Rabbit Polyclonal GMAP-210 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TRIP11 aa 1-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TRIP11 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q15643
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Target data
Function
Is a membrane tether required for vesicle tethering to Golgi. Has an essential role in the maintenance of Golgi structure and function (PubMed:25473115, PubMed:30728324). It is required for efficient anterograde and retrograde trafficking in the early secretory pathway, functioning at both the ER-to-Golgi intermediate compartment (ERGIC) and Golgi complex (PubMed:25717001). Binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription.
Alternative names
CEV14, TRIP11, Thyroid receptor-interacting protein 11, TR-interacting protein 11, TRIP-11, Clonal evolution-related gene on chromosome 14 protein, Golgi-associated microtubule-binding protein 210, Trip230, GMAP-210
Recommended products
Rabbit Polyclonal GMAP-210 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TRIP11 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TRIP11 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q15643
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GMAP-210 also known as Trip11 is a large coiled-coil protein with a molecular weight of approximately 228 kDa. It primarily functions as a Golgi microtubule-associated protein playing a role in the structural integrity and organization of the Golgi apparatus. This protein is expressed in cells across various tissues highlighting its important role in maintaining proper cellular operations.
- Biological function summary
GMAP-210 assists in vesicle tethering and trafficking processes ensuring the effective movement of proteins and lipids along the secretory pathway. It is not part of any large protein complex but interacts with other Golgi-associated proteins to stabilize microtubules and Golgi membranes. Its interactions are key for the correct sorting and delivery of proteins to their destinations within cells making it integral to maintaining cellular membrane dynamics.
- Pathways
GMAP-210 functions significantly within the secretory pathway. It interacts with proteins essential for Golgi transport such as Golgin-84 to mediate the movement of vesicles between the Golgi stacks and other cellular compartments. Additionally GMAP-210 has roles in the regulation of the microtubule network which is vital for intracellular transport and cellular morphology maintenance.
- Associated diseases and disorders
Defects in the GMAP-210 protein are linked to achondrogenesis type 1A and can also contribute to other skeletal dysplasias. It is associated with collagen processing errors due to its role in Golgi transport which can lead to structural protein accumulation. These disorders highlight the importance of GMAP-210's interaction with proteins like collagen in maintaining normal skeletal development and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-GMAP-210 antibody (ab251664)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for GMAP-210 (green) using ab251664 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMAP-210 antibody (ab251664)
Paraffin-embedded human pancreas tissue stained for GMAP-210 using ab251664 at 1/50 dilution in immunohistochemical analysis.
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