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AB315153

Anti-Gm6A antibody [EPR25802-21] - BSA and Azide free

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Rabbit Recombinant Monoclonal Guanine N6-methyladenosine (Gm6A) antibody. Carrier free. Suitable for FRET, Dot and reacts with Nucleic Acid samples.

View Alternative Names

Guanine N6-methyladenosine, Gm6A

3 Images
Dot Blot - Anti-Gm6A antibody [EPR25802-21] - BSA and Azide free (AB315153)
  • Dot

Supplier Data

Dot Blot - Anti-Gm6A antibody [EPR25802-21] - BSA and Azide free (AB315153)

This data was developed using ab315152, the same antibody clone in a different buffer formulation.

Dot blot analysis of Gm6A using ab315152 at 1 : 500 (1ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 20,000 dilution.

All lanes:

Dot Blot - Anti-Gm6A antibody [EPR25802-21] (<a href='/en-us/products/primary-antibodies/gm6a-antibody-epr25802-21-ab315152'>ab315152</a>) at 1/500 dilution

Lane 1:

HeLa total RNA

Lane 2:

HeLa mRNA

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 32s

Fluorescence Resonance Energy Transfer - Anti-Gm6A antibody [EPR25802-21] - BSA and Azide free (AB315153)
  • FRET

Supplier Data

Fluorescence Resonance Energy Transfer - Anti-Gm6A antibody [EPR25802-21] - BSA and Azide free (AB315153)

This data was developed using ab315152, the same antibody clone in a different buffer formulation.

A FRET-based assay was performed using 25nm of each biotinylated oligos and ab315152 at a concentration range of 0.001-4.000 µg/mL

Dot Blot - Anti-Gm6A antibody [EPR25802-21] - BSA and Azide free (AB315153)
  • Dot

Supplier Data

Dot Blot - Anti-Gm6A antibody [EPR25802-21] - BSA and Azide free (AB315153)

This data was developed using ab315152, the same antibody clone in a different buffer formulation.

Dot blot analysis of Gm6A using ab315152 at 1 : 500 (1ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 20,000 dilution.

All lanes:

Dot Blot - Anti-Gm6A antibody [EPR25802-21] (<a href='/en-us/products/primary-antibodies/gm6a-antibody-epr25802-21-ab315152'>ab315152</a>) at 1/500 dilution

Lane 1:

A-oligo

Lane 2:

Am-oligo

Lane 3:

m6A-oligo

Lane 4:

m6, 6A-oligo

Lane 5:

m6Am-oligo

Lane 6:

G-oligo

Lane 7:

Gm6A-oligo

Lane 8:

Am6A-oligo

Lane 9:

Cm6A-oligo

Lane 10:

m8A-oligo

Lane 11:

m1A-oligo

Lane 12:

m1G-oligo

Lane 13:

m2G-oligo

Lane 14:

m2, 2G-oligo

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25802-21

Isotype

IgG

Carrier free

Yes

Applications

FRET, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FRET" : {"fullname" : "Fluorescence Resonance Energy Transfer", "shortname":"FRET"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Nucleic Acid": { "FRET-species-checked": "testedAndGuaranteed", "FRET-species-dilution-info": "", "FRET-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }
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Product details

ab315153 is the carrier-free version of ab315152.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gm6A also known as N6-methyladenosine (m6A) methyltransferase is an essential component of the RNA modification machinery. It plays a critical role in post-transcriptional regulation. The protein mass of Gm6A is approximately 86 kDa. It is widely expressed in mammalian cells particularly prominent in brain and liver tissues. Gm6A facilitates the methylation of adenosine residues on RNA molecules influencing RNA stability splicing and export.
Biological function summary

Gm6A functions in the regulation of RNA metabolism. As an important player in epitranscriptomics it is part of the methyltransferase complex along with METTL3 and METTL14. This complex modifies messenger RNA to control gene expression and ensure proper cellular responses to various stimuli. The modification process governed by Gm6A is essential for maintaining RNA dynamic functions involving feedback loops where altered RNA impacts protein synthesis and cell signaling pathways.

Pathways

Multiple cellular processes rely on the functions of Gm6A methylation. It's involved in the mRNA processing pathway contributing to splicing and alternative polyadenylation. Gm6A interacts with proteins like YTHDF1 and YTHDF2 which mediate mRNA translation and degradation. Through these pathways Gm6A supports cell differentiation and stress responses embedding itself in the larger network of gene expression regulation.

Gm6A regulation has implications in cancer and neurological disorders. Altered m6A methylation patterns correlate with tumorigenesis in cancers including leukemia and glioblastoma. The aberrant methylation affects proteins like FTO which is an m6A demethylase potentially driving oncogenic pathways. In neurological contexts disruptions in m6A methylation play a role in neurodevelopmental disorders with implications for proteins such as RBM15 that are involved in cognitive functions and brain development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Guanine N6-methyladenosine (Gm6A)
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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