Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) is a rabbit monoclonal antibody detecting GM130 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for African green monkey, Dog, Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 290 publications
- Trusted since 2007
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
African green monkey | Expected | Tested | Expected | Expected | Expected |
Cow | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Dog | Expected | Tested | Expected | Expected | Expected |
Monkey | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 - 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Dog | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Overnight incubation is recommended. |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Overnight incubation is recommended. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Overnight incubation is recommended. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Cow | Dilution info - | Notes - |
Species Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/250 | Notes PFA fixation should be most suitable. |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes PFA fixation should be most suitable. |
Species Rat | Dilution info - | Notes PFA fixation should be most suitable. |
Species Cow | Dilution info - | Notes - |
Species Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Monkey | Dilution info - | Notes - |
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Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed:16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).
Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, Golgin-95, GM130, GOLGA2
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) is a rabbit monoclonal antibody detecting GM130 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for African green monkey, Dog, Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 290 publications
- Trusted since 2007
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Mouse and rat cell lines pc12, 3t3, raw 264.7 were tested positive in WB. However, brain, kidney, spleen and heart were negative from the two species.
Product Specifications
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in african green monkey, dog, human samples.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) specifically detects GM130 (UniProt ID: Q08379; Molecular weight: 113kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has been cited over 294 times in peer reviewed journals and is trusted by the scientific community.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has 38 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone EP892Y - Anti-GM130 antibody [EP892Y] - BSA and Azide free ab215966.
Antibody clone EP892Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 750 (Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab195303, Alexa Fluor® 488 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab275987, Alexa Fluor® 594 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab277236, PE Anti-GM130 - cis-Golgi Marker antibody [EP892Y] ab303011, APC Anti-GM130 - cis-Golgi Marker antibody [EP892Y] ab303012, HRP Anti-GM130 - cis-Golgi Marker antibody [EP892Y] ab303013, Alkaline Phosphatase Anti-GM130 antibody [EP892Y] ab308664, Alexa Fluor® 555 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab311890, Alexa Fluor® 568 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab312361, Alexa Fluor® 750 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab321664).
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Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
GM130 also known as Golgi matrix protein 130 kDa or GM-130 plays an important role in the organization of the Golgi apparatus. The GM130 protein is vital for maintaining the structure of the Golgi serving as a Golgi marker for researchers. Its molecular weight is approximately 130 kDa. You will commonly find GM130 expressed in cells localizing to the cis-Golgi network. As a Golgi marker it serves as an indication of the integrity and positioning of the Golgi apparatus in the cell.
GM130 serves as a structural component within the Golgi matrix. It interacts with other proteins such as GRASP65 to form a complex which is essential for Golgi stacking and maintenance of Golgi architecture. This interaction helps regulate transport processes inside cells. GM130 proteins help formation of mini-stacks and transitioning of vesicles. In cells GM130 plays a significant role to maintain efficient protein trafficking through the Golgi network.
GM130 interacts with proteins like Rab1 during the vesicular transport pathway. It plays a part in the transport of proteins from the endoplasmic reticulum to the Golgi apparatus. Another related pathway involves SNARE proteins whereby GM130 contributes to membrane docking and fusion events. Through these interactions GM130 modulates the fidelity and specificity of molecular cargo transport within the cell.
GM130 has a connection to neurodegenerative diseases such as frontotemporal dementia. Disruption in GM130 proteins affects Golgi structure and function potentially leading to protein trafficking defects associated with the disorder. GM130 also relates to Alzheimer's disease through its interaction with tau protein. Alterations in GM130's function can influence tau pathology contributing to disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
ab52649 (purified) at 1/20 immunoprecipitating GM130 in HeLa whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) was used for detection (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Lane 1: Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
Lanes 2 - 3: Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/20 dilution
Lane 1: HeLa whole cell lysate at 10 µg
Lane 2: ab52649 + HeLa whole cell lysate at 10 µg
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52649 in HeLa whole cell lysate
Predicted band size: 113 kDa
Observed band size: 130 kDa
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/1000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: MCF7 cell lysate at 20 µg
All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 113 kDa
Observed band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling GM130 with purified ab52649 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/200000 dilution
All lanes: HeLa cell lysate at 10 µg
All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 113 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GM130 with purified ab52649 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/50) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GM130 (red) with ab52649 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/5000 dilution
Lane 1: MDCK(NBL-2) cell lysate at 20 µg
Lane 2: MDCK(BL-1) cell lysate at 20 µg
Lane 3: COS-1 cell lysate at 20 µg
All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 113 kDa
Observed band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GM130 with unpurified ab52649 at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Unpurified ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.
Blocking and dilution buffer: 1XPBS-Tween, 5% milk
Exposure: 10 minutes.
All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/2500 dilution
All lanes: MDCK 2 cells at 25 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 113 kDa
Blocking and dilution buffer: PBS, 0.05% Tween
Exposure: 5 minutes.
All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/1000 dilution
All lanes: COS-7 Cell Line from African green monkey kidney whole cell lysate
Predicted band size: 113 kDa
ICC/IF image of unpurified Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade ab52946, 1μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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