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AB234822

Anti-GNA11 antibody

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(1 Publication)

Rabbit Polyclonal GNA11 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GNA11.

View Alternative Names

GA11, GNA11, Guanine nucleotide-binding protein subunit alpha-11, G alpha-11, G-protein subunit alpha-11, Guanine nucleotide-binding protein G(y) subunit alpha

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-GNA11 antibody (AB234822)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GNA11 antibody (AB234822)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for GNA11 (green) using ab234822 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA11 antibody (AB234822)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA11 antibody (AB234822)

Paraffin-embedded human testis tissue stained for GNA11 using ab234822 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GNA11.

P29992

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNA11 also known as G-protein subunit alpha-11 is a protein that functions mechanically within cells as part of the G protein family. This protein plays a role in transmitting signals from the surface of the cell to its interior. GNA11 has a molecular mass of approximately 42 kDa and it is mainly expressed in various tissues including the heart and central nervous system. It operates by interacting with receptors on the cell surface instigating conformational changes that initiate intracellular signaling cascades.
Biological function summary

G-protein subunit alpha-11 interacts with several signaling molecules and works predominantly as part of a heterotrimeric G protein complex. This protein complex plays an important role in modulating a variety of biological processes such as cardiovascular function and neuronal signaling. GNA11 contributes to cellular responses by activating downstream effectors which can influence cellular growth differentiation and response to external stimuli.

Pathways

G-protein subunit alpha-11 is an essential component of the phosphoinositide signaling pathway. It assists in the conversion of extracellular signals into intracellular action by facilitating the release of secondary messengers like inositol trisphosphate. In this pathway it works closely with other proteins like GNAQ and GNA15 which share similar mechanistic roles. Through these interactions GNA11 regulates calcium mobilization and protein kinase C activity impacting various cellular functions.

GNA11 mutations have links to certain conditions like uveal melanoma and hypocalcemia. These mutations alter the normal signaling pathways and can lead to unregulated cell growth as seen in cancer. Uveal melanoma relates to GNA11 through mutations that activate signaling pathways uncontrollably. Similarly in hypocalcemia the GNA11 protein associates with the calcium-sensing receptor which causes imbalances in calcium homeostasis. These associations highlight the significance of GNA11 in pathology as well as signaling pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 31073061). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 31073061). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 31073061). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 31073061). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 31073061). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation : activates phospholipase C-beta : following GPCR activation, GNA11 activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 31073061). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed : 27852822). Together with GNAQ, required for heart development (By similarity).
See full target information GNA11
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed39352757

2024

Neuropilin-2-expressing breast cancer cells mitigate radiation-induced oxidative stress through nitric oxide signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ayush Kumar,Hira Lal Goel,Christi A Silva,Tao Wang,Yansong Geng,Mengdie Wang,Shivam Goel,Kai Hu,Rui Li,Lihua J Zhu,Jennifer L Clark,Lindsay M Ferreira,Michael A Brehm,Thomas J FitzGerald,Arthur M Mercurio
View all publications
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