AB153951

Anti-GNA11 antibody

Name: Anti-GNA11 antibody (ab153951) | Abcam
Brand: Abcam Limited
SKU: AB153951

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(4 Publications)

Rabbit Polyclonal GNA11 antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GNA11 aa 150 to C-terminus.

View Alternative Names

GA11, GNA11, Guanine nucleotide-binding protein subunit alpha-11, G alpha-11, G-protein subunit alpha-11, Guanine nucleotide-binding protein G(y) subunit alpha

2 Images

Supplier Data

Western blot - Anti-GNA11 antibody (AB153951)

All lanes:

Western blot - Anti-GNA11 antibody (ab153951) at 1/1000 dilution

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Rat brain tissue extract at 50 µg

Predicted band size: 42 kDa

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Supplier Data

Western blot - Anti-GNA11 antibody (AB153951)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-GNA11 antibody (ab153951) at 1/1000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human GNA11 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29992

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNA11 also known as G-protein subunit alpha-11 is a protein that functions mechanically within cells as part of the G protein family. This protein plays a role in transmitting signals from the surface of the cell to its interior. GNA11 has a molecular mass of approximately 42 kDa and it is mainly expressed in various tissues including the heart and central nervous system. It operates by interacting with receptors on the cell surface instigating conformational changes that initiate intracellular signaling cascades.

Biological function summary

G-protein subunit alpha-11 interacts with several signaling molecules and works predominantly as part of a heterotrimeric G protein complex. This protein complex plays an important role in modulating a variety of biological processes such as cardiovascular function and neuronal signaling. GNA11 contributes to cellular responses by activating downstream effectors which can influence cellular growth differentiation and response to external stimuli.

Pathways

G-protein subunit alpha-11 is an essential component of the phosphoinositide signaling pathway. It assists in the conversion of extracellular signals into intracellular action by facilitating the release of secondary messengers like inositol trisphosphate. In this pathway it works closely with other proteins like GNAQ and GNA15 which share similar mechanistic roles. Through these interactions GNA11 regulates calcium mobilization and protein kinase C activity impacting various cellular functions.

GNA11 mutations have links to certain conditions like uveal melanoma and hypocalcemia. These mutations alter the normal signaling pathways and can lead to unregulated cell growth as seen in cancer. Uveal melanoma relates to GNA11 through mutations that activate signaling pathways uncontrollably. Similarly in hypocalcemia the GNA11 protein associates with the calcium-sensing receptor which causes imbalances in calcium homeostasis. These associations highlight the significance of GNA11 in pathology as well as signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 31073061). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 31073061). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 31073061). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 31073061). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 31073061). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation : activates phospholipase C-beta : following GPCR activation, GNA11 activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 31073061). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed : 27852822). Together with GNAQ, required for heart development (By similarity).

See full target information GNA11

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 150: PubMed37102682

2023

Stretch regulates alveologenesis and homeostasis via mesenchymal Gαq/11-mediated TGFβ2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda T Goodwin,Alison E John,Chitra Joseph,Anthony Habgood,Amanda L Tatler,Katalin Susztak,Matthew Palmer,Stefan Offermanns,Neil C Henderson,R Gisli Jenkins

Cell & bioscience 13:51 PubMed36895027

2023

GPR116 receptor regulates the antitumor function of NK cells via Gαq/HIF1α/NF-κB signaling pathway as a potential immune checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Guo,Chenxu Jin,Yaoxin Gao,Haizhen Lin,Li Zhang,Ying Zhou,Jie Yao,Yixin Duan,Yaojun Ren,Xinhui Hui,Yujia Ge,Renzheng Yang,Wenzheng Jiang

Arteriosclerosis, thrombosis, and vascular biology 40:1256-1274 PubMed32160773

2020

Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Suresh Govatati,Prahalathan Pichavaram,Jagadeesh Janjanam,Liang Guo,Renu Virmani,Gadiparthi N Rao

Arteriosclerosis, thrombosis, and vascular biology 39:1212-1226 PubMed31043075

2019

NFATc1-E2F1-LMCD1-Mediated IL-33 Expression by Thrombin Is Required for Injury-Induced Neointima Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Suresh Govatati,Prahalathan Pichavaram,Jagadeesh Janjanam,Baolin Zhang,Nikhlesh K Singh,Arul M Mani,James G Traylor,A Wayne Orr,Gadiparthi N Rao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GNA11 antibody

Western blot - Anti-GNA11 antibody (AB153951)

Western blot - Anti-GNA11 antibody (AB153951)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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