Rabbit Polyclonal GMFB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GMFB aa 1 to C-terminus.
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Publications
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- Molecular neurobiology 56:378-3932018Targeted Gene Editing of Glia Maturation Factor in Microglia: a Novel Alzheimer's Disease Therapeutic Target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSudhanshu P Raikwar et. al.PubMed 29704201
- Journal of Alzheimer's disease : JAD 61:553-5602017Co-Expression of Glia Maturation Factor and Apolipoprotein E4 in Alzheimer's Disease Brain.Applications:IHC-PReactive species:Unspecified reactive speciesRamasamy Thangavel,Sachin M Bhagavan,Swathi Beladakere Ramaswamy,Spurthi Surpur,Raghav Govindarajan,Duraisamy Kempuraj,Smita Zaheer,Sudhanshu Raikwar,Mohammad E Ahmed,Govindhasamy Pushpavathi Selvakumar,Shankar S Iyer,Asgar ZaheerPubMed 29172001
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GMFB aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P60983
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Target data
Function
This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells.
Alternative names
Glia maturation factor beta, GMF-beta, GMFB
Recommended products
Rabbit Polyclonal GMFB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GMFB aa 1 to C-terminus.
Alternative names
Glia maturation factor beta, GMF-beta, GMFB
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GMFB aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P60983
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Glia Maturation Factor Beta (GMFB) is an important regulator in brain development and maintenance. It is also known by the name GMF-b. This protein weighs approximately 17.2 kDa and is expressed in glial cells of the central nervous system as well as other tissues including skin and kidney. By stimulating differentiation and maturation of glial cells GMFB influences central nervous system homeostasis and glial responses to injury.
- Biological function summary
GMFB influences numerous cellular processes such as brain maturation and injury repair. It does not form part of a complex but functions independently to exert influence on various targets. GMFB aids in the reorganization of the actin cytoskeleton which is important for cell shape and motility. It regulates neural signaling by interacting with signaling molecules enabling adaptability and cellular communication.
- Pathways
GMFB significantly affects neurotrophic and inflammatory pathways. It interacts with proteins like PI3K and AKT to modulate neural survival and plasticity impacting brain health and response to stress. The protein also involves itself in inflammation-related pathways supporting cytokine production and immune responses during injury and infection. Through these interactions GMFB ensures optimal cellular function and organismal health.
- Associated diseases and disorders
GMFB has links to neurodegenerative diseases and gliomas. Abnormal GMFB expression connects with Alzheimer's disease affecting neural health and functionality. In the context of gliomas GMFB influences tumor progression and microenvironment implicating it in tumorigenesis. In these disorders GMFB interacts with proteins such as GFAP and beta-amyloid leading to dysregulation in cellular signaling and pathological developments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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12 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)
Paraffin-embedded mouse cerebellum tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-GMFB antibody (ab224322)
PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for GMFB (green) using ab224322 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)
Paraffin-embedded human hippocampus tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)
Paraffin-embedded mouse red nucleus tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
Western blot - Anti-GMFB antibody (ab224322)
All lanes: Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution
All lanes: A431 (human epidermoid carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 17 kDa
Western blot - Anti-GMFB antibody (ab224322)
All lanes: Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution
All lanes: Human cerebral cortex tissue lysate
Developed using the ECL technique.
Predicted band size: 17 kDa
Western blot - Anti-GMFB antibody (ab224322)
All lanes: Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2: NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 17 kDa
Western blot - Anti-GMFB antibody (ab224322)
All lanes: Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution
All lanes: Mouse cerebral cortex tissue lysate
Developed using the ECL technique.
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)
Paraffin-embedded mouse lateral septum tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)
Paraffin-embedded mouse olfactory bulb tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)
Paraffin-embedded human cerebral cortex tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (ab224322)
Paraffin-embedded mouse somatosensory cortex tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.
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