JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154004

Anti-GNA12 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal GNA12 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein subunit alpha-12 aa 150 to C-terminus.

View Alternative Names

Guanine nucleotide-binding protein subunit alpha-12, G alpha-12, G-protein subunit alpha-12, GNA12

1 Images
Western blot - Anti-GNA12 antibody (AB154004)
  • WB

Unknown

Western blot - Anti-GNA12 antibody (AB154004)

10% SDS PAGE

All lanes:

Western blot - Anti-GNA12 antibody (ab154004) at 1/2000 dilution

Lane 1:

NT2D1 whole cell lysate at 30 µg

Lane 2:

IMR32 whole cell lysate at 30 µg

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Guanine nucleotide-binding protein subunit alpha-12 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q03113

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNA12 also known as Gα12 is a member of the G protein family. This protein roughly weighs 44 kDa. It is part of the larger family of heterotrimeric G proteins which consist of alpha beta and gamma subunits. GNA12 is present in various tissues throughout the body including the heart lungs and thymus. GNA12 plays a mechanical role by acting as a molecular switch inside cells transmitting signals from activated receptors to target molecules which changes their activity.
Biological function summary

GNA12 regulates cell signaling processes involving growth differentiation and movement. It operates within the G12/G13 class of G protein signaling pathways and sometimes it works forming a complex with other proteins. It has been implicated in cellular interactions with the cytoskeleton and affects the organization of actin filaments. These actions influence the control of cell shape and motility suggesting a role in cellular migration and adhesion phenomena.

Pathways

GNA12 participates in the Rho family of GTPases signaling pathway and the MAPK/ERK pathway both important for cellular response and adaptation. Within these pathways GNA12 activates the Rho guanine nucleotide exchange factors which then influences Rho-dependent processes. It also interacts with other proteins such as GNA13 and RHOA linking it to signal transduction mechanisms that modulate various cellular outcomes including growth and differentiation.

GNA12 links to cancer and pulmonary hypertension. Its role in cancer relates to its influence on cell migration and invasion which are processes involved in cancer metastasis. Studies have shown that changes in GNA12's function can contribute to oncogenic transformation. In pulmonary hypertension altered GNA12 signaling may disrupt normal vascular smooth muscle function contributing to disease progression. The protein connects with RhoA and MAPK/ERK pathway components in these contexts indicating its involvement in the pathology of these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed : 12515866, PubMed : 15240885, PubMed : 15525651, PubMed : 16705036, PubMed : 16787920, PubMed : 17565996, PubMed : 22609986, PubMed : 23762476, PubMed : 27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG) (PubMed : 12515866, PubMed : 15240885, PubMed : 16202387). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (PubMed : 15525651, PubMed : 17565996). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating pro-inflammatory cytokine production (PubMed : 16705036, PubMed : 16787920, PubMed : 23762476, PubMed : 27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed : 11976333, PubMed : 16787920). Together with NAPA promotes CDH5 localization to plasma membrane (PubMed : 15980433). May play a role in the control of cell migration through the TOR signaling cascade (PubMed : 22609986).
See full target information Guanine nucleotide-binding protein subunit alpha-12
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:1967-1984 PubMed40704638

2025

Developmental regulation of GABA receptors and downstream molecules in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Aguado,Rocío Alfaro-Ruiz,María Llanos Martínez-Poyato,Ana Esther Moreno-Martínez,Sebastián García-Madrona,Alberto Roldán-Sastre,Pablo Alonso-Gómez,Miriam Fernández,Ricardo Puertas-Avendaño,Ryuichi Shigemoto,Kirill A Martemyanov,Rafael Luján

Cardiovascular diabetology 23:331 PubMed39252021

2024

MiRNA-132/212 encapsulated by adipose tissue-derived exosomes worsen atherosclerosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Guo,Tong-Tian Zhuang,Chang-Chun Li,Fuxingzi Li,Su-Kang Shan,Ming-Hui Zheng,Qiu-Shuang Xu,Yi Wang,Li-Min Lei,Ke-Xin Tang,Wenlu Ouyang,Jia-Yue Duan,Yun-Yun Wu,Ye-Chi Cao,Muhammad Hasnain Ehsan Ullah,Zhi-Ang Zhou,Xiao Lin,Feng Wu,Feng Xu,Xiao-Bo Liao,Ling-Qing Yuan

Science advances 7:eabj6895 PubMed34788088

2021

Discovery of anti-inflammatory physiological peptides that promote tissue repair by reinforcing epithelial barrier formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yukako Oda,Chisato Takahashi,Shota Harada,Shun Nakamura,Daxiao Sun,Kazumi Kiso,Yuko Urata,Hitoshi Miyachi,Yoshinori Fujiyoshi,Alf Honigmann,Seiichi Uchida,Yasushi Ishihama,Fumiko Toyoshima

Developmental cell 47:439-452.e6 PubMed30318244

2018

A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Bipul R Acharya,Alexander Nestor-Bergmann,Xuan Liang,Shafali Gupta,Kinga Duszyc,Estelle Gauquelin,Guillermo A Gomez,Srikanth Budnar,Philippe Marcq,Oliver E Jensen,Zev Bryant,Alpha S Yap
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB236617

Anti-GNA12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA12 antibody (AB236617)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB128900

Anti-GNA13 antibody [EPR5436]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (AB128900)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

  • 4
  • 23
Primary Antibodies

AB153951

Anti-GNA11 antibody

Western blot - Anti-GNA11 antibody (AB153951)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com