Rabbit Polyclonal GNA12 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein subunit alpha-12 aa 100-300.
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- Developmental cell 47:439-452.e62018A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBipul R Acharya et. al.PubMed 30318244
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Guanine nucleotide-binding protein subunit alpha-12 aa 100-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q03113
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:12515866, PubMed:15240885, PubMed:15525651, PubMed:16705036, PubMed:16787920, PubMed:17565996, PubMed:22609986, PubMed:23762476, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG) (PubMed:12515866, PubMed:15240885, PubMed:16202387). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (PubMed:15525651, PubMed:17565996). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating pro-inflammatory cytokine production (PubMed:16705036, PubMed:16787920, PubMed:23762476, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333, PubMed:16787920). Together with NAPA promotes CDH5 localization to plasma membrane (PubMed:15980433). May play a role in the control of cell migration through the TOR signaling cascade (PubMed:22609986).
Alternative names
Guanine nucleotide-binding protein subunit alpha-12, G alpha-12, G-protein subunit alpha-12, GNA12
Recommended products
Rabbit Polyclonal GNA12 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein subunit alpha-12 aa 100-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Guanine nucleotide-binding protein subunit alpha-12 aa 100-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q03113
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GNA12 also known as Gα12 is a member of the G protein family. This protein roughly weighs 44 kDa. It is part of the larger family of heterotrimeric G proteins which consist of alpha beta and gamma subunits. GNA12 is present in various tissues throughout the body including the heart lungs and thymus. GNA12 plays a mechanical role by acting as a molecular switch inside cells transmitting signals from activated receptors to target molecules which changes their activity.
- Biological function summary
GNA12 regulates cell signaling processes involving growth differentiation and movement. It operates within the G12/G13 class of G protein signaling pathways and sometimes it works forming a complex with other proteins. It has been implicated in cellular interactions with the cytoskeleton and affects the organization of actin filaments. These actions influence the control of cell shape and motility suggesting a role in cellular migration and adhesion phenomena.
- Pathways
GNA12 participates in the Rho family of GTPases signaling pathway and the MAPK/ERK pathway both important for cellular response and adaptation. Within these pathways GNA12 activates the Rho guanine nucleotide exchange factors which then influences Rho-dependent processes. It also interacts with other proteins such as GNA13 and RHOA linking it to signal transduction mechanisms that modulate various cellular outcomes including growth and differentiation.
- Associated diseases and disorders
GNA12 links to cancer and pulmonary hypertension. Its role in cancer relates to its influence on cell migration and invasion which are processes involved in cancer metastasis. Studies have shown that changes in GNA12's function can contribute to oncogenic transformation. In pulmonary hypertension altered GNA12 signaling may disrupt normal vascular smooth muscle function contributing to disease progression. The protein connects with RhoA and MAPK/ERK pathway components in these contexts indicating its involvement in the pathology of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-GNA12 antibody (ab236617)
All lanes: Western blot - Anti-GNA12 antibody (ab236617) at 1/500 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4: A549 (Human lung carcinoma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GNA12 antibody (ab236617)
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for GNA12 using ab236617 at a dilution of 1/133 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA12 antibody (ab236617)
Paraffin-embedded human liver cancer tissue stained for GNA12 with ab236617 at a 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA12 antibody (ab236617)
Paraffin-embedded human placenta tissue stained for GNA12 with ab236617 at a 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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