Rabbit Polyclonal GMPPB antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GMPPB aa 1-250.
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Publications
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- Brain : a journal of neurology 138:2493-5042015Mutations in GMPPB cause congenital myasthenic syndrome and bridge myasthenic disorders with dystroglycanopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatsiaryna Belaya et. al.PubMed 26133662
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GMPPB aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5P6
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Catalytic subunit of the GMPPA-GMPPB mannose-1-phosphate guanylyltransferase complex (PubMed:33986552). Catalyzes the formation of GDP-mannose, an essential precursor of glycan moieties of glycoproteins and glycolipids (PubMed:33986552). Can catalyze the reverse reaction in vitro (PubMed:33986552). Together with GMPPA regulates GDP-alpha-D-mannose levels (PubMed:33986552).
Alternative names
Mannose-1-phosphate guanylyltransferase catalytic subunit beta, GDP-mannose pyrophosphorylase B, GTP-mannose-1-phosphate guanylyltransferase beta, GMPPB
Recommended products
Rabbit Polyclonal GMPPB antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GMPPB aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GMPPB aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5P6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GMPPB also known as GDP-mannose pyrophosphorylase B is an enzyme that catalyzes the conversion of mannose-1-phosphate and GTP into GDP-mannose. This enzyme has a molecular mass of approximately 38 kDa. GMPPB is expressed in various tissues throughout the body with significant levels in skeletal muscle and the brain. It is an essential enzyme for the proper synthesis of nucleotide sugars which play an important role in glycosylation processes.
- Biological function summary
The activity of GMPPB influences the assembly and stability of glycoproteins by providing the necessary substrates for glycosylation. Glycosylation is significant for protein folding stability and cell-cell interactions. GMPPB does not function alone; it is involved in synergy with other enzymes in the GDP-mannose biosynthesis pathway. This pathway is important for the formation of glycosylated proteins and lipids affecting numerous cellular processes and signaling pathways.
- Pathways
GMPPB is actively involved in the nucleotide sugar metabolism and protein glycosylation pathways. It collaborates with other enzymes like ALG1 and ALG2 which further facilitate the glycosylation processes in the endoplasmic reticulum. These pathways are integral for maintaining cellular communication and structural integrity ultimately impacting cellular homeostasis and function.
- Associated diseases and disorders
GMPPB mutations have been linked to congenital disorders of glycosylation and muscular dystrophies such as muscular dystrophy-dystroglycanopathy (MDDG). These conditions involve defective glycosylation leading to improper muscle and neural development and function. Abnormalities in proteins like dystrophin are associated with GMPPB mutations underlining their involvement in these genetic disorders. Understanding GMPPB's role provides insights into potential therapeutic targets for addressing these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-GMPPB antibody (ab154061)
10% SDS PAGE
All lanes: Western blot - Anti-GMPPB antibody (ab154061) at 1/1000 dilution
All lanes: IMR32 whole cell lysate at 30 µg
Predicted band size: 40 kDa
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