AB72223

Anti-GMAP-210 antibody

Name: Anti-GMAP-210 antibody (ab72223) | Abcam
Brand: Abcam Limited
SKU: AB72223

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(1 Publication)

Rabbit Polyclonal GMAP-210 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TRIP11 aa 1-100.

View Alternative Names

CEV14, TRIP11, Thyroid receptor-interacting protein 11, TR-interacting protein 11, TRIP-11, Clonal evolution-related gene on chromosome 14 protein, Golgi-associated microtubule-binding protein 210, Trip230, GMAP-210

2 Images

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Immunoprecipitation - Anti-GMAP-210 antibody (AB72223)

Detection of Human GMAP-210 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with ab72223 at 3μg in lane 1, an antibody recognising a downstream epitope in lane 2 and control IgG in lane 3. Image shows immunoprecipitated GMAP-210 detected with post IP WB, loading 20% of IP and using ab72223 at 0.1 μg/ml. Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-GMAP-210 antibody (ab72223)

Predicted band size: 228 kDa

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Unknown

Western blot - Anti-GMAP-210 antibody (AB72223)

All lanes:

Western blot - Anti-GMAP-210 antibody (ab72223) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 228 kDa

Observed band size: 238 kDa

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Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human TRIP11 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q15643

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72223 was affinity purified using an epitope specific to GMAP-210 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GMAP-210 also known as Trip11 is a large coiled-coil protein with a molecular weight of approximately 228 kDa. It primarily functions as a Golgi microtubule-associated protein playing a role in the structural integrity and organization of the Golgi apparatus. This protein is expressed in cells across various tissues highlighting its important role in maintaining proper cellular operations.

Biological function summary

GMAP-210 assists in vesicle tethering and trafficking processes ensuring the effective movement of proteins and lipids along the secretory pathway. It is not part of any large protein complex but interacts with other Golgi-associated proteins to stabilize microtubules and Golgi membranes. Its interactions are key for the correct sorting and delivery of proteins to their destinations within cells making it integral to maintaining cellular membrane dynamics.

Pathways

GMAP-210 functions significantly within the secretory pathway. It interacts with proteins essential for Golgi transport such as Golgin-84 to mediate the movement of vesicles between the Golgi stacks and other cellular compartments. Additionally GMAP-210 has roles in the regulation of the microtubule network which is vital for intracellular transport and cellular morphology maintenance.

Defects in the GMAP-210 protein are linked to achondrogenesis type 1A and can also contribute to other skeletal dysplasias. It is associated with collagen processing errors due to its role in Golgi transport which can lead to structural protein accumulation. These disorders highlight the importance of GMAP-210's interaction with proteins like collagen in maintaining normal skeletal development and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Is a membrane tether required for vesicle tethering to Golgi. Has an essential role in the maintenance of Golgi structure and function (PubMed : 25473115, PubMed : 30728324). It is required for efficient anterograde and retrograde trafficking in the early secretory pathway, functioning at both the ER-to-Golgi intermediate compartment (ERGIC) and Golgi complex (PubMed : 25717001). Binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription.

See full target information TRIP11

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 288:10286-97 PubMed23439653

2013

Constitutive internalization of the leucine-rich G protein-coupled receptor-5 (LGR5) to the trans-Golgi network.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua C Snyder,Lauren K Rochelle,H Kim Lyerly,Marc G Caron,Lawrence S Barak

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GMAP-210 antibody

Immunoprecipitation - Anti-GMAP-210 antibody (AB72223)

Western blot - Anti-GMAP-210 antibody (AB72223)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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