cell.
GM130 serves as a structural component within the Golgi matrix. It interacts with other proteins such as GRASP65 to form a complex which is essential for Golgi stacking and maintenance of Golgi architecture. This interaction helps regulate transport processes inside cells. GM130 proteins help formation of mini-stacks and transitioning of vesicles. In cells GM130 plays a significant role to maintain efficient protein trafficking through the Golgi network.
GM130 interacts with proteins like Rab1 during the vesicular transport pathway. It plays a part in the transport of proteins from the endoplasmic reticulum to the Golgi apparatus. Another related pathway involves SNARE proteins whereby GM130 contributes to membrane docking and fusion events. Through these interactions GM130 modulates the fidelity and specificity of molecular cargo transport within the cell.
GM130 has a connection to neurodegenerative diseases such as frontotemporal dementia. Disruption in GM130 proteins affects Golgi structure and function potentially leading to protein trafficking defects associated with the disorder. GM130 also relates to Alzheimer's disease through its interaction with tau protein. Alterations in GM130's function can influence tau pathology contributing to disease progression.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.
Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1.
The image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.
All lanes: Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/mL
Lane 1: Drosophila melanogaster S2 cell extract
Lane 2: Drosophila melanogaster S2 cell extract + specific siRNA
Predicted band size: 113 kDa
Observed band size: 95 kDa
All lanes: Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/mL
Lane 1: Schneider L2 whole cell lysate (ab14893) at 10 µg
Lane 2: Schneider L2 whole cell lysate (ab14893) at 10 µg with D. melanogaster GM130 peptide (ab32335)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Predicted band size: 113 kDa
Observed band size: 105 kDa, 70 kDa
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