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AB303512

Anti-GMFB antibody [EPR25849-25]

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(1 Publication)

Rabbit Recombinant Monoclonal GMFB antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Glia maturation factor beta, GMF-beta, GMFB

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GMFB antibody [EPR25849-25] (AB303512)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GMFB antibody [EPR25849-25] (AB303512)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labelling GMFB with ab303512 at 1/50 (10.98 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and nuclear staining in U-87 MG cell line. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-GMFB antibody [EPR25849-25] (AB303512)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GMFB antibody [EPR25849-25] (AB303512)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labelling GMFB with ab303512 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-GMFB antibody [EPR25849-25] (AB303512)
  • WB

Supplier Data

Western blot - Anti-GMFB antibody [EPR25849-25] (AB303512)

Blocking / Diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human GMFG.

All lanes:

Western blot - Anti-GMFB antibody [EPR25849-25] (ab303512) at 1/1000 dilution

Lane 1:

His-tagged human GMFB recombinant protein at 10 ng

Lane 2:

His-tagged human GMFG recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Exposure time: 15s

  • Carrier free

    Anti-GMFB antibody [EPR25849-25] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25849-25

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Glia Maturation Factor Beta (GMFB) is an important regulator in brain development and maintenance. It is also known by the name GMF-b. This protein weighs approximately 17.2 kDa and is expressed in glial cells of the central nervous system as well as other tissues including skin and kidney. By stimulating differentiation and maturation of glial cells GMFB influences central nervous system homeostasis and glial responses to injury.
Biological function summary

GMFB influences numerous cellular processes such as brain maturation and injury repair. It does not form part of a complex but functions independently to exert influence on various targets. GMFB aids in the reorganization of the actin cytoskeleton which is important for cell shape and motility. It regulates neural signaling by interacting with signaling molecules enabling adaptability and cellular communication.

Pathways

GMFB significantly affects neurotrophic and inflammatory pathways. It interacts with proteins like PI3K and AKT to modulate neural survival and plasticity impacting brain health and response to stress. The protein also involves itself in inflammation-related pathways supporting cytokine production and immune responses during injury and infection. Through these interactions GMFB ensures optimal cellular function and organismal health.

GMFB has links to neurodegenerative diseases and gliomas. Abnormal GMFB expression connects with Alzheimer's disease affecting neural health and functionality. In the context of gliomas GMFB influences tumor progression and microenvironment implicating it in tumorigenesis. In these disorders GMFB interacts with proteins such as GFAP and beta-amyloid leading to dysregulation in cellular signaling and pathological developments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells.
See full target information GMFB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 25:578-587 PubMed39388704

2024

Curcumin ameliorates ischemic stroke injury by downregulating GMFB expression: An study.

Applications

Unspecified application

Species

Unspecified reactive species

Xiumei Bai,Yabin Song,Xiangyan Zhang,Liqiong Liu,Haixia Wu,Jiaqing Feng,Lihong Wu,Huizhen Liu,Diangui Zhou
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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