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Chicken Recombinant Monoclonal GM130 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - Chicken IgY (Chimeric) - BSA and Azide free (AB302490), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - Chicken IgY (Chimeric) - BSA and Azide free (AB302490), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - Chicken IgY (Chimeric) - BSA and Azide free (AB302490), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-GM130 antibody [EP892Y] - Chicken IgY (Chimeric) - BSA and Azide free (AB302490), expandable thumbnail

Key facts

Isotype
IgY
Host species
Chicken
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Not recommended
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Version

Target data

Function

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed:16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).

Alternative names

Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, Golgin-95, GM130, GOLGA2

Recommended products

Chicken Recombinant Monoclonal GM130 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgY
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP892Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab302490 is a carrier free version of Anti-GM130 antibody [EP892Y] - Chicken IgY (Chimeric) ab302489.

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab52649). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GM130 also known as Golgi matrix protein 130 kDa or GM-130 plays an important role in the organization of the Golgi apparatus. The GM130 protein is vital for maintaining the structure of the Golgi serving as a Golgi marker for researchers. Its molecular weight is approximately 130 kDa. You will commonly find GM130 expressed in cells localizing to the cis-Golgi network. As a Golgi marker it serves as an indication of the integrity and positioning of the Golgi apparatus in the cell.

Biological function summary

GM130 serves as a structural component within the Golgi matrix. It interacts with other proteins such as GRASP65 to form a complex which is essential for Golgi stacking and maintenance of Golgi architecture. This interaction helps regulate transport processes inside cells. GM130 proteins help formation of mini-stacks and transitioning of vesicles. In cells GM130 plays a significant role to maintain efficient protein trafficking through the Golgi network.

Pathways

GM130 interacts with proteins like Rab1 during the vesicular transport pathway. It plays a part in the transport of proteins from the endoplasmic reticulum to the Golgi apparatus. Another related pathway involves SNARE proteins whereby GM130 contributes to membrane docking and fusion events. Through these interactions GM130 modulates the fidelity and specificity of molecular cargo transport within the cell.

Associated diseases and disorders

GM130 has a connection to neurodegenerative diseases such as frontotemporal dementia. Disruption in GM130 proteins affects Golgi structure and function potentially leading to protein trafficking defects associated with the disorder. GM130 also relates to Alzheimer's disease through its interaction with tau protein. Alterations in GM130's function can influence tau pathology contributing to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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