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Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) is a rabbit monoclonal antibody detecting GM130 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for African green monkey, Dog, Human.



- Biophysical QC for unrivalled batch-batch consistency

- Over 290 publications

- Trusted since 2007


Images

Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649), expandable thumbnail
  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649), expandable thumbnail
  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
African green monkey
Expected
Tested
Expected
Expected
Expected
Cow
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Dog
Expected
Tested
Expected
Expected
Expected
Monkey
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/20 - 1/50
Notes

-

Expected
Expected

Species
African green monkey, Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Cow
Dilution info
-
Notes

-

Species
Monkey
Dilution info
-
Notes

-

Tested
Tested

Species
African green monkey
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Dog
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Cow, Monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

Overnight incubation is recommended.

Expected
Expected

Species
African green monkey, Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Overnight incubation is recommended.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Overnight incubation is recommended.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Cow
Dilution info
-
Notes

-

Species
Monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/250
Notes

PFA fixation should be most suitable.

Expected
Expected

Species
African green monkey, Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

PFA fixation should be most suitable.

Species
Rat
Dilution info
-
Notes

PFA fixation should be most suitable.

Species
Cow
Dilution info
-
Notes

-

Species
Monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
African green monkey, Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Cow, Monkey
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed:16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).

Alternative names

Recommended products

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) is a rabbit monoclonal antibody detecting GM130 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for African green monkey, Dog, Human.



- Biophysical QC for unrivalled batch-batch consistency

- Over 290 publications

- Trusted since 2007

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP892Y
Purification technique
Affinity purification Protein A
Specificity

Mouse and rat cell lines pc12, 3t3, raw 264.7 were tested positive in WB. However, brain, kidney, spleen and heart were negative from the two species.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Product Specifications

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)  was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in african green monkey, dog, human samples.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) specifically detects GM130  (UniProt ID: Q08379; Molecular weight: 113kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-GM130 antibody [EP892Y] - cis-Golgi Marker  (ab52649) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has been cited over 294 times in peer reviewed journals and is trusted by the scientific community.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has 38 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone EP892Y - Anti-GM130 antibody [EP892Y] - BSA and Azide free ab215966.
Antibody clone EP892Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 750 (Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab195303, Alexa Fluor® 488 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab275987, Alexa Fluor® 594 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab277236, PE Anti-GM130 - cis-Golgi Marker antibody [EP892Y] ab303011, APC Anti-GM130 - cis-Golgi Marker antibody [EP892Y] ab303012, HRP Anti-GM130 - cis-Golgi Marker antibody [EP892Y] ab303013, Alkaline Phosphatase Anti-GM130 antibody [EP892Y] ab308664, Alexa Fluor® 555 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab311890, Alexa Fluor® 568 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab312361, Alexa Fluor® 750 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker ab321664).

Explore our range of organelle-specific antibodies and dyes and discover why our reagents are deeply trusted by cell biologists.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GM130 also known as Golgi matrix protein 130 kDa or GM-130 plays an important role in the organization of the Golgi apparatus. The GM130 protein is vital for maintaining the structure of the Golgi serving as a Golgi marker for researchers. Its molecular weight is approximately 130 kDa. You will commonly find GM130 expressed in cells localizing to the cis-Golgi network. As a Golgi marker it serves as an indication of the integrity and positioning of the Golgi apparatus in the cell.

Biological function summary

GM130 serves as a structural component within the Golgi matrix. It interacts with other proteins such as GRASP65 to form a complex which is essential for Golgi stacking and maintenance of Golgi architecture. This interaction helps regulate transport processes inside cells. GM130 proteins help formation of mini-stacks and transitioning of vesicles. In cells GM130 plays a significant role to maintain efficient protein trafficking through the Golgi network.

Pathways

GM130 interacts with proteins like Rab1 during the vesicular transport pathway. It plays a part in the transport of proteins from the endoplasmic reticulum to the Golgi apparatus. Another related pathway involves SNARE proteins whereby GM130 contributes to membrane docking and fusion events. Through these interactions GM130 modulates the fidelity and specificity of molecular cargo transport within the cell.

Associated diseases and disorders

GM130 has a connection to neurodegenerative diseases such as frontotemporal dementia. Disruption in GM130 proteins affects Golgi structure and function potentially leading to protein trafficking defects associated with the disorder. GM130 also relates to Alzheimer's disease through its interaction with tau protein. Alterations in GM130's function can influence tau pathology contributing to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

  • Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    ab52649 (purified) at 1/20 immunoprecipitating GM130 in HeLa whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) was used for detection (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    Lane 1: Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Lanes 2 - 3: Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/20 dilution

    Lane 1: HeLa whole cell lysate at 10 µg

    Lane 2: ab52649 + HeLa whole cell lysate at 10 µg

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52649 in HeLa whole cell lysate

    Predicted band size: 113 kDa

    Observed band size: 130 kDa

  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 113 kDa

    Observed band size: 130 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling GM130 with purified ab52649 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/200000 dilution

    All lanes: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 113 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GM130 with purified ab52649 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/50) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry (Intracellular) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GM130 (red) with ab52649 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/5000 dilution

    Lane 1: MDCK(NBL-2) cell lysate at 20 µg

    Lane 2: MDCK(BL-1) cell lysate at 20 µg

    Lane 3: COS-1 cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 113 kDa

    Observed band size: 130 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GM130 with unpurified ab52649 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail
    This image is courtesy of a customer review submitted by Vladimir Milenkovic.

    Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Unpurified ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.

  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Blocking and dilution buffer: 1XPBS-Tween, 5% milk

    Exposure: 10 minutes.

    All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/2500 dilution

    All lanes: MDCK 2 cells at 25 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 113 kDa

  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Blocking and dilution buffer: PBS, 0.05% Tween

    Exposure: 5 minutes.

    All lanes: Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/1000 dilution

    All lanes: COS-7 Cell Line from African green monkey kidney whole cell lysate

    Predicted band size: 113 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    ICC/IF image of unpurified Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade ab52946, 1μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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