Rabbit Polyclonal GNA11 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GNA11 aa 150 to C-terminus.
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- Development (Cambridge, England) 150:2023Stretch regulates alveologenesis and homeostasis via mesenchymal Gαq/11-mediated TGFβ2 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda T Goodwin,Alison E John,Chitra Joseph,Anthony Habgood,Amanda L Tatler,Katalin Susztak,Matthew Palmer,Stefan Offermanns,Neil C Henderson,R Gisli JenkinsPubMed 37102682
- Cell & bioscience 13:512023GPR116 receptor regulates the antitumor function of NK cells via Gαq/HIF1α/NF-κB signaling pathway as a potential immune checkpoint.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDandan Guo,Chenxu Jin,Yaoxin Gao,Haizhen Lin,Li Zhang,Ying Zhou,Jie Yao,Yixin Duan,Yaojun Ren,Xinhui Hui,Yujia Ge,Renzheng Yang,Wenzheng JiangPubMed 36895027
- Arteriosclerosis, thrombosis, and vascular biology 40:1256-12742020Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular Lesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuresh Govatati et. al.PubMed 32160773
- Arteriosclerosis, thrombosis, and vascular biology 39:1212-12262019NFATc1-E2F1-LMCD1-Mediated IL-33 Expression by Thrombin Is Required for Injury-Induced Neointima Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuresh Govatati,Prahalathan Pichavaram,Jagadeesh Janjanam,Baolin Zhang,Nikhlesh K Singh,Arul M Mani,James G Traylor,A Wayne Orr,Gadiparthi N RaoPubMed 31043075
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GNA11 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29992
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Chicken | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Pig | Predicted |
| Xenopus laevis | Predicted |
| Xenopus tropicalis | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed:31073061). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:31073061). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:31073061). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed:31073061). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:31073061). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation: activates phospholipase C-beta: following GPCR activation, GNA11 activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:31073061). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed:27852822). Together with GNAQ, required for heart development (By similarity).
Alternative names
GA11, GNA11, Guanine nucleotide-binding protein subunit alpha-11, G alpha-11, G-protein subunit alpha-11, Guanine nucleotide-binding protein G(y) subunit alpha
Recommended products
Rabbit Polyclonal GNA11 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GNA11 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GNA11 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29992
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GNA11 also known as G-protein subunit alpha-11 is a protein that functions mechanically within cells as part of the G protein family. This protein plays a role in transmitting signals from the surface of the cell to its interior. GNA11 has a molecular mass of approximately 42 kDa and it is mainly expressed in various tissues including the heart and central nervous system. It operates by interacting with receptors on the cell surface instigating conformational changes that initiate intracellular signaling cascades.
- Biological function summary
G-protein subunit alpha-11 interacts with several signaling molecules and works predominantly as part of a heterotrimeric G protein complex. This protein complex plays an important role in modulating a variety of biological processes such as cardiovascular function and neuronal signaling. GNA11 contributes to cellular responses by activating downstream effectors which can influence cellular growth differentiation and response to external stimuli.
- Pathways
G-protein subunit alpha-11 is an essential component of the phosphoinositide signaling pathway. It assists in the conversion of extracellular signals into intracellular action by facilitating the release of secondary messengers like inositol trisphosphate. In this pathway it works closely with other proteins like GNAQ and GNA15 which share similar mechanistic roles. Through these interactions GNA11 regulates calcium mobilization and protein kinase C activity impacting various cellular functions.
- Associated diseases and disorders
GNA11 mutations have links to certain conditions like uveal melanoma and hypocalcemia. These mutations alter the normal signaling pathways and can lead to unregulated cell growth as seen in cancer. Uveal melanoma relates to GNA11 through mutations that activate signaling pathways uncontrollably. Similarly in hypocalcemia the GNA11 protein associates with the calcium-sensing receptor which causes imbalances in calcium homeostasis. These associations highlight the significance of GNA11 in pathology as well as signaling pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-GNA11 antibody (ab153951)
10% SDS PAGE
All lanes: Western blot - Anti-GNA11 antibody (ab153951) at 1/1000 dilution
All lanes: NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 42 kDa
Western blot - Anti-GNA11 antibody (ab153951)
10% SDS PAGE
All lanes: Western blot - Anti-GNA11 antibody (ab153951) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 42 kDa, 46 kDa
Western blot - Anti-GNA11 antibody (ab153951)
All lanes: Western blot - Anti-GNA11 antibody (ab153951) at 1/1000 dilution
All lanes: Rat brain tissue extract at 50 µg
Predicted band size: 42 kDa
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