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AB224322

Anti-GMFB antibody

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(3 Publications)

Rabbit Polyclonal GMFB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GMFB aa 1 to C-terminus.

View Alternative Names

Glia maturation factor beta, GMF-beta, GMFB

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded human hippocampus tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GMFB antibody (AB224322)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GMFB antibody (AB224322)

PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for GMFB (green) using ab224322 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded human cerebral cortex tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse lateral septum tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse cerebellum tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse somatosensory cortex tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse olfactory bulb tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse red nucleus tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

Western blot - Anti-GMFB antibody (AB224322)
  • WB

Supplier Data

Western blot - Anti-GMFB antibody (AB224322)

All lanes:

Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution

All lanes:

Human cerebral cortex tissue lysate

Predicted band size: 17 kDa

true

Western blot - Anti-GMFB antibody (AB224322)
  • WB

Supplier Data

Western blot - Anti-GMFB antibody (AB224322)

All lanes:

Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 17 kDa

true

Western blot - Anti-GMFB antibody (AB224322)
  • WB

Supplier Data

Western blot - Anti-GMFB antibody (AB224322)

All lanes:

Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 17 kDa

true

Western blot - Anti-GMFB antibody (AB224322)
  • WB

Supplier Data

Western blot - Anti-GMFB antibody (AB224322)

All lanes:

Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution

All lanes:

Mouse cerebral cortex tissue lysate

Predicted band size: 17 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GMFB aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P60983

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Glia Maturation Factor Beta (GMFB) is an important regulator in brain development and maintenance. It is also known by the name GMF-b. This protein weighs approximately 17.2 kDa and is expressed in glial cells of the central nervous system as well as other tissues including skin and kidney. By stimulating differentiation and maturation of glial cells GMFB influences central nervous system homeostasis and glial responses to injury.
Biological function summary

GMFB influences numerous cellular processes such as brain maturation and injury repair. It does not form part of a complex but functions independently to exert influence on various targets. GMFB aids in the reorganization of the actin cytoskeleton which is important for cell shape and motility. It regulates neural signaling by interacting with signaling molecules enabling adaptability and cellular communication.

Pathways

GMFB significantly affects neurotrophic and inflammatory pathways. It interacts with proteins like PI3K and AKT to modulate neural survival and plasticity impacting brain health and response to stress. The protein also involves itself in inflammation-related pathways supporting cytokine production and immune responses during injury and infection. Through these interactions GMFB ensures optimal cellular function and organismal health.

GMFB has links to neurodegenerative diseases and gliomas. Abnormal GMFB expression connects with Alzheimer's disease affecting neural health and functionality. In the context of gliomas GMFB influences tumor progression and microenvironment implicating it in tumorigenesis. In these disorders GMFB interacts with proteins such as GFAP and beta-amyloid leading to dysregulation in cellular signaling and pathological developments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells.
See full target information GMFB
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 20:177 PubMed37507761

2023

Cellulose ether treatment inhibits amyloid beta aggregation, neuroinflammation and cognitive deficits in transgenic mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tahir Ali,Antonia N Klein,Keegan McDonald,Lovisa Johansson,Priyanka Ganguli Mukherjee,Martin Hallbeck,Katsumi Doh-Ura,Hermann M Schatzl,Sabine Gilch

Molecular neurobiology 56:378-393 PubMed29704201

2018

Targeted Gene Editing of Glia Maturation Factor in Microglia: a Novel Alzheimer's Disease Therapeutic Target.

Applications

Unspecified application

Species

Unspecified reactive species

Sudhanshu P Raikwar,Ramasamy Thangavel,Iuliia Dubova,Govindhasamy Pushpavathi Selvakumar,Mohammad Ejaz Ahmed,Duraisamy Kempuraj,Smita A Zaheer,Shankar S Iyer,Asgar Zaheer

Journal of Alzheimer's disease : JAD 61:553-560 PubMed29172001

2017

Co-Expression of Glia Maturation Factor and Apolipoprotein E4 in Alzheimer's Disease Brain.

Applications

IHC-P

Species

Unspecified reactive species

Ramasamy Thangavel,Sachin M Bhagavan,Swathi Beladakere Ramaswamy,Spurthi Surpur,Raghav Govindarajan,Duraisamy Kempuraj,Smita Zaheer,Sudhanshu Raikwar,Mohammad E Ahmed,Govindhasamy Pushpavathi Selvakumar,Shankar S Iyer,Asgar Zaheer
View all publications
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Product promise

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